UNKNOW BACTERIA LAB REPORT UNKNOWN 36 Introduction The purpose of this lab was to identify two unknown bacteria from a mixed culture. The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. This is important in the medical field because identification of unknown bacteria can help treat a patient by knowing the contributing source of a disease. Also knowledge of different bacteria helped others
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Methicillin-resistant staphylococcus aureus (MRSA)‚ is a gram positive bacterial that is normally found on the skin and in the throat‚ and is a life threatening staphylococcal infection that may arise within hospitals‚ and “is frequently responsible for abscesses‚ endocarditis‚ impetigo‚ osteomyelitis‚ pneumonia‚ and septicemia.” Treatment usually includes bed rest‚ analgesics‚ and an anti-microbial drug that is resistant to penicillinase‚ an enzyme secreted by many species of staphylococcus. This writer
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My journey to the unknown started about three weeks before the end of my microbiology class at Brunswick Community College. This journey started out with a media plate cultured with an unknown species of bacteria. I was then instructed by my professor to use all of my previous knowledge to identify the unknown bacterial pathogen. This lab led me to master my skills of using the Gram stain‚ testing for catalase‚ testing for coagulase and using the oxacillin screen. Now I am going to take you with
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unstained microorganisms‚ the wet mount is easier to perform and usually provides sufficient information. Objective To become familiar with method to examine the living bacteria. Materials 1. 24 hours nutrient broth cultures of a) Staphylococcus aureus b) E. coli 2. Hollow ground depression slides 3. Inoculating loops
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References: “Methicillin-Resistant Staphylococcus Aureus (MRSA)-Overview.” WebMD. WebMD‚ n.d. Web. 29 Apr. 2013. McDonald‚ Virginia‚ Mary Thoele‚ Bill Salsgiver‚ and Susie Gero. Lab Manual for General Microbiology. N.p.: n.d. Print. 101010 0411.
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diarrhea. Staphylococcus is a genus of the Gram-positive bacteria which appears as grape-like clusters structure when observed under the microscope. Staphylococcus genus can be grouped into not less than 40 species‚ where nine of them consist of 2 subspecies and one gas three subspecies. Staphylococcus was first found in the year if 1880 by surgeon A.Ogston during a surgical abscess in a knee joint. Until this day‚ average estimation of 20% of the human population are carriers of Staphylococcus aureus
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test was then used to confirm these results‚ since it has the ability to select for gram negative bacteria (Figure 9). With negative results for this test‚ a variety of bacteria were able to be eliminated from the list of bacteria‚ including Staphylococcus aureus‚ Pseudomonas aeruginosa‚ and Enterobacter aerogenes. The EMB test (Figure 10)‚ helped to further eliminate more bacteria including E. Coli. This confirmed our results along with the negative results for the Indole test and the Sulfur reduction
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animals. Washing hands is a simple‚ inexpensive‚ and an effective action to prevent the spread of microbes that cause healthcare associated infection. The most common transmissible disease in any hospital‚ dental‚ or veterinary cleaning is Staphylococcus aureus. The task of proper hygiene in-between patients’ care enhances patient safety
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that had been "controlled" through the use of antibiotics are beginning to develop drug resistance and therefore re-emerge as serious threats in the industrialized world as well as developing nations. (World Health Organization‚ April 2014). Staphylococcus aureus strains are emerging that are resistant to many of the antibiotics that were previously effective against them. These staph infections are of great concern in hospital settings around the world. Patients with infections caused by drug-resistant
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within a 5 minute range. The bacterial suspension used for paired at different concentration data was Staphylococcus Aureus for which gave good results when the reduction factor value of 1.20x106 ml-1 was obtained for the organism as it was above the value 105 which was used a guideline for effectiveness of the test. For class data there was four organisms investigated which were: Staphylococcus Aureus‚ Enterococcus hirae‚ Pseudomonas aeruginosa and Escherichia coli. In general the disinfectant didn’t
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