of incubation‚ results were collected as growth or no growth for each temperature. Methods and Materials: Figure 1a: agar plate Materials 6 nutrient agar plates media grown overnight in Tryptic Soy Broth E. coli P. fluorescens B. stearothermophi lis Inoculum loop
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ii) Tube of sterile distilled water. iii) Petri dishes with nutrient agar iv) China Marker v) Object to be tested (all are from the front of the class room): (1) Teachers Chair (2) Computer Keyboard (3) Computer Mouse (4) Teachers Desktop b) Method: vi) draw‚ with a china marker‚ on the outside of the agar portion‚ not the lid‚ of a Petri
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with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin). The extracted plasmid DNA is important as it contains ampicillin-resistant gene. As such‚ E. coli cells that have taken up this plasmid DNA will be resistant to ampicillin and survive‚ hence growth of colonies will be observed on the agar plates. One of the rationales behind heat shock method is to create pores‚ allowing uptake of plasmid DNA (Panja et
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practical. Materials used in the practical consist of blocks of agar jelly containing indicator‚ a sharp knife‚ used to cut the agar jelly into the recommended sizes‚ diluted sulphuric acid (300mL) ‚ ruler‚ to measure the sizes of the cubes‚ absorbant paper towel‚ three 250mL beakers‚ and a measuring cylinder. To perform this experiment‚ the following procedures will need to be follow precisely: Obtain three blocks of agar jelly. Carefully cut three cubes‚ as close as possible to 1 cm‚ 2
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Review all chapters in your exercise manual beginning with the introduction INTRODUCTION: (Covered 9.4.14 II Week 1) Biosafety levels1: basic level of containment. Hand washing or wearing gloves 2: Appropriate for working with human body fluids. Autoclave‚ sharps containers‚ lab coats 3: appropriate for working with pathogens that can be transmitted via respiratory route. Self-closing‚ double doors and sealed windows 4: Highest level. Aerosol pathogens; pathogens with no vaccine/treatment. Separate
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Using Selective Media to Monitor the Fermentation Process of Cabbage and Cucumber‚ for Fourteen Days Abstract: Introduction: The goal of the ecological succession lab is to demonstrate succession with the fermentation of cabbage and cucumbers. The cabbage will ferment into sauerkraut and the cucumber will ferment to pickles during fermentation process that will changes the species structure and the community of time. During the fermentation process we watched the pH become more acidic‚ since
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Materials: A. Formulation of Hypothesis a. Apparatus - glass tube - cotton balls b. Substances - hydrochloric acid (HCl) - ammonium hydroxide (NH4OH) B. Testing the Hypothesis Concerning the Rate of Diffusion a. Apparatus - petri dish of agar-water gel with three wells b. Substances - potassium permanganate (KMnO4) - potassium dichromate (K2CR2O7) - methylene blue III. Procedure: A. Formulation of Hypothesis 1. Fasten the glass tube to a ring stand. 2. Simultaneously
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aureus nasal carriage rates were found 28% (41/144) in normal population‚ and 31.5% (12/38) in hospital laboratory personnel. Materials: * Mannitol salt agar (MSA) plate – Mannitol is a carbohydrate that can be used by some bacteria as a nutrient. The use of mannitol is important in identifying Stapphylococcus species. Mannitol salt agar contains 7.5% sodium chloride (salt) whereas‚ most media contains about 0.5% sodium chloride. Organisms that cannot tolerate a high salt concentration will not
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Universita Jambi merupakan salah satu universitas negri yang ada diprovinsi jambi‚ yang berdiri pada tahun 1963 tepatnya dibulan Maret tanggal 23. Gedung pusat UNJA berlokasi di desa mendalo‚ kecamatan Jaluko‚ Kabupaten Muaro Jambi‚ Provinsi Jambi. UNJA pusat merupakan universitas yang tidak hanya memiliki gedung-gedung yang digunakan untuk aktivitas perkuliahan‚ tetapi juga merupakan satu-satunya universitas yang berada di Jambi yang memiliki hutan yang masih cukup terjaga kondisinya‚ karena sebagian
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Today‚ certain antibiotics are used in fighting certain diseases caused by pathogenic bacteria such as Salmonella enterica and Escherichia coli. However‚ due to the improper usage of these antibiotics and the increasing antibiotic resistance of the bacteria‚ a lot of the medicines commonly used before to fight the same bacteria are now considered ineffective and because of it‚ scientists try to find ways such as discovering new antibiotics or recommending to the citizens some procedures and ways
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