his experiment was done to find out if garlic is effective in killing bacteria. This will help us understand the effectiveness of home remedies such as the use of natural herbs (including garlic) for medicinal purposes. Hypothesis Garlic will kill bacteria and prevent its further growth. Background Herbs and spices have traditionally been used in cooking as they add flavor to our food. These herbs and spices have also been known to many cultures for their medicinal properties. The oils that are
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For the completion of this experiment the procedures were guided with the Rainbow Transformation1 lab manual. An Escherichia coli bacterial reference plate was used to obtain colonies which were resuspended into a CaCl2 solution that was previously kept on an ice bath. The rainbow transformation mixture containing the plasmid DNA was then added to half of the E. coli cells. These cells were later placed into a water bath set to 42ºC and “heat shocked” to promote the entrance of DNA into the cells
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Difco & BBL Manual Manual of Microbiological Culture Media Second Edition Editors Mary Jo Zimbro‚ B.S.‚ MT (ASCP) David A. Power‚ Ph.D. Sharon M. Miller‚ B.S.‚ MT (ASCP) George E. Wilson‚ MBA‚ B.S.‚ MT (ASCP) Julie A. Johnson‚ B.A. BD Diagnostics – Diagnostic Systems 7 Loveton Circle Sparks‚ MD 21152 Copyright 2009 Becton‚ Dickinson and Company 7 Loveton Circle P.O. Box 999 Sparks‚ Maryland 21152 ISBN 0-9727207-1-5 All rights reserved Printed in the United States of America AOAC
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unknown was inoculated into a TSI slant by stabbing the specimen into the butt using a needle and then streaking on the surface of the agar. The sample was allowed to incubate for 24 hours and read for results. The next test was the citrate utilization slant which examines the ability for a bacteria to use citrate as the sole carbon and energy source. The citrate agar tube was inoculated with the bug by using a needle and then streaking over the surface of the slant. Upon an incubation period of 48
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Antibacterial activity of allopathic medicine versus phytomedicine against Enterococcus faecalis: An in-vitro study. Abstract Background: A successful endodontic treatment depends on appropriate instrumentation‚ irrigation‚ decontamination and obturation. Decontamination of root canal plays a crucial role in the success of endodontic treatment. Therefore‚ a suitable medicament is essential which can effectively eliminate bacterial contamination. Aim and objectives: To investigate and compare the
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Lab Report T 1:35 Prof. Clemente Fernandez MCB2010L 11/19/13 Cristina Carvajal Fatima Hussain Nordia Johnson Jessica Rignack Amoeba: It is a unicellular protozoan that lacks a definite shape. Live in both fresh water and marine habitats. Some are found in soil‚ and many have adapted to parasitic life on the body of marine animals or internal organs of both aquatic and terrestrial animals. Amoeba reproduces asexually by binary and multiple fissions. They eat algae‚ bacteria‚ other
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sociological groups by employing interpretive and naturalistic approaches. It is both observational and narrative in nature and relies less on the experimental elements normally associated with scientific research (reliability‚ validity and generalizability). Agar (1980) suggests that qualitative inquiry relies more on appearance‚ verisimilitude and transferability. On the other hand‚ Bosk (2001) emphasizes the importance of credibility‚ transferability‚ dependability and conformability in qualitative studies
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Obtain agar plates from your instructor and label them as in the figure 1 provided below. 2. Using a sterile pipet‚ remove 130µL mixed bacterial suspension from the tube “C “‚ remove the lid from the control plate and dispense the bacteria unto the agar. Use a cell spreader to spread the bacteria evenly onto the agar surface. Use of the cell spreader; Dip the cell spreader into ethanol. Pass the spreader
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Floyd and Dr. Kennedy (1) unless otherwise noted. The first step that was used was a three streak method‚ described in Lab 4‚ on a Blood Heart Infused/Trypticase Soy Agar plate to isolate the two unknown bacteria. Once the two bacteria were incubated‚ grown‚ and isolated they were each individually streaked on a Trypticase Soy Agar plate to isolate individual colonies to be studied‚ tested and identified. After incubation of the individual TSA plates‚ the morphologies were viewed and noted and
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quantitative Ames test is similar to the spot-overlay Ames test‚ except that the mutant Salmonella tymphimuium (which cannot composite histidine) is treated with various concentrations of the mutagen Diet Coke to view which sections of the Davis Minimal Agar Plate form colonies (Bjeldanes‚ et al. 1982). Mutagenicity has been known to be carcinogenic and that can cause a toxicological effect that presents a potential health risk for humans ( Resende‚ et al. 2012). The purpose for doing this experiment
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