Protein Concentration using BCA and Bradford Assays Introduction There are several ways to determine protein concentration for a sample such as The Lowry Method‚ Bradford assay‚ and Bicinchoninic Acid (BCA) just to name a few. Determining the protein concentration in your sample is an important step in any laboratory workflow that involves protein extraction and/or analysis (1). Based on the assay being used‚ different chemicals can alter the accuracy for the results. For example‚ in BCA‚ the reducing
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When Thomas Bradford woke up that morning‚ he realizes that the bed was too small for him because part of his legs were outside of it‚ but his hands were as small as always. He looked through the big window of his big room and he realizes that it was still dark outside and very cold‚ he was surprised because it was summer time and every day before this one had been sunny and hot. How could this be? He asked to himself. He had spent his whole life working since he was a kid just to build an empire
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During this experiment‚ the method of Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) was conducted using a negatively charged protein and pre-stained molecular weight markers. The hypothesis was that the molecular weight of N-acetyl-β-D-hexosaminidase B would be 28‚000 kDa. To confirm or reject the hypothesis‚ the molecular weight of N-acetyl-β-D-hexosaminidase B and the concentration of protein had to be determined. The electrophoresis of the protein gel were conducted using
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Reality Check During reality check at Bradford I learn a lot about handling money and the meaning of it. I had a fake life where I made a steady income of $7‚000.00 a month and had to spend that through out a variety of things including housing‚ a car‚ clothes‚ food‚ gas‚ and child support. Also I wanted to plan a budget where I could have extra money for extra things including money to go to the movies or buy a television in my home. I did not have any kids living with me at home so I did
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How Mary Smith and John Smith survived captivity In The Account of Mary Rowlandson Captivity Narrative‚ Mary Rowlandson describes in detail the tragic events she had to face after being taken captive by the Wampanoag’s in 1676. She is certain that the only reason she has been taken captive is because god is punishing her for her wrong doings. Like Mary Rowlandson Col. John Smith also was taken captive against his will. In Col. James Smith Captivity Narrative he is not treated poorly or beaten
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The Feud Between Hamlock and Bradford “In ancient times a kingdom known as Hamford‚ used to be ruled by two powerful families‚ the Lock’s and the Brad’s. These two families operated as a single unit working together to keep their kingdom in order and conquer other lands. But‚ slowly as time passed the Brad’s became consumed by the immense power they held over the lands and soon desired that the power of the kingdom should be controlled by themselves. So the Brad’s formed a group of rouges known
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Mary Shelley was born on August 30‚ 1797‚ becoming a distinguished‚ though often neglected‚ literary figure during the Romanticism Era. Mary was the only child of Mary Wollstonecraft‚ a famous feminist‚ but after her birth‚ Wollstonecraft passed away (Harris). Similar to Mary’s book Frankenstein‚ both her and Victor’s mothers die when they are at a very young age. Mary’s father was William Godwin‚ an English philosopher who also wrote novels that would inspire Mary in the late years of her life (Holmes)
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very prominent woman in America back then. She was hung for defying a Puritan law which banned Quakers from the colonies. This woman’s name was Mary Dyer. Not much is known of Mary Barrett Dyer’s early life. On October 27th‚ 1663 the first known history of Mary Dyer was noted. She married William Dyer‚ a very successful merchant‚ in London. William and Mary added on to the family by giving birth to six children. In 1635 they all migrated to the Massachusetts Bay colony where they had been accepted
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Mary the mother of Jesus is an important figure to the Catholic Churches teachings. Mary is known through many biblical references‚ which are‚ however‚ lightly mentioned to create a proper biography. The development of Mary can be traced through the history of the Christians- apparitions‚ feast days‚ organisations and prayers. The Church doesn’t require belief in any apparition or other private revelation. However‚ she does exercise her maternal judgment for the protection of the faithful in declaring
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The Life and Literary Works of Mary Shelley Mary Wollstonecraft Shelley (August 30th 1797- February 1st 1851) was born in London England and was an editor‚ dramatist‚ essayist‚ and novelist best known for her novel Frankenstein (1818). Her father‚ William Godwin‚ was a political philosopher‚ and her mother‚ Mary WallStonecraft‚ was a philosopher and feminist. Mary Shelley’s mother died when she was 11 days old due to complications from child birth. Although Mary received little formal education
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