utilizing procedures learnt during the semester. Procedures were followed as stated in the lab manual (1). Since the sample contained two unidentified bacteria‚ the first step was to isolate each bacterium using streak plate technique. Tryptic Soy Agar (TSA) plate‚ and differential media such as mannitol salt and Eosin methylene blue (EMB) were used for isolation streak technique. This step is imperative because the bacteria need to be separated and isolated before they can be identified. Moreover
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CHAPTER I INTRODUCTION Background of the Study Mangrove swamps are forested intertidal ecosystems that occupy sediment-rich sheltered tropical coastal environments. By trapping and stabilizing fine sediments‚ mangroves control the quality of marine coastal waters. Aside from maintaining coastal food webs and populations of animals‚ mangroves have an important role in pollution control through their absorptive capacity for organic pollutants and nutrients‚ and they play an important role in storm
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SEROLOGICAL‚ CULTURAL AND MOLECULAR DETECTION OF BRUCELLA INFECTION IN BREEDING BULLS A THESIS SUBMITTED TO THE ANAND AGRICULTURAL UNIVERSITY IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF THE DEGREE OF Doctor of Philosophy IN VETERINARY MICROBIOLOGY BY AMIT N. KANANI M. V. Sc. DEPARTMENT OF VETERINARY MICROBIOLOGY COLLEGE OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY ANAND AGRICULTURAL UNIVERSITY ANAND-388001 (GUJARAT) 2007 Reg. No. 04-05194-2001 Dr. J. H. Purohit
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Work Plan for Isolation‚ Purification‚ Identification and Starter Culture Activity of Lactoccocus lactis Submitted by: M.Usman Akram B.S. (Hons.) Dairy Technology mh.usman@hotmail.com Mobile : +923217773736 University of Veterinary and Animal Sciences‚ Ravi Campus Pattoki Lactoccocus lactis Classification: Scientific classification | Kingdom: | Bacteria | Division: | Firmicutes | Class: | Bacilli | Order: | Lactobacillales | Family: | Streptococcaceae | Genus:
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Objectives 1. To determine biochemical activities of microorganisms. 2. To observe the product of biochemical activities of microorganisms. 3. To learn the skills of inoculation agar tubes and agar plates. Introduction Microorganisms are able to carry out different biochemical activities with the ease of different enzymes. Each of these enzymes carries out one specific type of the chemical transformation. They convert substrates into product. A) Carbohydrates Fermentation Microorganisms utilize
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Research Plan Title: In vitro Antibacterial Activity of Santol (Sandoricum koet jape) at Different pH of Agar School : Ramon Magsaysay (Cubao) High School School Address: Ermin Garcia St.‚ Cubao Quezon City Research Adviser: Mr. Ryan D. Balandra Statement of the Problem The aim of the study is to identify the effect of different pH level of the Agar plate to the antibacterial activity of Santol (Sandoricum koet jape). Specifically‚ the study will seek for the answer of the question:
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Unknown Identification Report The objective of this experiment was to identify an organism from a mixture of two unknown bacterial species. In order to accomplish this‚ I first plated my unknown mixture on Tryptic Soy Agar (TSA)‚ Columbia Naladixic Acid (CNA)‚ and MacConkey’s Agar (MAC) plates. After 48 hours of incubation‚ it was unclear that two different bacterial colonies had grown on my TSA plate. Only one type of colony was evident. However‚ it was apparent that I had successfully isolated
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Course: BIO-205 BD2 Microbiology Instructor: Dirk VandePol Date: 6/21/2013 Streak Plate Isolation for Obtaining Pure Culture 1. When an agar plate is inoculated‚ why is the loop sterilized after the initial inoculation in put on? Ans: We use agar plate to inoculate microbes by zipping the loop on the agar several times. We streak on the agar plate four time‚ propose is to isolate the unknown bacteria. Therefore‚ the first time to streak on the plate‚ there are million of bacteria on the
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IDENTIFYING GRAM POSITIVE COCCI As mentioned in Exercise 8‚ “Identifying Gram Negative Rods”‚ identifying bacteria is a common activity in the microbiology lab. Like the game Clue™‚ each time you gather a piece of information to solve the mystery‚ you gather some information that supports some identities and eliminates others from contention. In the lab‚ the process continues as you gather more information until only one microbe remains and all others have been eliminated as possibilities. Thus
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Clare Worley 6S 4 - 16 - 18 What Surface in OLV has the most Germs? Introduction I have tested several surfaces in Our Lady of Victory to see which surface has the most germs. I tested ten surfaces and let the petri dishes grow for five days. Over the five days I counted the spores each day and took pictures every other day. It is amazing how things can look and feel clean but they are actually filthy. It is also crazy how many surfaces we touch every day and how one
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