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    and methods Hosts ‚ plasmids and chemicals E. coli TOP10 strain was used for cloning and proliferation. E.coli BL21 DE3 was used as expression host cell. The pET 28a(+) plasmid was employed for gene expression. All chemicals were obtained from Merck Company (Germany). Codon optimization and gene synthesis Sequence encoding V-domain was obtained from Swiss-port‚ Uniprot KB and National Center for Biotechnology Information (NCBI) databases. 6x His-tag sequence was placed at N-terminal followed

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    Thin Layer Chromatography

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    Date:7.6.2013 Objectives: Part 1: (i)To learn the technique of TLC and the visualization of colourless components. (ii)Identify the unknown drug by a TCL comparison with standard components. Part2 :to learn the separation technique by using TLC plate in separating a mixture of compound by using spinach leaf. Apparatus:UV lamp‚capillary ‚250 ML beaker Materials:aspirin‚acetaminophen‚caffeine‚unknown A‚unknown B‚TLC plates‚ethyl acetate‚Hexane‚acetic acid‚iodine Introduction: Procedure:

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    How to Write Mission Statements Summing up your business’s mission helps you focus on the steps you need to take to succeed. Here’s how to create a mission statement that’s uniquely yours. A mission statement is a key tool that can be as important as your business plan. It captures‚ in a few succinct sentences‚ the essence of your business’s goals and the philosophies underlying them. Equally important‚ the mission statement signals what your business is all about to your customers‚ employees‚

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    dedicated to developing products that will provide excellent health benefits. This is rare because there are only a handful of large‚ research-based pharmaceutical companies willing to put such a large amount of annual sales into R & D. Merck‚ a research-based company‚ put almost 20% of their sales into R & D but Bristol-Myers Squibb’s other competitors like Johnson & Johnson and Proctor and Gamble Pharmaceuticals put only 1.3% and 2.6% of annual sales into R & D‚ respectively. This is

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    Soil Sample in Cr

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    nevertheless knowledge of how farms developed good crops; if I can do it anyone can with the proper material. If a farmer is given or has access to an Eijkelkamp Edelman 120 cm soil auger‚ Munsell soil color chart‚ Suunto clinometers‚ clear plastic tubes‚ Merck Universalindikator pH 0-14 strips‚ Map of Costa Rica’s life zones‚ and tape measure‚ they will be on a great path. With these materials and the proper orientation‚ any ordinary farmer can carry out his or her own experiment of the soil effectiveness

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    for the family members and they must be supervised responsibly. In addition‚ he explained that money has been overrated; for this‚ it pushes individuals into medical depression. Jon Baumhauer‚ a psychologist and Chairman of the Family Board of the Merck Family‚ explains that the money invested in a family business is so special‚ and advises: “The family’s values are the same as the values of the company.” Moreover‚ respect is the main value of the values that Baumhauer talked

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    Codes of Ethics

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    INTERNATIONAL BUSINESS ETHICS REVIEW A PUBLICATION OF THE INTERNATIONAL BUSINESS ETHICS INSTITUTE Creating an Effective Electronic Code of Conduct By Roland Riebl Companies increasingly distribute their codes of conduct to their stakeholders online to make the document more accessible and‚ perhaps‚ to overcome the traditional complexities associated with print codes. Additionally‚ the Sarbanes-Oxley act of 2002 requires publicly traded companies to make their codes available to the public. This

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    Shared with permission. ↑ Andrew Jose‚ Love Your Hair‚ p. 120‚ (2002)‚ ISBN 0-00-711900-3 ↑ Andrew Jose‚ Love Your Hair‚ p. 120‚ (2002)‚ ISBN 0-00-711900-3 ↑ The Merck Manual of Medical Information‚ Hair Loss‚ p. 1212‚ (2003)‚ ISBN 978-0-7434-7733-8 ↑ Winnie Yu‚ What to eat for what ails you‚ p. 158‚ (2007)‚ ISBN 978-1-59233-236-6 ↑ The Merck Manual of Medical Information‚ Hair Loss‚ p. 1212‚ (2003)‚ ISBN 978-0-7434-7733-8 ↑ Videojug‚ Hair loss basics‚ http://www.videojug.com/interview/hair-loss-basics

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    Business Ethics 101 Week 1

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    Business Ethics Week 1 Introduction to the Module INTRODUCTION THOUGHTS TO PONDER: • ‘A business that makes nothing but money is a poor kind of business’ (Henry Ford‚ US industrialist 1863-1947) • ‘The one and only social responsibility of business is to make profits’ (the economics guru Milton Friedman) The subject of ethics forms a central element in managerial responses to social forces. In many situations‚ an individual must reach a personal decision in regard to

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    Presentation1

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    UNIVERSITY OF CAMBODIA COLLEGE OF MANAGEMENT Business Ethics Chapter 5: Corporate Social Responsibility Lecturer: Ty Sopheavy Presenter: Hang Piseth Objective of this presentation: At the end of this presentation‚ you should be able to identify and describe: 1. Corporate social responsibility 2. Level of responsibility 3. Model of responsibility 4. The economic model of corporate social responsibility 5. The philanthropic model of corporate social responsibility 6. The social web model of corporate

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