An Le Foundation Biology lab BRADFORD PROTEIN DISCUSSION ESSAY The appearance of blue color showed the present of protein in the BSA dilutions. The more diluted the solution was‚ the less blue it was. The R2 value of the standard curve of BSA dilution was obtained to be 0.9972‚ which is close to 1. The closer to 1 the R2 value was‚ the more accurate the linear portion was. The error percentage of each unknown was large: 25.9% for skim milk‚ 95% for soy sauce‚ and 64.7% for egg white. The vast
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Puritan view William Bradford was the leader of one of the earliest colonial settlements in the United States‚ of which Thomas Morton was a settler. Bradford documented his exploits in “Of Plymouth Plantation” to gain support from his home country and fellow colonists. Bradford’s work‚ however‚ differs from Morton’s “New English Canaan” which also describes the events which took place in the colonies and his views of the Cavaliers‚ the Indians‚ and the Pilgrims. Bradford applies his rhetoric
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after verse being quoted from the Bible. Some people have many Gods while most have one reining all-powerful being. In the case of Mary Rowlandson‚ I believe her being a puritan and having the strong puritan beliefs made her more suitable for dealing with the trails she was about to face then say a everyday church goer. Being the wife of a preacher it shows in the text Mary was solidly grounded in her faith making and well knowledgeable The Puritan Belief is one that was quite popular in the colonies
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This essay summarizes the key aspects of Rowlandson’s captivity story; the reasons behind her captivity; how she juxtaposes the bible and her experiences; the trials and tribulations that she had to confront in the hands of her captors; the type of succor that she received during her moments of crisis; her attitude towards her Native Americans captors; the culture‚ traditions and attitude of the her captors namely the Algokian Indians; the hardships the Indians had to endure at the hands the colonists;
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said “The American is a new man‚ who acts upon new principles”. (Crevecoeur‚ 2007‚ 599) These convictions are brought about by the environment and the courage to leave the old world behind. One might say Crevecoeur defines the new American‚ while Bradford gives us detailed examples into the lives of the early Americans. The European settlers were molded by their new environment‚ as well as the journey they endured to this new land. The seed or the stepping stone for these settlers transformation
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reduced to Cu1+ by forming a complex with protein amide bonds. In the second step‚ Bicinchoninic acid (BCA) forms a purple complex with Cu1+ which is detectable at 562nm. this assay is relatively slow unless heated. When comparing these methods to the Bradford assay‚ the Lowry method has many interfering substances‚ it is a slow reaction and the proteins can irreversibly denature. The BCA assay‚ is also a slow reaction and requires more time ranging from 40minutes to 24hours. Question 8: UV absorption
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The Captive Differences between Rowlandson and Smith There was a time where Native Americans ruled the plains with an iron fist‚ a time where their authority was unmatched by civilized law and when puritans and early settlers alike shook with fear and respect for their Barbaric Neighbors. Why did the Natives show such ferocity? Perhaps the early settlers‚ not only forced their beliefs‚ but forced the natives out of their land as well. Anger‚ anarchy‚ and revelry spread like a plague and in the midst
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Eve‚ Mary-mother of Jesus‚ and Mary Magdalene are all prominent characters in the Hebrew and Christian Bibles and to some extent are mentioned in the Quran. Regardless of whether or not a person believes these women really existed as portrayed within these religious texts‚ they had and still have a major impact on societal views towards women today. For women to be truly liberated and treated as equals to men requires the circumvention of conventional patriarchal‚ anti-feminist interpretations and
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The Cohen-Bradford Influence model offers a practical approach of reciprocity and exchange; trading what you have that the other person desires in exchange for what you need to accomplish workplace and personal goals. Personally‚ most of my authority comes from this
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Compare and Contrast The Romantics: William Blake and Mary Wolstonecraft Mary Wollstonecraft’s A Vindication of the Rights of Woman sets out to invalidate the social and religious standards of her time in regards to gender‚ just as William Blake sets out to do the same for children. Both Blake and Wollstonecraft can be read by the average man and woman‚ lending its attention toward both upper and middle class. Wollstonecraft’s revolutionary themes of tyranny and oppression of women parallel
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