Identification of A Mixed Culture Unknown An experiment such as this one serves the purpose of allowing us‚ the students‚ to apply what we already know about any organism and any laboratory procedure to the difficult task at hand. It is possible to identify a mixed culture by running familiar experiments on the unknown bacteria and taking information already known about specific bacteria and applying it to the results. This helps to slowly eliminate any bacteria that do not correspond with the
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Botulism Botulism is a bacterial disease most often linked to un-sterile canned foods. It can be rather serious‚ as it has the potential to paralyze the respiratory system and thus cause death. The bacterium is named Clostridium Botulinum‚ it is a gram-positive and anaerobic pathogen. The morphology consists of rod shaped spores. The toxin is widely used in cosmetic injections‚ also known as Botox‚ to decrease wrinkles in the skin. Though Botulism is mainly linked to canned foods
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present. I am doing his experiment to verify the concept that microorganisms are everywhere and I most certainly expect my results to prove so. I expect to find many shapes‚ sizes‚ colours and indeed types of microorganisms present when I observe the morphology of the colonies present. Materials and methods: Swabs. Diluent 0.1% peptone. TSA (Trypticase
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UNIT 9: VIRUSES‚ VIROIDS‚ & PRIONS Lesson 1 – Characteristics of Viruses Depending on their characteristics‚ viruses may or may not kill the host cell. Viruses are too small to be seen with a light microscope and cannot be cultured outside their hosts. Viruses and Bacteria Compared. | Bacteria | Viruses | | Typical Bacteria | Rickettsias/Chlamydias | | Intracellular Parasite | No | Yes | Yes | Plasma Membrane | Yes | Yes | No | Binary Fission | Yes | Yes | No | Pass through
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Dispose of used disposable cups and swabs in the garbage. 9) Disinfect work area with bleach‚ wash hands thoroughly with soap and water. The remaining supplies can be returned to the lab kit for future use. Part 2 1) Examine agar plate for bacterial growth. It there is no colony growth‚ incubate an additional 24 hours. Be very careful to not remove the lids. 2) Prior to beginning part 2‚ disinfect the work area with bleach and paper towels. Then‚ wash hands with soap and water‚ put on the
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Use of differential‚ selective and enriched media: EMB‚ blood and starch agar. OBJECTIVES: distinguish between different bacterial species based on colony morphology on agar plates To distinguish the growth characteristics of microorganisms in various differential‚ and selective media. Differentiate bacteria based on their ability to hydrolyze starch. Materials: Plates of EMB‚ Starch and blood agar. Stool sample. Inoculating loop. Bunsen burner. Soil sample. Cotton soap. Skin
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When conducting the gram staining test there was of evidence to support the initial hypothesis of the effect of the bacterial cell with the addition of Gram’s alcohol as the the lipopolysaccharide layer was removed in Culture A and in Culture B there was in observance of a shrinkage in pore
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Lab Report #1: Observing Bacteria Microbiology Abstract: This lab exercise familiarized the student with the use of a microscope by observing and identifying various different slides under the microscope. The student practiced observing the given slides under the 10x‚ 40x‚ and 100x (oil immersion) objective lenses‚ which allowed for the identification of the different organism’s shapes and sizes. Purpose: The aim of this exercise is to equip the student with the knowledge
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Publishers. 4. Cullimore‚ D.R. (2000). Practical Atlas For Bacterial Identification. Boca Raton‚ Florida:
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quadrant to the second using the quadrant streaking method as illustrated on page 18 of the lab manual‚ repeating this process until all four quadrants were properly streaked. Lab day 2; we collected our BHIA medium and began by identifying the morphology and cell-to cell arrangements of the colonies. Two different colonies were observed‚ the first colony was yellow in color and larger in size and the white colored colony was slightly smaller in size. As instructed‚ each colony was prepared for
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