facilitates the handling of large numbers of bacterial clones for classification on a variety of media. METHODS Replica plating. A frequent chore in bacteriological work is the transfer of isolates from one substrate to other selective or indicator agar media. In place of an inoculating needle‚ one might imagine a device consisting of many needle tips in fixed array‚ so that one operation would substitute for repeated transfers with a single needle. The requirements of this design are met by pile
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and starch agar. OBJECTIVES: distinguish between different bacterial species based on colony morphology on agar plates To distinguish the growth characteristics of microorganisms in various differential‚ and selective media. Differentiate bacteria based on their ability to hydrolyze starch. Materials: Plates of EMB‚ Starch and blood agar. Stool sample. Inoculating loop. Bunsen burner. Soil sample. Cotton soap. Skin sample. Gram iodine. Results: Starch agar: Special
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Materials: 4 sterile cotton swab‚ sterile water in test tube‚ 4 agar plates and 2 blood agar plates‚ wax pencil and labels. Procedure: Appropriately label the cover of each plate as indicated in lab. 1. Determine 2 sampling sources‚ one from your body and one from the surrounding environment. 2. For the first agar plate‚ for sampling from air‚ remove the lids from the plate and allow it to sit uncovered for 15 minutes. 1. For second agar plate‚ open the “stick” end of the sterile cotton swabs to
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observable microbial colonies on the surface of the Agar solid‚ as to determine the presence of microbes in consumable products i.e. yoghurt and blue vein cheese. HYPOTHESIS: Microbial growth will be present in two of the three Agar plates (those containing the food product) due to the suspected presence of microbes‚ whilst the control Agar plate (containing no food products) will remain free of contamination and microbial growth. MATERIALS: - 3x Agar plates - 10g of berry yoghurt - 10g
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An agar plate is a Petri dish that contains a combination of agar and nutrients that help microorganisms grow. The proper method of setting microorganisms on an agar plate is know as “streaking”. In order to streak‚ the microorganisms are placed on a sterile swab or metal wire‚ which is then dragged lightly against the agar solution‚ leaving behind the microorganisms. The amount of organisms is greatest at the beginning
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Purpose The principle of this experiment is to have a thorough perceptive of: • Culture washed and unwashed lettuce on agar plate. • Culture fresh and opened milk with the same expiration dates. • Explain the significance of food safety. • Illustrate foodborne sicknesses.
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that will be of use to you throughout your biological work. Procedure 1. Heat the test tubes of sterile agar medium in the water bath until the agar melts. 2. Remove the test tubes from the water bath. Let them cool enough to hold in your hand‚ but not so much that the agar becomes solid again. Perform the following transfer as quickly as possible. You must work rapidly so that the liquid agar will not cool and solidify before the transfer is completed. 3. Hold both a test tube
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facilities and food products which to be analyzed are swabbed. The swab are diluted in a dilutant such as peptone water or phosphate buffer‚ according to the anticipated amount of contamination and subsequently applied to a growth medium containing agar in a sterile‚ covered plate (David‚ Richard and R. 2004). There are many advantages to the cotton swab method. These include the ease with which any health care
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2 agar plates divided into 4 equal sections were used for this experiment. Each section was labeled with a number from 1-8. 8 Sterile swabs were used‚ 1 for each surface swab. 8 surfaces in my home were then identified that could serve as a fomite and swabbed with a sterile swab that was dipped in distilled water to moisten it. Surface #1 was the garbage disposal in the kitchen sink. It was swabbed and the microbes transferred to the appropriately labeled section marked #1 of the agar plate
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In this lab you will test the idea that microbes are ubiquitous or present everywhere at all times. Materials One Triptic Soy Agar (TSA) plate per student One sterile swab Procedure 1. Decide on an area to test for the ubiquity of microbes. List your area on the white board in front of the classroom. 2. Obtain one TSA plate and label it on the bottom (side with the agar) with your name‚ class section and the surface you will sample. 3. Obtain one sterile swab. 4. To obtain a sample‚ roll the sterile
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