it is called bateriostatic. (Fundaments of Microbiology Lab Manual) The purpose of this experiment was to examine the effect different disinfectants and antiseptics‚ which are used to decrease the number of microbes‚ on Escherichia coli and Staphylococcus aureus. The effectiveness of the chemical agent was determined by the size of the zone of inhibition around the disc incubated on soft nutrient agar seeded with the two bacteria on separate plates. For E. coli‚ a gram negative organism‚ the zone
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traditional practice hence‚ this study. The disc diffusion method was used to determine the antibacterial activity against gram positive and gram negative bacteria (standard strains and clinical isolates). Antibacterial activity was tested on E. coli‚ S. aureus‚ B. subtilis and P. aeruginosa. Results show that there was no formation of any zone of inhibition as observed after 24 hrs. Thus‚ this justifies the traditional use of the plant as decoction treatment for various ailments. Various concentrations
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sensitive‚ intermediate‚ or resistant; and report to TA. Procedure: Selective and Differential Media 1. Label the MacConkey agar plates. Streak the plates for the following organisms: Enterobacter aerogenes‚ Salmonella enterica‚ and Staphylococcus aureus‚ and sterilize the loop after streaking each quadrant. 2. Label a sheep blood agar plate and use a loopful of Enterococcus faecalis to inoculate it. Streak the plate. 3. Repeat step 2 for Streptococcus pyogenes 4. Transfer an A disc
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EXAM ONE Cheating Question – I acknowledge I won’t cheat. You expose the bacteria Staphylococcus aureus to low levels of antibiotic methicillin. The surviving bacteria are then exposed to higher concentrations of methicillin and the process repeated until a strain of methicillin-resistant S. aureus (MRSA) was generated. To test your hypothesis‚ you perform an experiment by spreading the original strain of S. aureus and the MRSA strain onto agar plates containing doses of methicillin used in the hospital
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Methanolic Extract of Eggplant (Solanum melogena) Peel as a Dye Pigment in Differential Stains Azucena‚ Alexis G. An Official Entry to 2010 Regional Science Fair Cluster 2 Physical Science- Individual Negros Occidental National Science High School January 13‚ 2011 Ma. Melanie P. Romero Research IIB Adviser APPROVAL SHEET The research paper attached hereto‚ entitled‚ “Methanolic Extract of Eggplant (Solanum melogena) Peel as a Dye Pigment in Differential Stains”‚ prepared and submitted
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activity of silver in general‚ and of silver nanoparticles in particular‚ is of significant interest because it appears to be independent of the strain of bacteria. Crucially‚ antibiotic resistant strains‚ MRSA (methicillin‚ or multiple-resistant Staphylococcus aureus)‚ E. coli O157‚ and others are affected by silver [6]. The reasons for this are not fully clear as yet‚ but could be related to mechanisms of silver ion action on bacteria [110]‚ trypanosomes and yeasts‚ all of which can take up and concentrate
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http://www.highlands.edu/academics/divisions/scipe/biology/labs/rome/bacterial_smears_and_stains.htm * Microbiology Techniques http://www2.hendrix.edu/biology/CellWeb/Techniques/microstain.html * Simple Staining http://www.microbiologyinpictures.com/staphylococcus%20aureus.html
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A Brief History of Antibiotics‚ Antibiotic Resistance‚ and Antibiotic Alternatives Antibiotic Resistance and Alternatives Antibiotics have been commonly‚ though mistakenly‚ thought of as the ultimate cure‚ for almost all illness‚ for over half a century now. However‚ the intended use of antibiotics is for the treatment of bacterial infections and diseases. Viruses or fungi-related illnesses will not be affected by antibiotics. This misunderstanding of the use of antibiotics has led to overuse
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Communicable Disease Paper Kayla Lysak HCS 457 July 7‚ 2014 Lindsay Cogan Communicable Disease Paper This paper will be discussing what MRSA is and the efforts that are being made to control it. It will also be discussing the environmental factors that are related to this disease. Other factors that will be discussed will be to explain the influence that lifestyles‚ socioeconomic status and disease management play and what the public health department is doing to reduce the threat this disease poses
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different bacterial species have been isolated from fish-processing environments‚ e.g. Exiguobacterium spp. has been isolated from a fish processing plant in Japan (Yumoto et al.‚ 2004) and Staphylococcus spp. in food is often associated with human contact. Furthermore‚ due to the halophilic nature of Staphylococcus spp. (Simon
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