study and characterize a certain microorganism‚ microbiologists need to separate and isolate this microorganism from the many other microorganisms with which it usually shares its natural environment or habitat; proper and professional execution of bacterial isolation techniques are at the centre of routine activities in any professional microbiology laboratory. The task of the microbiologist is to isolate the microorganism under investigation from the mixed culture of the sample and to create a pure
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Describe the four main bacterial shapes. Cocci – oval or spherical shaped. Bacillus – are rod shaped. Spirillum – are thick‚ rigid spirals. Vibrio – are curved or a comma shaped rod. D. What are the common arrangements of bacteria? Diplo – oval shaped‚ found in pairs. Strepto – are cocci that arange into chains. Staphylo – are cocci that are arranged into irregular clusters‚ similar to grapes. E. Were you able to identify specific bacterial morphologies on either yogurt slide? If
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have explained their role in the solubilization process by identification and quantification of these OAKhan et al. 2010; Marra et al.( 2012 ) In natural conditions or under in vitro conditions OA produced by many P-solubilizers‚ for example‚ bacterial cultures or fungi ‚ chelate mineral ions or lowers the pH to bring P into solution. ((Pradhan and Shukla 2005 Khan et
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Microbiology! 1) Groups of Organisms (7): Eukaryote: TRUE nucleus 1. Fungi 4. Bacteria 2. Algae 5. Rickettsia 3. Protozoa ! Prokaryote: NO Nucleus Ribosome is the only Organelle 6. Mycoplasma (7. Viruses: No cell structure‚ ARE NOT CELLS) Microorganisms are: ! UBIQUITOUS! ! HARMLESS IN THEIR NORMAL HABITAT ! OPPORTUNISTIC (If they leave their normal habitat and get into us) 2) Roles of Microorganisms: (Bacteria and Fungi are “Semi-Good”) - digestion -
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as gram insensitive. I hypothesize that in the cultures used in this lab for gram staining will contain many gram negative and gram positive bacteria. Material and Methods The following things are needed to run a gram staining experiment: 1. Bacterial cultures 2. Glass slides 3. Cover slips 4. Ammonium Oxalate-Crystal Violet stain 5. Gram’s Iodine Solution 6. Ethyl Alcohol 7. Safranin Solution 8. Paper towels 9. Water 10. Bunsen Burner (Lighter will suffice) 11. Microscope 1. Prepare
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gyMicrobiology: is a study of microorganisms (microbes‚ germs) and their activities Microorganism: An organism or a virus too small to be seen without a microscope (smaller than 0.5mm) Misconceptions of Microorganisms * All microorganisms are pathogenic (disease producing) FALSE * The majority of bacteria are harmless and beneficial TRUE Aspects of microbiology * Basic biological science * About living cell and how they work * About microbial diversity and their evolution
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were inoculated to SBAP 2 in order to isolate the tan colonies without being mixed with the other colony. In SBAP 2‚ the split plate of γ-hemolytic colonies and tan colonies from the CHOC. After incubation‚ the tan colonies showed irregular colony morphology which has a formation of halo clearing on the sides of the
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Gram negative bacteria stain red‚ because it has a thinner peptidoglycan wall and does not retain the crystal violet during the de-coloring process. The gram staining technique was also used to form distinctive cell morphologies because cell shape is generally specific to bacterial species. (Tortora‚ Funke) The next test that was performed was the Carbohydrate fermentation broth test. The reason for doing this test was to identify the bacteria by determining what nutrients they can utilize and
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Question 1 5 out of 5 points Consider the following three common agents of bacterial meningitis: Neisseria meningitidis‚ Streptococcus pneumoniae‚ and Haemophilus influenzae B. Which of the following would be most helpful in differentiating these three agents? Answer Selected Answer: gram-stain morphology Question 2 5 out of 5 points The current first choice of antibiotic for bacterial meningitis is ________. Answer Selected Answer: cephalosporin Question 3 5 out of 5 points
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was performed. In section 10‚ Figures‚ there are some pictures corresponding to the stains performed. In Figure 1‚ the Gram-positive stain‚ you can clearly see the purple color and cocci morphology in grapelike clusters. Figure 2 is the Gram-negative stain with the safranin color and shows bacilli cellular morphology. Gram-positive sample Test Utilized Result Controls Gram Stain Gram-positive cocci Positive-S. epidermidis Negative- E. coli Hemolysis Test Full clearing of agar‚ Beta- Hemolysis
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