Biodegradation of Deltamethrin from S1 Fungal Isolate Janice Dsouza‚ Surajvanshikumar Suvarna‚ Seekha Parida‚ Anudurga Gajendiran and Jayanthi Abraham VIT UNIVERSITY VELLORE Abstract: Microbial biodegradation is a promising approach for recovery of environmental sites contaminated with pesticides and an effective way to remove toxicants. Deltamethrin is the most commonly used pyrethroid in agricultural practices commonly used in different geographical parts of the world. It is detected in many environmental
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Sukses Berwirausaha: Bangkit dari Kegagalan Ada pepatah kuno yang mengajarkan pada kita bahwa sesuatu yang kecil‚ kalau kita tekun membesarkannya‚ lama-lama akan menjadi besar juga. Sedikit-sedikit lama-lama menjadi bukit. Semakin besar bisnis yang ingin kita capai‚ maka kita perlu menyederhanakannya dengan mulai melangkah‚ sekecil apapun langkah itu. Banyak pengusaha yang memulai dari bisnis kecil-kecilan namun perlahan-lahan kemudian menjadi pengusaha besar. Lalu kita hanya membutuhkan kesabaran
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The agar contains Triphenyl tetrazoliumchloric (TTC) which gives off a color indicator for motility. If the organism moves from the stab indention‚ then the inoculation shows it was motile and grew red in the agar. However‚ if the organism did not move from the stab indention‚ then it will appear just a thin precipitate of red‚ which indicates a non-motile growth
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C₁₆H₁₈N₃SCl) of different molecular weight to agar-water gel‚ and measuring the distance of diffusion every three minutes for 30 minutes. Ideally the lighter substance would spread at a faster rate. However‚ C₁₆H₁₈N₃SC‚ the one with highest molecular weight‚ had a highest rate of diffusion. It is either (1) the hypothesis is wrong and thus rejected‚ or (2) parts of the experiment ’s conduct was incorrectly done‚ such as the amount of the substance droped into the agar-water gel. INTRODUCTION Diffusion
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Usually the media is based on a known environmental condition range that they tolerate and most groups can not. An example is Manitol Salts Agar (MSA). This media contains salts that most organisms can’t tolerate due to their osmolity ranges. Microbes that normally exist under these conditions are able to grow. MSA is usually used for Staphylococcal species. Differential media provides chemical
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Materials and Methods: A stock culture‚ labelled 25‚ was stored at room temperature (in the lab desk). A transfer loop was sterilized and a sample of the stock was transmitted to a tube of sterile BHI broth (for a working culture) and also to an agar plate and incubated for 24 hours. From the incubated plate‚ distinct and well isolated waxy‚ yellowish colonies appeared. Another sample was applied to a glass slide‚ heat fixed and a Gram stain test was performed. From the list of several possible
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and Methods The procedure for all tests performed was taken from Leboffe and Pierce’s Microbiology: Laboratory Theory and Application. The first and easiest data to record is the color of the bacterial colonies on a nutrient agar plate. Growing the cultures on the agar can be done by streaking a plate so as to isolate the bacterial colonies. Then separate the two colonies that are different in growth on a TP plate by using the same streaking techniques with a sterile loop. Both bacteria will then
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its characteristics. This same approach can be used when testing for resistance evolution within bacterial strains. Our hypothesis states that bacteriophage will not be present within our sample from East Lake. With the use of a phage buffer and soft agar‚ it provides an even suspension and growth of cells. This is essential for seeing lysis caused by bacteriophage. After 24 hours‚ our data seemed significant as signs of successful infection within the bacteria was not present. No plaques were formed
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The purpose of this lab was to observe the rate of osmosis and diffusion‚ as well as the effect of molecular size of the particles on this rate. Part I of the lab was a demonstration of osmosis and diffusion‚ that dealt with raisins in different liquid environments‚ each with a different concentration of sugar. Part IV of the lab was using the same idea as the demonstration‚ by putting objects in different concentrations of a substance; in this case elodea leaves in salt water. In both cases‚ the
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of. The materials we used to make this test possible consists of a sterile nutrient agar Petri dish‚ the bacteria Serratia marcescens‚ Erythromycin antibiotic disk‚ Ampicillin antibiotic disk‚ Penicillin antibiotic disk‚ sterile disk of blank filter paper‚ marking pen‚ long handled cotton swab‚ forceps‚ metric ruler‚ and a 37°C incubator. For our procedure we first filled our sterile Petri dish with agar‚ just so that the bottom of it was covered‚ then waited a day for it to condense. Then
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