________________________ A C T I V I T Y 1 1 Cell Transport Mechanisms and Permeability Simulating Dialysis (Simple Diffusion) 1. Describe two variables that affect the rate of diffusion. ______________________________________________________ __________________________________________________________________________________________________ 2. Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? ______________
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REVIEW SHEET EXERCISE 1 Cell Transport Mechanisms and Permeability Simple Diffusion The following refer to Activity 1: Simulating Dialysis (Simple Diffusion). Which solute(s) were able to pass through the 20 MWCO membrane? NONE 1. According to your results‚ which solute had the highest molecular weight? Albumin 2. Which solute displayed the highest rate of diffusion through the 200 MWCO membrane? Na+Cl 3. Using the data from Chart 1‚ explain the relationship between the
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PURIFICATION OF LACTATE DEHYDROGENASE FROM CHICKEN MUSCLE TISSUE Abstract The enzyme lactate dehydrogenase (LDH) catalyzes the last step of anaerobic glycolysis that is important for the normal function of the body. Purification of LDH is essential to understand its structure and function. The purpose of this experiment was to extract and purify LDH enzyme from chicken muscle tissue using a variety of various. Analytical methods such as activity and protein assay were employed to determine
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G. Y. S. Period: 6 09/11/12 Osmosis Lab I. Objective: The purpose of this lab is to put the solutions in chronological order from least to most molarity. If the solutions are hypertonic then‚ the water will go in and the order of the substances will be C‚ D‚ A‚ B‚ E. II. Materials and Methods: See attached page. Results: Table 1.1 III. Table 1.1 | Initial | Final | % Change | A | 11.4 | 11.86 | 4.03% | B | 11.67 | 15.33 | 33.68% | C | 10.84 | 11.86 | 9.4% | D | 12.02 |
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metabolism from the body”. Ingestion: “The intake of substances into the stomach”. Digestion: “The breakdown of molecules that are ingested from large molecules into small molecules”. Absorption: “The intake of those small molecules through the plasma membrane and into the cells”. Egestion: “The removal of waste products not used in the metabolism e.g. Cellulose & faeces”. Metabolism: “All ongoing chemical and biological reactions in the body”. Organs of excretion: Skin: Salt and water in sweat
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Lab Manual—Version 1.1 Introductory Anatomy & Physiology © 2012‚ eScience Labs‚ LLC. All Rights Reserved www.esciencelabs.com • 888.375.5487 Table of Contents Anatomy & Physiology Version 1 Preface: Introduc on to the Fetal Pig Lab 1: The Key to Reproducible Science Lab 2: Cell Structure and Func on Lab 3: Mitosis and Meiosis Lab 4: Diffusion and Osmosis Lab 5: Tissues and Skin Lab 6: The Skeletal System Lab 7: The Muscular System Lab 8: The Nervous System Appendix: Good Lab Techniques
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molecules can move across a cell membrane by diffusion. OSMOSIS= Diffusion of fluid through a semipermeable membrane from a solution with a low solute concentration to a solution with a higher solute concentration until there is an equal concentration of fluid on both sides of the membrane. SELECTIVE PERMEABLE MEMBRANE=a membrane (as a cell membrane) that allows some molecules to pass through but not others cell membrane‚ cyto-membrane‚ plasma membrane - a thin membrane (a double layer of lipids) enclosing
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1 Cell Transport Mechanisms and Permeability Name ____________________________________________________________ Lab Time/Date ______________________________ Activity 1 Simulating Dialysis (Simple Diffusion) 1. Describe two variables that affect the rate of diffusion. * The size of the pores of the membrane * The size of the molecule diffusing through the membrane 2. Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare
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▪Secondary glomerular diseases that can have systemic effects include lupus erymatosus‚ Goodpasture’s syndrome (caused by antibodies to the glomerular basement membrane)‚ diabetic glomerulosclerosis and amyloidosis. PATHOPHYSIOLOGY ▪Kidneys are reduced to as little as one-fifth their normal size (consisting largely of fibrous tissue). ▪The cortex layer shrinks to 1-2mm in thickness or less. ▪Bands of scar
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and Permeability Name: Gayane koshkakaryan Lab Time/Date: Friday 8am Activity 1 Simulating Dialysis (Simple Diffusion) 1. Describe two variables that affect the rate of diffusion. a. Molecular weight b. Solute concentration 2. Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? a. Urea was unable to diffuse through the 20MWCO membrane because urea is a big molecule and the 20MWCO membrane pore
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