Proteomics is the study of proteomes‚ which are groups of proteins in living systems. It is used to examine when and where proteins are being expressed‚ rate of degradation‚ modifications‚ how they interact with other proteins and more. The goal in this lab was to determine the similarities and differences in muscle proteins among 5 species of fish using SDS-PAGE and Western blotting. First‚ proteins are separated based on size. Second‚ antibodies are used to detect the protein of interest. Lastly
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Biofuel Enzyme Kit Katie Adamson Biochemistry Laboratory‚ BIO124L 1/29/15 Abstract The objective in this lab was to determine the effects different conditions had on the enzyme cellobiase. We examined reaction rates in the presence or absence of an enzyme‚ the effects temperature and pH changes on the enzyme and the effects enzyme concentration and substrate concentration had on the enzyme. As expected results showed us that cellobiase works optimally when conditions are favorable. We see this
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reportChemistry 117L Laboratory Report Name: Aneesa Noorani Lab Day: Tuesday Lab Room: SCL 114 Date of Experiment: January 22‚ 2013 TA: Mikhail 1. Basic Laboratory Skills Purpose(s) of the Experiment: The purpose of the first part of today’s experiment is to establish the stoichiometry of the reaction between titrate oxalate (C2O42-) and permanganate (MnO4-). The purpose of the second part of today’s experiment is to learn about the concepts of the rate of chemical reactions and homogenous
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Introduction The purpose of this lab was to calculate and determine the optimal dosage of ultraviolet (UV) light to expose Saccharomyces cerevisiae (S. cerevisiae) to that would result in a survival rate of 20-40%. Saccharomyces cerevisiae commonly referred to as baker’s yeast is a single-celled eukaryote. Yeast is capable of growing as either a haploid or a diploid cell. The diploid cell can reproduce mitotically or sporulate‚ a process initiating meiosis and forming an ascus with four spores (Sacchromyces
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Introduction and Purpose: This lab focuses on how weight affects the time it takes for a rotocopter to fall from a drop height of 1 and 2 meter(s) respectively. The primary goal of this lab is to investigate and explain the relationship of these two factors. During the process of conducting this experiment‚ learning to design a lab will also be achieved. Research Question: What is the effect of weight on the time it takes for a rotocopter to fall from a drop height of 1 and 2 meter(s)? Variable
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NAME: Donna Ha TITLE: Measuring the Rate of Osmosis Using “De-shelled” Chicken Eggs INTRODUCTION: In order to fully understand the concept of this experiment‚ one must first be introduced to diffusion. Diffusion is a spontaneous process by which molecule particles move from one area that is highly concentrated to another area in which its concentration is lower. Cells contain fluids and are surrounded by fluids; in order for a cell to function it is required to be in a balanced state. The
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(100 ºC) and an acidic pH. Enzyme C produced the most maltose at 37 ºC (body temperature) and a pH of 7 (neutral). Conclusion In a prior lab‚ it was given that the enzyme Aspergillus oryzae (fungus) works best at warm temperatures and an acidic pH‚ porcine (pig) works best at body temperature (37 ºC) and
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Experiment-5 Thermal physics is the combined study of thermodynamics‚ statistical mechanics‚ and kinetic theory. This practical is done to measure the temperature drop of water over a period of time (30 minutes). Research question- What is the rate of temperature change over a period of 30 minutes? Independent variable- time Dependent variable- the temperature of water Controlled variables- the amount of water in the calorimeter - The stop watch
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FeCl3‚ changes in color will occur. Pure Aspirin has a melting point around 135°C and a pure salicylic acid has 158 °C. TLC is used to separate compounds based on affinity for stationary phase or mobile phase. In TLC‚ the solvent went up at different rates. The retention factor (Rf) of a component can then be measured by dividing the distance that was traveled by the solvent front distance.By doing this experiment‚ the number of moles and grams of salicylic acid and acetic anhydride can be answered.
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Lab Report Procedure 6.1: Perform Benedict’s test for reducing sugars. Introduction: Benedict’s tests allows for the detection of the presence of reducing sugars. All monosaccharides are reducing sugars since all of them have active carbonyl group. Some disaccharides that are exposed to a carbonyl group are also reducing sugars but less reactive than monosaccharides. By mixing the sugar solution with Benedict’s solution and heating them‚ a redox reaction will occur. The copper (II) sulphate
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