Genetic transformation of Escherichia coli with pGLO (Adapted from: Biotechnology Explorer: Bacterial Transformation: The pGLO System. Instructors Guide. BIO-RAD). Objectives a. To understand one of the most commonly used techniques for introducing DNA into E. coli cells and its use in molecular cloning. b. To become familiar with the concept of using green fluorescent protein (GFP) as a molecular tag for studying gene expression in bacteria and other organisms.
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October 13‚ 2012 Needle through a Balloon Pre-Lab Preparations: Purpose: The purpose of this experiment is to see if the balloon pops when pierced with a wooden skewer or a sharp pin. Hypothesis: If we lubricate the skewer with petroleum jelly‚ then it should go through the balloon without popping. Hypothesis for the 2nd part of the Lab: If we cover the side of the balloon with cellophane tape‚ then it will not pop when pierced with the pin. Materials: • Balloons • Long wooden
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No man thinks more highly than I do of the patriotism‚ as well as abilities‚ of the very worthy gentlemen who have just addressed the house. But different men often see the same subject in different lights; and‚ therefore‚ I hope it will not be thought disrespectful to those gentlemen if‚ entertaining as I do opinions of a character very opposite to theirs‚ I shall speak forth my sentiments freely and without reserve. This is no time for ceremony. The question before the house is one of awful moment
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LAB 14 REMOTE WINDOWS 7 ADMINISTRATION This lab contains the following exercises and activities: Exercise 14.1 Creating a Custom MMC Console Exercise 14.2 Configuring Remote Desktop Access Exercise 14.3 Connecting to a Remote Workstation Exercise 14.4 Creating an RDP File Lab Challenge 14.1 Using Windows Remote Management SCENARIO You are a newly hired desktop technician for Contoso‚ Ltd.‚ working on a long- term test deployment of new Windows 7 workstations. You have been given the
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Introduction The purpose of this lab was to recognize the stages as well as events of mitosis and meiosis in a plant cell or an animal cell. The relative duration of each stage in the cell cycle will also be learned during this lab. By doing this lab we will also create a model of and understand the process of crossing over. This lab also helps students understand the similarities along with differences between mitosis and meiosis. Purpose The objectives of the lab were to help give us a better
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Lab Report for Chemical Changes and Conservation of Mass Lab Previous to the reaction‚ the magnesium appeared to be a solid‚ white‚ black‚ and grey substance with many small dark particles within the substance. The hydrochloric acid was a clear liquid that looked like water It weighed 42.2 grams in total. During the reaction‚ the two substances produced bubbles‚ creating smoke and became warm with a smell that is irritating to the human nose. After the reaction‚ it appeared to be a clear‚ bubbly
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Review of Me and You and Everyone We Know Miranda July’s independent movie Me and You and Everyone We Know is very cute and a little bit bizarrely funny‚ which mainly observes of how modern people struggle to connect with each other in an isolating world. Through the usage of film techniques such color and the camera distances and angles‚ views get a better understanding of how the central character Christine‚ a lonely artist who operates a cab service for the elderly‚ falls in love and holds hands
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A. What is the name of the pigment that captures light? Chlorophyll B. Why does the pigment appear green? It absorbs other colors of light such as red and blue‚ so in a way the green light is reflected out since the pigment does not absorb it. 2. List two variables besides the wavelength (color) of light which might affect the rate of food production in plants. Light intensity and carbon dioxide 3. Why is chlorophyll important for all biological life? It is necessary for photosynthesis which
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Environmental Health Sciences Center Community Outreach & Education Program CHROMATOGRAPHY (Adapted from: Forensic Science Activities. University of Colorado Boulder Hughes Initiative. .) DESCRIPTION: Students will use paper chromatography to separate ink molecules and identify the pen used on an unknown sample of handwriting. Students will graph and analyze data they collect using paper chromatography. PURPOSE/GOAL: Students will be able to: • Gain understanding of the purpose of chromatography
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Procedures: 1) Measure out 30 yards of space‚ have of member stand at one end of the field with a stop watch in hand. 2) Another member throw the tennis ball 30 yards while timing how long it take to get there. 3) Repeat 2 more times and collect the average speed (total distance/ total time). 4) Repeat this process using the racket ball. Record in table 1. 5) For experiment 2‚ measure one of the steps and multiply by how many steps you have. 6) Have a member at the bottom of the steps with a
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