Read in your lab manual about the following agar mediums: Blood Agar (pg 168)‚ EMB Agar (pg 170)‚ Mannitol Salt Agar (MSA)(pg 172) )‚ MacConkey Agar (pg 174)‚ and PEA Agar (pg 176) to answer the following: 1. What does the blood agar select for? Blood agar allows distinction among bacteria based on their ability to lyse red blood cells (hemolytic activity). 2. What color is the blood agar? Blood red color. 3. What are the 3 types of blood agar results and how can you recognize them?
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Agar What is it and where is it from? Derived from the cell walls of red algae‚ agar is a gelatinous substance extracted through a process of boiling and filtration. Agar is specifically used in laboratories for diagnostics and experimental purposes. Agars main purpose is to act as a growth medium for micro-organisms such as bacteria and fungi. The micro-organisms feed off the nutrients contained in Agar and can be both cultured and observed within it by scientists and the use of a microscope. Using
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certain situations. Different samples of bacteria cultures were gathered from different places such as the mouth‚ shaded mulch‚ and from the table top. The samples were collected by using a cotton swab and swiped onto a petri dish filled with nutrient agar. The bacteria were then left to grow on their own in a relatively well shaded area. As a control‚ a cotton swab that had nothing on it was used. It was recognized that different types of bacteria exist in different environments and grow in different
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Materials • 30 petri dishes • Nutrient agar (a culture containing agar used to grow bacteria) • Sterile paper disks • Bacillus subtilis broth • Home-built incubator (a sealed box with a heat pad underneath) • Light box • Penicillin • Streptomycin • Tetracycline • Glutaraldehyde • 5 percent phenol alcohol • Fresh garlic extract Procedures 1. Divide petri dishes into six groups of five. 2. With adult supervision‚ melt the agar medium. 3. When liquid agar cools‚ pour an equal amount into each
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- 3 Petri dishes prepared with agar - 1 disinfected swab - 1 bottle of disinfected water - A piece of filter paper - A hole puncher - 4 test tubes - 1 measuring cylinder - 1 pipette with disposable tips - Tetracycline - Clindamycin - Benzoyl peroxide - 1 beaker of water - P. acne bacteria culture - 1 forcep - 1 digital weighing scale - 1 marker pen 1. Before starting the experiment‚ make sure you clean your work area with Chlorox and wear gloves at all
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Hypothesis: The hypothesis for this particular practical experiment is that‚ as the pH decreases or increases from neutral pH of approximately 7‚ the amount of microbial growth on the agar solution will decrease Materials: Agar Solution Preparation: *hand sanitizer/ hand wash soap *small container *1g of agar powder *0.25g of beef extract stock *60mL of heated water *electronic balance *small spoons (2x) *teaspoons (2x) *0.2M Acetic Acid Solution (pH of 3) *0.2M Sodium Hydroxide Solution
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satisfactory method employed to evaluate the effectiveness of antiseptic agents against selected test organism. In this method‚ sterile filter paper disk is impregnated with an antiseotic and placed on fresh heavily inoculated agar plate of a test organism. Following incubation‚ the agar plate is looked for a zone of inhibition. The presence of a clear zone of inhibition surrounding the disk is indicative of inhibitory (antimicrobial) activity against the organism. This experiment deal with the evaluation
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microorganisms. Gain more knowledge about selective media and differential media. Practice use of the catalase test‚ coagulase and the oxidase test. Observe microbial flora of the nose. Significance: Understand the use of Mannitol salt agar‚ blood agar and MacConkey agar plates which must be used based on the components of the bacteria. The catalase test will be used to understand the difference in facultative anaerobic and groam positive from aero tolerant anaerobes. The coagulase test converts fibrogen
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on a MacConkey agar plate. The first part of the experiment involved the methods of manipulating‚ identifying and counting the bacteria and the second part was to find out whether the bacteria E.coli was the only type found in the given area by gram staining. E.coli was the chosen bacteria for this type of experiment. It is a gram negative bacterium that will grow rapidly given ‘any culture medium with the necessary energy source‚ nutrients‚ pH‚ and temperature’. Therefore‚ MacConkey Agar being
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alcohol solution‚ 1 Zip bag‚ 1 Pan to heat agar‚ 1 Isopropyl alcohol (rubbing alcohol)‚ 1 Cultures: S. epidermidis and L. acidophilus‚ 1 Gloves‚ Disposable‚ 1 Pencil‚ marking‚ 11 Petri dish‚ 60 mm‚ 2 Candles (flame source)‚ 1 Thermometer-in-cardboard-tube‚6 Test Tube(6)‚ 16 x 125 mm in Bubble Bag‚ 1 Test tube holder‚ 1 Test-tube-rack-6x21-mm‚ 1 Pipet Graduated Small (5 mL)‚ 1 Baker’s Yeast Packet – Saccharomyces cerevisiae‚ 1 Agar‚ MRS - 18 mL in Glass Tube‚ 4 Agar‚ Nutrient - 18 mL in Glass Tube‚ 1 Broth
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