performing these tests was to distinguish six different microbes from one another and to compare how their metabolic and biochemical processes differ from species to species to determine the unknown sample. The tests included: Triple sugar iron agar (TSAI)‚ the Sulfide Indole Mobility (SIM) test‚ Glucose fermentation‚ the Methyl Red test‚ the Voges-Proskauer test‚ Citrate test‚ the Urease Test‚ and finally the Gelatin test. The microbes that were tested during this lab were: Escherichia coli
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3. With a pipet‚ inoculate the agar dish labeled water with the water sample. Use only enough water to cover the top surface of the dish (approximately 4 drops). 4. Cover the dish and let it sit for 30 minutes to ensure the water soaks into the agar. 5. Incubate the dish upside down at room temperature for 24–72 hours. 6. Observe the dish and count the number and types of colonies. Record the results in Data Table 1: Environmental Colony Formation‚ in the Lab Report Assistant section. 7. Soak
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Teeter Foaming Hand Soap is more effective in reducing bacterial growth than the Soft Soap hand soap‚ then there will be less bacterial/microorganism growth present on the agar plate for the Harris Teeter Foaming hand soap. II. Materials and Methods c. Obtain 3 agar plates and 2 different brands of hand soap. Label each agar plate and draw a line to separate each plate into 2 sections (control and experimental). d. Touch control side of each plate with hands rinsed only with water
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A. Describe the difference between an obligate and facultative aerobe. An obligate aerobe is a microorganism that must have oxygen for growth and will grow at the top of the test tube. A facultative aerobe prefers oxygen but it is not necessary for growth. Many of these microorganisms will grow at the top of the tube‚ but will also be dispersed throughout as well. B. Discuss the temperature requirements of the organisms (you grew) in this lab. Both Staphylococcus epidermis and Lactobacillus acidophilus
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NAME: PRAJWAL BHARADWAJ Ampicillin‚ Vancomycin‚ and Tetracycline Effectiveness on Gram Positive and Gram Negative Bacteria. INTRODUCTION: Bacteria are microbial organisms which are present in various environments. Many bacteria are good and help humans synthesize materials and assist in biological processes‚ such as digestion. However‚ some bacteria can cause harmful diseases. When harmful bacteria infects the body‚ people take antibiotics in order to suppress bacteria. Different antibiotics have
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is conducted to identify the bacteria as gram negative or gram positive. Two other stains were carried out. To determine the motility of the bacterium‚ wet mounts of the bacterium were observed and the motility was confirmed by using soft agar plates and soft agar deeps for
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As community succession occurs in bacterial colonies pH‚ odor‚ color‚ and consistency changes take place. In this experiment‚ four different milk age samples‚ fresh‚ 24 hour old‚ 4 day old‚ and 8 day old milk‚ will be prepared on different sets of agar plates that will be diluted to different levels for optimum bacterial growth and measurement. To identify the resulting colonies a procedure called gram stain is used in the identification of bacteria. Bacteria can be gram-positive‚ gram-negative
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same temperature and to make sure it is not too hot to kill the bacteria and not too cold to prevent the solution from combining. Amount of bacteria used on each agar dish. This is to make sure that no brand of detergent is either more advantaged or disadvantaged than the other in its ability to kill the bacteria. Amount of time each agar dish spent in incubation and the temperature of the incubator. To ensure the bacteria has an equal chance of growing within the same timeframe. Materials 4 beakers
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ABSTRACT The effect of molecular weight on the rate of diffusion was measured using two tests namely: the glass tube test and the agar-water test. The set-up of the glass tube test used two cotton balls of the same size. One cotton ball is moistened with hydrochloric acid (HCl) and the other one is moistened with ammonium hydroxide (NH4OH). The two cotton balls were inserted in both ends of the glass tube. NH4OH which has a lighter molecular weight (35.0459 g/mole) diffused with a faster rate (dave=20
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A streak culture was performed on a Tryptic Soy Agar Plate for isolation and purity and then incubated at 37 degrees Celsius for 24 hours. A gram stain was performed to determine whether the bacterium was a gram negative or a gram positive. After performing the gram stain‚ I concluded that by the appearance of purple spherical clusters resembling grapes that is was a gram positive cocci. A Catalase test was then performed using hydrogen peroxide. A positive catalase test was observed
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