LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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MBK – Lab Report Name: ____________________ Section: ___________________ Bacterial Morphology Pg 61 Part 1: Viewing Prepared Slides of Common Bacterial Shapes Familiarize yourself with each morphological type to use as a comparative tool for the remainder of the activity. Record your observations. Part 2: Disinfecting Your Area to Use Live Organisms: Part 3: Viewing Live Organisms – Wet Mount Preparation Record your observations. Part 4: Direct Staining:
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encoded by EZH2‚ the EZH2 gene encodes part of the Polycomb group which make protein complexes that help to maintain genes transcriptional repressive state over successive cell generations. http://www.ncbi.nlm.nih.gov/gene/2146 Throughout this lab report template DNA that contains the gene EZH2 was provided‚ this will be amplified by a PCR and cloned into a vector. This Polycomb group proteins help maintain the cell identity during progress through chromatin
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chemicals before use. Abide by specific warnings and directions. 3. Collect all materials needed for a procedure before proceeding. 4. Perform reactions under the hood when directed. Chemicals may be weighed and prepared at balance or lab tables‚ but tests should be carried out under the hood. 5.Acids and caustic chemicals are stored in the hood. Please do not take these chemicals from the hood. Procedure: PART 1: Metathetical reactions Precipitation reactions A1. Add a
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Organic Chemistry II Lab 9 Fermentation of a Carbohydrate: Ethanol from Sucrose * Introduction Ethanol is one of the oldest alcohols and also the least toxic one. Industrially‚ ethanol is made most economically by hydration of ethylene. However‚ ethanol that is intended for human consumption must‚ by law‚ be prepared by fermentation. By either method‚ ethanol‚ of course‚ has the same formula‚ structure‚ and properties. The fermentation takes place with the assistance of enzymes from yeast
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substances according to specific body needs. The process of urine formation and adjustment of blood composition involves three processes: glomerular filtration‚ tubular reabsorption‚ and tubular secretion. The first part of the process of urine formation occurs in the glomeruli which act as filters‚ allowing water‚ glucose‚ salt and waste materials to pass through to the Bowman’s capsule but preventing and red blood cells and plasma proteins to pass through. Reabsorption occurs in the proximal tubules of
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Observing Argumentation In the 21st century‚ people are very set in the stereotypical thoughts that society implants in their heads. Many people don’t like to step out of their comfort zone when it comes to values and stereotypes. In both stories‚ I personally think the theme is that everyone should step out of the thoughts that they are used to and be more open-minded about the world. In Fatema Mernissi’s article “Digital Scheherazade; The Rise of Women as Key Players in the Arab Gulf Communication
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Antibiotic Lab Report By: Sierra Toll Hour: 6 Title: The effects of antibiotics on the growth of Escherichia Coli Problem: How do antibiotics affect the growth of E coli? Hypothesis: If Penicillin is applied to the bacteria‚ then the zone of inhibition will be larger. Background: The purpose of this lab was to see how effective a certain antibiotic is to kill E coli. The lab tested three antibiotics‚ Penicillin‚ Erythromycin‚ and Neomycin. The zone of inhibition‚ which is the amount of space
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can survive in extreme environments. Halobacteria are also useful by being a good organism to perform DNA transcription‚ translation‚ and transformation on (Kramer‚ 2006). There are two different types of Halobacteria that are being observed in this lab. The first is NRC-1‚ which is also called the wild type strain. Although the pigmentation of the Halobacteria is caused by the production of the membrane protein‚ bacteriorhodopsin‚ which is a red‚ the wild type strain is pink in color. This pink color
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BACK TITRATION- DETERMINATION OF THE CARBONATE CONTENT IN GARDEN LIME NAME: OSEI BONSU ERIC ID: 3906409 EXPERIMENT: I.2.2.1.
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