Harley−Prescott: Laboratory Exercises in Microbiology‚ Fifth Edition Front Matter Preface © The McGraw−Hill Companies‚ 2002 PREFACE Take interest‚ I implore you‚ in those sacred dwellings which one designates by the expressive term: laboratories. Demand that they be multiplied‚ that they be adorned. These are the temples of the future—temples of well-being and of happiness. There it is that humanity grows greater‚ stronger‚ better. Louis Pasteur (French chemist‚ founder of microbiology
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these stains: (Functions and Reagents) Simple Stain- CULTURES: Staphylococcus epidermis slant Bacillus megaterium broth MATERIALS USED: Methylene blue‚ Distilled water‚ Slide‚ Inoculating Loop FUNCTION: To observe shape size‚ morphology‚ and arrangement. ---FROM SOLID: (slant) 1. Mark the smears on the underside. 2. Place 1 to 2 loopfuls of water on the slide. 3. Transfer a small amount of culture with a sterile loop. (Mix with water) 4. Allow the smear to
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Lab 1 – Ubiquity of Microbes Bacteria are the most widely distributed organisms in the biosphere. They are found in all different types of soil‚ water and even in the bodies of other living organisms. Every single place that humans have explored on the Earth‚ have shown to contain microbes. Bacteria are living organisms and like all living organisms‚ they require energy and carbon sources in order to metabolize and reproduce. And like all living organisms they respond to changes in their environment
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Coccus is a term used to describe a type of morphology that helps us distinguish different kinds of bacteria. The cell’s morphology is only one of the many ways we can differentiate the different species. Looking at these bacterium underneath the microscope is an important step to determine which kind of species it is. All cocci bacteria are not gram positive‚ but this lab will examine only this kind because of their influence in human health. Staphylococcus Aureus is a gram-positive coccus bacterium
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Karyo = nucleus‚ pro= pre‚ Eu= true 3. Components of a cell: a. Nucleus: brain of cell; has nuclear membrane/envelope 1. DNA à Chromosomes (Genes) à make protein à Macromolecule. 2. Mitosis‚ Cell Division b. Nucleoid Material (Bacterial DNA; note: no nuclear membrane. 1. Essential Genes (instructions). 2.Nucleoid Material ONLY used for bacteria a. Single Chromosome i. Human chromosomes à ROD c. Protoplasm: Composed of Karyoplasm & Cytoplasm 1. Water w/ particles
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E. coli O157H7 What is the morphology and gram reaction of this pathogen (2) E. coli‚ including E. coli O157H7 is a gram-negative bacillus. What do (i) O157 and (ii) H7 attached to the name of this bacterium represent (2). The O157 is the O HYPERLINK http//www.emedicinehealth.com/script/main/art.asparticlekey5469 serotype antigen that identifies the E. coli strain‚ and the H7 represents the antigen type on the HYPERLINK http//www.emedicinehealth.com/script/main/art.asparticlekey2416 bacteriums
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great potential to degrade diesel engine oil i.e. 89.98 % after 25 days. The rate of degradation of diesel engine oil by Micrococcus sp. was found to be 7.48 x 10-4gm/hr and that of Pseudomonas sp. was 9.55 x 10-4gm/hr while the mixture of both bacterial isolates showed highest rate of degradation of diesel engine oil i.e. 1.27 x 10-3gm/hr. Keywords: Bioremediation‚ Diesel engine oil‚ oil spills‚ hydrocarbon degraders‚ Micrococcus sp.‚ Pseudomonas sp. Introduction As we dig deeper into the modern
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drops of safranin to the slide and let sit for at least 1 min. 7.) Wash off safranin and then carefully blot the slide dry with a paper towel. 8.) Observe the bacteria through a microscope under the oil emersion lens. 9.) Be sure to note the morphology of the bacteria and whether it is gram-positive or negative. (Hold on to bacteria tube for future tests) Procedures 2: (Tests performed based upon the results from gram-stain). 1.) Next obtain 1 sheep blood agar plate and inoculate it
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bacteria were single-cell microorganisms that were the first forms of life to appear on Earth‚ about 4 billion years ago. For about 3 billion years‚ all organisms were microscopic‚ and bacteria and archaea were the dominant forms of life. Although bacterial fossils exist‚ their lack of distinctive
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SANDRA-EMMANUELLA 12CQ014231 OLOWOFELA OLUWATOYIN 12CQ014232 OSITA-ORAH ANTHONY 12CQ014233 SASORE YEWANDE 12CQO14236 NWOBI CHIZITERE 12CQ014229 OYEKAN TOSIN 12CQ014235 SOREMEKUN MAYOMIKIUN 12CQ014238 SHODEKO TOBI 12CQ014237 INTRODUCTION TO THE BACTERIAL CELL (EUBACTERIA) Bacteria (singular: bacterium) constitute a large domain of prokaryotic microorganisms. Typically a few micrometres in length‚ bacteria have a wide range of shapes‚ ranging from spheres to rods and spirals. Bacteria were among
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