oil’s refractive properties. The oil works as a sort of secondary lens‚ magnifying the specimen in addition to the lens already present. 3. Describe the differences you observed between the cells seen on the blood slide and those seen on the bacterial slides. (1) They were very much so different. The yogurt cells were rod-like and fairly widely distributed. The blood cells on the other hand‚ were round and appeared in one giant cluster tightly packed together. 4. What is the difference between
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organisms. The processes involved in the isolation of these organisms include; culturing‚ staining‚ microscopy and sensitivity tests. Samples collected for examination include; stool‚ blood‚ sputum‚ urine‚ vaginal swab‚ wound swab and wound biopsy. Bacterial Culturing and sensitivity Cultures are carried out to isolate suspected organisms from a sample. There are different types of culture such as; blood culture‚ sputum culture‚ urine culture‚ wound swab and biopsy culture. Sputum culture: Required
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differential stain? How is it different from a simple stain? the use of two or more stains to categorize cells into groups. simple stain uses one dye and shows only structure‚ morphology and size. a differential stain uses two or more dyes and is used to show morphology and distinguish cell types B. What is the difference between Gram-positive and Gram-negative cell walls? Structure: Have thick layer of peptidoglycan over inner cytoplasmic membrane. lack
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precipitation of CaCO3. Bacterial cell walls and exopolymers exuded by bacteria serve as an important site for adsorption of ions‚ mineral nucleation and growth [1‚ 2].These biopolymers have been found to play important role in morphology as well as mineralogy of carbonate precipitates [3]. Kawaguchi and Decho [4] reported that matrix of extracellular polymeric secretions influence CaCO3 precipitation by providing a suitable matrix for precipitation by successive stratification on the bacterial
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microscopy‚ prepare and observe bacterial slides and perform staining methods and explain the mechanism of the bacteria. In order to observe and investigate microbes we need to use microscope and bacterial Staining Techniques. Microscope is the invaluable tool
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single organism. Gram’s Stain is a widely used method of staining bacteria as an aid to their identification. It was originally devised by Hans Christian Joachim Gram‚ a Danish physician. Gram’s Stain differentiates between two major cell wall types. Bacterial species with walls containing small amounts of peptidoglycan‚ and‚ characteristically‚ lipopolysaccharide‚ are gram negative whereas bacteria with cell wall containing relatively large amounts of peptidoglycan and no lipopolysaccharide are gram positive
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Abstract In an environment isolation procedure‚ experiments under categories‚ such as‚ morphology‚ physiology‚ antibacterial susceptibility‚ selective media‚ and biochemical provide results. Both the unknown isolate and members of the Micrococcus genus were shown to be obligate aerobes. By using staining methods‚ this proved that the organism is gram positive. Morphology‚ such as‚ orange pigmentation and coccus shape provide similarities to the Micrococcus genus. Physiological tests were shown to
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Laboratory Report: Observing Bacteria Purpose: Is to gain knowledge on how to use a microscope and oil immersion lens while observing prepared bacterial slides and also to prepare slides for observable bacteria culture from yogart. Procedure: Exercise 1: Using the Microscope - Viewing Prepared Slides I started out by reading and reviewed the science lab safety reinforcement Agreement and the instruction to oil immersion lens. After setting up my lab station I view the six prepared slides
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Introduction Gram staining was developed by Christian Gram in the 1800’s‚ a Danish bacteriologist. (Smith and Hussey‚ 2005) It was the first differential staining technique and most common used in microbiology. Furthermore‚ bacteria are transparent and cannot be seen through the microscope. For that reason‚ Gram staining is an important tool for distinguishing between two main types of bacteria Gram-positive and Gram-negative. The Gram stain differentiates the Gram positive and gram-negative on
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different environmental factors. Good aseptic techniques were used to prevent contamination‚ resulting in a uniform set of results that are in line with the literature. Introduction Bacteria vary greatly in terms of their characteristics and morphology. Colonies can be classified according to their colour‚ form‚ elevation‚ margin and size. Pure cultures of microorganisms are those that are uniform and are of the same descendants of the same organism. The isolation of colonies by streak plate technique
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