Damage of Beet Cell Membranes Caused by Different Temperatures Resulting in Betacyanin Leakage By: Trevor Sandum‚ Lab 2‚ March 9‚ 2013 Introduction: A red pigment called Betacyanin located in the vacuole of the beet cells is released when the membrane is damaged (Danyk‚ 2013). The membrane is necessary for sustainability of the cell’s life‚ it separates the cell from the outside environment‚ and is responsible for diffusion‚ exocytosis and endocytosis‚ and transport. It
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Rational Mutational Analysis of a Multidrug MFS Transporter CaMdr1p of Candida albicans by Employing a Membrane Environment Based Computational Approach Khyati Kapoor1.‚ Mohd Rehan2.‚ Ajeeta Kaushiki2‚ Ritu Pasrija1¤‚ Andrew M. Lynn2*‚ Rajendra Prasad1* 1 School of Life Sciences‚ Jawaharlal Nehru University‚ New Delhi‚ India‚ 2 School of Information Technology‚ Jawaharlal Nehru University‚ New Delhi‚ India Abstract CaMdr1p is a multidrug MFS transporter of pathogenic Candida albicans. An over-expression
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Aim: Investigate the effect of different temperatures and chemicals including various pH levels‚ alcohol and detergent on the cell membrane structure as well as function. Hypothesis: Increasing the temperature and pH levels which the beetroot membrane cells are exposed to will increase the amount of dye that passes out of the membrane. Materials: Part A • Bunsen Burner‚ Tripod and a Wire Gauze • 250mL Beaker • A 100° thermometer • Matches • 10mL measuring cylinder • Sticky labels and a Pen
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regent (I2Kl) solution on the outside of the beaker permeates the intestine (Dialysis tubing) inducing a chemical reaction that will turn the liquid in the intestine (Dialysis tubing) blue-black. If the amylase successfully digests the starch‚ what results would you expect? If the amylase successfully digests the starch‚ I would expect a no color change occur as well as a presence of glucose in the intestine (Dialysis tubing). If the amylase only partly digests the starch‚ what would you expect
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Title: Colligative Properties & Osmotic Pressure Background: Important terms to study from this lab assignment are colligative properties‚ membrane permeability and osmotic pressure. First‚ colligative properties are “those of a solution that depend solely on the number of solute particles present‚ not the identity of those solute particles. These properties include: vapor pressure lowering‚ boiling point elevation‚ freezing point depression‚ and osmotic pressure” (p. 17 lab manual). In this experiment
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lab topic‚ you should be able to: 1. Describe the mechanism of diffusion at the molecular level. 2. List several factors that influence the rate of diffusion. 3. Explain why diffusion is important to cells. 4. Describe a selectively permeable membrane‚ and explain its role in osmosis. 5. Define hypotonic‚ hypertonic‚ and isotonic in terms of relative concentrations of osmotically active substances. 6. Discuss the influence of the cell wall on osmotic behavior in cells. 7. Explain how incubation
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semi‐permeable membrane (dialysis tubing) and sucrose will create an osmotic environment similar to that of a cell. Using different concentrations of sucrose (which is unable to cross the membrane) will allow us to examine the net movement of water across the membrane. INTRODUCTION A major determinant of diffusion in a biological system is membrane permeability. Small‚ uncharged molecules pass through cellular membranes easily‚ while most and/or charged molecules cannot pass through the membrane. The
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soaked dialysis tubing * 2 dialysis tubing clamps or pieces of twine * 2 clean plastic pipettes * 1 bottle of Lugol’s solution * 2 glucose test strips Procedure: Begin the experiment by placing 4 full pipettes worth of cooked starch in a beaker. Then‚ use a second clean pipette to add 4 full pipettes worth of α-amylase to the beaker and stir to simulate food mixing with saliva in the mouth. Afterwards‚ leaving the mixture in the beaker‚ obtain a piece of soaked dialysis tubing and
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since the weight of the potato did not change after it was incubated in the solution with 0.4M. We also found that the potato was hypertonic to sucrose solutions of 0.0-0.3M and hypotonic to 0.5-0.6M. C. Dialysis Tubing Experiment We tested the permeability of the dialysis tubing to glucose‚ starch‚ and iodine. Initially‚ glucose and starch were placed into the bag‚ and iodine outside of the bag in the beaker. We used the iodine test for the presence of starch which proved positive inside
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Diffusion is the movement of particles from an area of higher concentration to an area of lower concentration. Because of the selective permeability of the membrane only water and other very small particles (iodine) can be diffused through simple diffusion. The solution out of the dialysis tubing had a higher distelled iodine concentration of solutes (iodine + H2O) than did the starch solution. So iodine move into the cell and react with starch molecules to formed starchiodine‚ the blue compound
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