Biology Computational Systems Biology Lecture 3: Enzyme kinetics Tue 17 Jan 2006 with the collaboration of Luna De Ferrari 1 Images from: D. L. Nelson‚ Lehninger Principles of Biochemistry‚ IV Edition‚ W. H. Freeman ed. A. Cornish-Bowden Fundamentals of Enzyme Kinetics‚ Portland Press‚ 2004 A. Cornish-Bowden Enzyme Kinetics‚ IRL Press‚ 1988 Computational Systems Biology Summary: • • • • • • 2 Simple enzyme kinetics Steady-state rate equations Reactions of two substrates
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Biochemistry Unit The Kinetics of Alkali Phosphatase Inhibition 1. OVERVIEW This practical builds on the enzymology lab skills you learned in the Acid Phosphatase practical. Again‚ you will measure the initial reaction velocity (V 0) of an enzyme reaction‚ but this time in the absence and then presence of an inhibitor. Last time you used Acid Phosphatase (Prac 1)‚ but this time you will use the enzyme Alkali Phosphatase. These enzymes have different primary (and hence tertiary) structures
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Kinetics of an SN1 reaction: the effect of solvent on rate Object The purpose of this experiment is to determine the rate of hydrolysis in acetone/water (50/50 v/v and 60/40 v/v). Background and Theory An SN1 reaction of tert-butyl chloride takes place in two steps. First‚ the Alkyl Halide will leave the molecule. In this step the bond is breaking‚ which takes a longer amount of time‚ so it will determine the rate of the reaction. As a result‚ it forms a tertiary carbocation‚ since this
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07-Bickman-45636:07-Bickman-45636 7/28/2008 6:13 PM Page 214 CHAPTER 7 Designing a Qualitative Study Joseph A. Maxwell T raditionally‚ works on research design (most of which focus on quantitative research) have understood “design” in one of two ways. Some take designs to be fixed‚ standard arrangements of research conditions and methods that have their own coherence and logic‚ as possible answers to the question‚ “What research design are you using?” (e.g.‚ Campbell & Stanley
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The two different types of energy are kinetic and potential energy. Kinetic energy is the energy a moving object has because of its motion. The kinetic energy of a moving object depends on the object’s mass and its speed. The kinetic energy of a moving object can be calculated from this equation: Kinetic energy (in joules) = ½ mass (in kg) x [speed (in m/s)]² KE= ½ mv² In this equation ^‚ the symbol v represents speed. Example Find the kinetic energy of the ball having mass 0‚5 kg and
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Lecture No. 1 Chemical Kinetics 1.1 The Rate of a Reaction Chemical Kinetics is the area of Chemistry that is concerned with the speed‚ rate or mechanism at which a chemical reaction occurs. Reaction Rate is the change in the concentration of a reactant or product with time (i.e. M/s). It measures how fast a reactant is consumed and how fast a product is formed. 1.2 WRITING RATE EXPRESSIONS Consider the following hypothetical reaction. A + 2B ( 3C + D Rate = - rate
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Leadership Self-Assessment and Reflection Developing the Leader Within You by John C. Maxwell is a practical application to servant leadership. In this book Maxwell outlines the skills necessary to become a great leader‚ or to simply excel from one level of leadership to another. Maxwell suggests ten principles on which the foundation of great leadership is built. In assessing my own leadership skills‚ the principles with which I am most effective are Influence‚ Integrity and People. In order
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Part B Now‚ suppose that Zak’s younger cousin‚ Greta‚ sees him sliding and takes off her shoes so that she can slide as well (assume her socks have the same coefficient of kinetic friction as Zak’s). Instead of getting a running start‚ she asks Zak to give her a push. So‚ Zak pushes her with a force of 125 \rm N over a distance of 1.00 \rm m. If her mass is 20.0 \rm kg‚ what distance d_2 does she slide after Zak’s push ends? Remember that the frictional force acts on Greta during Zak’s push and
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OBJECTIVE The objectives of this experiment are to investigate and verify the Thevenin’s theorem and to investigate and verify the Norton’s theorem. EQUIPMENT Resistor 100Ω‚ 1kΩ and 4.7kΩ‚ digital multimeter(DVM)‚ bread board. INTRODUCTION Some circuits require more than one voltage source. Superposition theorem is a way to determine currents and voltages in a linear circuit that has multiple sources by taking one source at a time. the current in any given branch of a multiple-source linear
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