Purpose: Restriction enzymes cut DNA at a certain palindromic sequence. Three samples of lamda DNA set up to be cut with restriction enzymes PstI‚ EcoRI‚ or HindDIII. There were also two more samples‚ one of these samples was not mixed with any restriction enzyme and the other was a marker‚ which used an enzyme which creates fragments with a known number of base pairs used to create a standard curve. All five samples were put through agarose gel electrophoresis in order to estimate the amount of
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Title. Restriction Enzyme Mapping of pBR322 Using Agarose Gel Electrophoresis. II. Authors. Author: Partner: Section: Thursday‚ 1:10 pm Date of Experiment: October 25‚ 2012 III. Introduction. Restriction enzymes (or restriction endonucleases)‚ originally isolated from Haemophilus influenzae in 1970‚ are enzymes within a cell that cleave foreign DNA within a specific and predictable nucleotide sequence (known as a restriction site) regardless of the source of such DNA. Such restriction sites
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Essential Restrictions on the First Amendment Limitations on freedoms of society are crucial‚ especially in America. As Roger Rosenblatt noted in his essay‚ “We Are Free to Be You‚ Me‚ Stupid and Dead‚” many people express their freedom of speech in very offensive and controversial ways. Often their expressions violate other amendments and freedoms as well. A few examples given by Rosenblatt included acts of freedom of speech performed by professional sports players. Many believe these were
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CONTENTS Executive Summary ...................................................................................................................................... 1 EXHIBIT 1 – SCENARIO 1: REHABILITATION OF CYNTHIA SELLING EXISTING PARTS & CONVERTING TO STOKER FIRING IN 1950 ......................................................................................... 3 EXHIBIT 2 – SCENARIO 2: REHABILITATION OF CYNTHIA USING EXISTING PARTS & CONVERTING TO STOKER FIRING IN 1950 .......................
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Amber Champion December 1‚ 2012 SPC 2608 In today’s society there seems to be many different restrictions in place. Many people would say too many. For example‚ restrictions on the minimum age requirement before you are allowed to drive a vehicle‚ minimum height requirements for children and adults in order to be able to ride a roller-coaster‚ and how old you have to be in order to legally be allowed to consume alcoholic beverages. Although‚ many of these restrictions are in place to ensure
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There are many social restrictions that cause a person to behave or think a certain way‚ These restrictions lead a person to act a certain way‚ based on the influences present. There are many limitations from many different influences that result in these behaviors. In a community‚ a person is expected to be placed under the chains of social restrictions in order to fit the acceptable standards. In the voting process over time‚ the restrictions/expectations have changed. At first‚ in order to vote
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This experiment is to determine the unknown DNA plasmid using restriction enzymes and conducting electrophoresis finally comparing the resulting fragments with the known restriction map. In this lab‚ it succeeds in showing the fragments. In this report we will discuss the‚ results‚ limitations and possible errors. Introduction In biology restriction enzymes are used in several ways to modify and manipulate DNA molecules. One common use is to compare pieces of DNA from one that is unknown‚ with fragments
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The First Amendment is the cornerstone of American democracy and the primary protector of the freedoms Americans enjoy. While some might argue that there should be no restrictions on the First Amendment‚ the First Amendment should be restricted in order to promote public safety and ensure the smooth functioning of society‚ and those limitations should be narrowly crafted and explicitly defined by the Supreme Court. A person has the right to voice his or her opinion on a matter‚ regardless of what
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BIO 219 Group 1 Section 66 October 19‚ 2012 The extraction of purified DNA from A. fischeri by restriction digestion using Sal I enzyme and pGEM for shotgun cloning Introduction: The ultimate goal of this experiment is to isolate the lux operon‚ a targeted piece of DNA that causes bioluminescence‚ from Aliivibrio fischeri and insert it into the DNA of Escherichia coli in order to make it glow. A. fischeri is a gram-negative bacteria which participates in a symbiotic relationship with
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However‚ the First World War forced them to scrap this policy and increase the restrictions placed on civilians in order to successfully fight the war. They did this in a number of ways‚ and this answer will include: Conscription‚ Censorship‚ restrictions on women and food rationing. It will be argued that the most extensive increase in state control was food rationing. The most extensive increase in restrictions on civilians was rationing of food. Food had to be rationed due to the fact that
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