the hospital recently and brought a sample their infectious disease on an agar plate. A series of tests were completed in order to identify this unknown sample. First‚ the plate was analyzed for its hemolysis characteristics. Afterwards‚ a Gram stain was performed from a sample of the agar plate and the slide was viewed under the microscope. Once‚ the microscopic visual was captured‚ a catalase test followed. Next‚ for further data‚ MSA results were recorded‚ along with antibiotic observations of
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Introduction: The purpose of this lab is to use staining techniques and biochemical testing to identify an unknown bacteria using Bergey’s manual. Bergey’s manual of Systematic Bacteriology is a dichotomous key primarily used to identify a bacterial species. Biochemical tests are used to differentiate different species of bacteria. These tests are effective in determining the characteristics of the microbe being tested. Such characteristics include citrate utilization‚ gelatin hydrolysis‚ nitrate
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Citrate Test: Some bacteria can use citrate as a source of carbon. To test if the unknown bacteria uses citrate as a source of carbon‚ Simmon’s citrate agar was used as the medium on which the bacteria was grown. The Simmon’s citrate agar consists of sodium citrate as the source of carbon‚ ammonium dihydrogen phosphate as the source of nitrogen along with pH indicator such as bromothymol blue. Procedure: The Citratase activity was detected by inoculating the unknown bacteria on the slant surface
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Testable Question: What will happen to different halide salts found in the body if various compounds are added to the halide? How will the halides react? Introduction: For this lab we tested 6 halide salts found in the body‚ and their reactivity when a compound is added. We tested the halides NaF‚ NaCl‚ KBr‚ KI‚ and two unknown halides. To find the reaction of the halide‚ we are adding drops of Ca(NO3)2‚ AgNO3‚ AgNO3 + Na2S2O3‚ and NaOCI + Starch to the halides. Each halide will be separated from
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Introduction For the last week‚ specific tests had to be applied to determine the unknown solid that was given out randomly. Physical tests‚ solubility test‚ characteristic tests‚ NMR spectrums‚ and IR spectrums were all utilized to distinguish the identity of the solid. These assessments and a couple additional tests will be utilized again to determine the unknown liquid that was given to us in class. The other characteristics that will be looked at will be the boiling point‚ refracted index‚ and
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Verifying Differences Between Athletes’ Heart Rates And Non-Athletes’ Heart Rates By Using Step Test Introduction: Different people have different heart rates because of many reasons such as health condition‚ smoking‚ working out‚ among others. This step test is used to compare and figure out the differences between athletes’ heart rates and non-athletes’ heart rates before and after the test. Kuno Hottenrott stated that the normal average heart rate of people ranges from 40 to 100 beats per
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Purpose: To use a set of standardized procedures to test for simple sugars and starch‚ proteins‚ and fats. Hypotheses: A.) B.) C.) Equipment: -Safety goggles -Lab apron -400 mL beaker -Utility stand with ring clamp -Hot plate -Thermometer -10 mL graduated cylinder -Test-tube racks -8 to 16 test tubes -Test tube holder -Medicine dropper -Rubber stoppers -Test-tube brush -Wax pencil -Distilled water
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My partner and I were given unknown number 3 in the laboratory. After performing various tests over the course of a few weeks on our unknown‚ we came to the conclusion that our unknown organism is Klebsiella pneumoniae (K. pneumoniae). K. pneumoniae is a gram negative bacillus shaped microorganism. We observed that K. pneumoniae is a nonmotile organism. We performed multiple tests on our unknown culture‚ therefore we are very confident that it is correctly identified. We identified that K. pneumoniae
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During the lab experiment six different test were conducted. Each test consists of chemicals to be tested using 3 to 10 drops of the additive in order to observe a change. With any experiment you compose a hypothesis; where you make an educated guess. The hypothesis will primarily be‚ which base chemical will produce the greatest change based on the additive. Experiment 6.1 (Starch) when adding the three drops of Iodine to each tube observe the color change. When performing this test the chemical
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Repeat step 4 for each of the remaining salts. 3. Repeat step 4 for each of the unknown metals and predict the identity of the unknown metals. Repeat any of the known metals to help with this prediction. **Wash hands thoroughly before leaving the lab. ** Some of the salts used are poisonous or irritating to the skin. Keep your hands away from your mouth until after you have washed them and have let the
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