Genetic transformation of Escherichia coli with pGLO (Adapted from: Biotechnology Explorer: Bacterial Transformation: The pGLO System. Instructors Guide. BIO-RAD). Objectives a. To understand one of the most commonly used techniques for introducing DNA into E. coli cells and its use in molecular cloning. b. To become familiar with the concept of using green fluorescent protein (GFP) as a molecular tag for studying gene expression in bacteria and other organisms.
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******************************************************************************************** Answer Sheet—Module 7 Lab DNA Extraction Click on the following link and view the DNA extraction: http://learn.genetics.utah.edu/content/labs/extraction/ 1. What is the source of the cells used in this demonstration? A human. 2. Give three practical uses of DNA that is extracted: a. Genetic testing b. Body Identification c. Forensic analysis 3. Name the piece of equipment
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primary judge stated the question at [1]‚ as whether a patent may be granted for a claim that covers naturally occurring nucleic acid – either DNA or RNA – that has been “isolated”. His Honour said‚ that in this context‚ the word “isolated” implies that the naturally occurring nucleic acid found in the cells of the human body‚ whether it be DNA or RNA‚ has been removed from the cellular environment in which it naturally exists and separated from other cellular components also found
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As humans‚ we are the most dominant species in the world. We have the ability to walk upright‚ grasping thumbs‚ and large brains. This helps us to live and be successful on earth. But‚ these advantages we have didn’t happen overnight‚ they occurred during the hominine evolution. The skull‚ neck‚ spiral column‚ hip bones‚ and leg bones of the early hominine species changed shape in ways that later enabled species to walk upright. The evolution of dipedal‚ or two-footed‚ locomotion was very important
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DNA DNA‚ or Deoxyribonucleic Acid‚ is described‚ in Encarta Encyclopedia as a genetic material of all cellular organisms and most viruses. DNA carries the information needed to direct protein synthesis and replication. Protein synthesis is the production of the proteins needed by the cell or virus for its activities and development. Replication is the process by which DNA copies itself for each descendant cell or virus‚ passing on the information needed for protein synthesis. In most cellular
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Unit 3 Lecture Study Guide 1. List the 4 primary tissue types and give the general characteristics and functions of each one. Epithelium- The epithelium protects us from the outside world. Skin‚ absorbs. Stomach and intestinal lining‚ filters. The kidney‚ secretes. Forming of glands‚ closely attached to each other forming a protective barrier‚ always has one free surface open to outside the body or inside an internal organ‚ has no blood vessels but can soak up nutrients from blood vessels in connective
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Unit 3 Lecture Study Guide 1. List the 4 primary tissue types and give the general characteristics and functions of each one. a. Epithelium - 1) It protects us from the outside world – skin. 2) Absorbs – stomach and intestinal lining (gut). 3) Filters – the kidney. 4) Secretes – forms glands. Characteristics (Traits): 1) Closely attached to each other forming a protective barrier. 2) Always has one free (apical) surface open to outside the body or inside (cavity) an
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Anthropology Science of human cultural and biological variation and evolution Study of human biological and cultural difference across space and time Anthropos: man (Greek) Logos: word (Greek) Naming: building a knowledge off “Study of Man” Human Diversity/Differences Rigorous explanation of being human appreciation of many things Overlap with other fields Economics: accumulate wealth v. giving away wealth Different perspectives Biological and Cultural Differences Important
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Susan Griffin compares and contrasts cellular life and weaponry as she writes her essay‚ Our Secret. She uses these ideas together with characters and events‚ to help explain causes and effects in the essay. She alternates from the cell’s function to the history of weaponry throughout the essay. With both these ideas‚ she starts at the elementary level‚ with a cell’s life and with the Vergeltungswaffe missile‚ and tells how they progress. In this essay‚ I will describe how Griffin uses cellular life
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Biology‚ 7e (Campbell) Chapter 17: From Gene to Protein Chapter Questions 1) Garrod hypothesized that "inborn errors of metabolism" such as alkaptonuria occur because A) genes dictate the production of specific enzymes‚ and affected individuals have genetic defects that cause them to lack certain enzymes. B) enzymes are made of DNA‚ and affected individuals lack DNA polymerase. C) many metabolic enzymes use DNA as a cofactor‚ and affected individuals have mutations that prevent their enzymes
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