Bacterial growth patterns and the effects of environmental factors on properties of colonies ERIC LI Abstract In this experiment we work with different types of bacteria and experiment with their different properties and how they grow in certain situations. Different samples of bacteria cultures were gathered from different places such as the mouth‚ shaded mulch‚ and from the table top. The samples were collected by using a cotton swab and swiped onto a petri dish filled with nutrient agar. The
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Bacterial Genetics Worksheet 1. Fill in: Transformation Transduction Conjugation a. Transformation _ naked DNA is involved b. Transduction ____faulty head stuffing may occur c. Conjugation_____ involves exconjugants d. Transduction____ adsorption and injection of genetic material e. Transformation__ competent cells required f. Conjugation ____ an F plasmid encodes attachment proteins g. Conjugation _____used to construct the E. coli minute map h. Conjugation_____
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Practical 9: Examination of Bacterial Plates Objective: 1. To examine bacteria pigmentation‚ colony‚ margin characteristics‚ elevation properties‚ broth characteristics and agar stroke properties. 2. To examine bacteria growth characteristics on different culture media. Introduction: Bacterial species can sometimes be identified on the basis of how they appear on or in the different media. The pigmentation‚ size and shape of bacterial colonies as they grow on and in agar plates can
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Ensuring the Social‚ Political‚ and Technological Protection of Water Quality Protecting Against Bacterial‚ Parasitic‚ and Protozoan Pathogens Nathan Pankowsky Contents Abstract....................................................................................................................................................... 2 Introduction ............................................................................................................................................... 3
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Bacterial Transformation Lab Introduction: In this experiment we transformed a strain of E. Coli bacteria without antibiotic resistance with plasmid DNA. This plasmid produces a fluorescent green glow under black light due to the gfp(green fluorescent protein) as well as antibiotic resistance. E. Coli cells will be plated on an agar medium‚ some with and some without the antibiotic ampicillin. Only bacterial cells that contain the plasmid will survive the ampicillin and produce the green glow
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Chliamydia trachomotisDirect contact (fomites‚ flies) Conjunctivitis #1 cause of blindness in world Bacterial Meningitis Haemophilus influenza type B Direct Contact Endogenous infection: aerosols Fever‚ headache‚ stiff neck‚ nausea‚ vomiting Rare in U.S. Bacterial Meningococcal Meningitis Neisseria meningitidisDirect Contact Droplet Transmission (Aerosols) Petechial rash Global problem Bacterial Pneumococcal Meningitis Streptococcus PneumoniaeDirect Contact Droplet Transmission Aerosols
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Unit 3. Assignment 1: Bacterial Growth Rates 1. Mediums that could be used to determine shigellosis include Btilliant Green Agar‚ and Triple Sugar-Iron Agar. Expected results in a confirmed case of shigellosis are as follows: Brilliant Green Agar – Isolated Shigella colonies which do not ferment lactose or sucrose and appear red or white in color with no growth to trace growth on the Agar plate will be present. Triple Sugar-Iron Agar – Presence of Shigella will manifest as a red slant with
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Bacterial Growth Requirements Evelyn Lyle ITT Technical Institute Angela Ask‚ MPS January 15‚ 2012 Every organism must find in its environment all of the substances required for energy generation and cellular biosynthesis. The chemicals and elements of this environment that are utilized for bacterial growth are referred to as nutrients. Many bacteria can be identified in the environment by inspection or using genetic techniques. The nutritional requirements of a bacterium such as E Coli are
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Bacterial growth is the division of one bacterium into two daughter cells in a process called binary fission. Providing no mutational event occurs the resulting daughter cells are genetically identical to the original cell. Hence‚ "local doubling" of the bacterial population occurs. Both daughter cells from the division do not necessarily survive. However‚ if the number surviving exceeds unity on average‚ the bacterial population undergoes exponential growth. The measurement of an exponential bacterial
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Modeling bacterial growth is important in maximizing the efficiencies of biological processes. Although there are many different methods of modeling bacterial growth‚ this experiment focuses on the Monod equations. However‚ in order to use the Monod equations‚ the maximum growth rate and Monod constant must be found. Here we show how the maximum growth rate and Monod constants can be obtained for Escherichia coli using M9 media in a bioreactor at 37 °C and 500 RPM. The maximum growth rate is also
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