in killing Staphylococcus aureus (b) obtain the statistical difference of effectiveness in killing S. aureus between hand soap and alcohol gel. The null hypothesis is that neither hand soap nor alcohol gel is effective in killing S. aureus more than the control group which is nothing at all. The alternate hypothesis is that hand soap versus the control group will be more effective in killing S. aureus and/or alcohol gel versus the control group will be more effective in killing S. aureus. Another alternate
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Biology 1003 - Cells: Size‚ Structure and Microscopy Introduction What is life? What are the key factors that biologists have defined that apply to every single organism on the planet? In the 17th century‚ due to the amazing advancement of the microscope‚ biologists created a theory to differentiate the living from the non-living called the cell theory. One concept from the theory is that all living organisms are composed of one or more cells. The importance of studying cells is akin to the
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Pure Culture Techniques In this first lab‚ you will be learning some very fundamental and important techniques. As is the case with most things‚ shorts cuts usually get you in trouble. This is especially true in Microbiology. The techniques you will be learning tonight‚ if mastered correctly‚ will make your life and learning experience in Microbiology much easier‚ if you don’t pay attention and practice these techniques incorrectly‚ well then……? Today you will be learning the following techniques:
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consumed and the ingested microorganism produces toxin itself causes the illness. Some examples of bacteria that cause food are CLOSTRIDIUM BOTOLINUM and STAPHYLOCOCCUS AUREUS • An intoxication may also occur due to consumption of a toxic chemical such as cleaning chemicals. Foodborne Intoxication • Clostridium Botolinum Staphylococcus Aureus 3. Toxin Mediated Infection • Ingestion of a harmful microorganism in food that produces a toxin in the
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185-195 2. Cébrian G‚ Condón S‚ Mañas P: Heat-adaptation induced thermotolerance in Staphylococcus aureus: Influence of the alternative factor sigmaB. Interational Journal of Food Microbiology 2009‚ 135(3): 274-280 3. Gasper B: Laboratory Period 6 – Effect of Osmotic Stress and Temperature on Microbial Growth. BIO 3400 Microbiology Laboratory Manual 2012‚ 1: 43-47 4. Graham JE‚ Wilkinson BJ: Staphylococcus aureus osmoregulation: roles for choline‚ glycine betaine‚ proline‚ and taurine. Journal of
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concentrations used for all the extracts was 500 mg/ml. Methanolic extract of C. album was found to be most active among all other extracts and inhibits Staphylococcus epidermidis‚ Bacillus subtilis‚ Staphylococcus aureus‚ Escherichia coli. The extract of a mixture of solvents was also found to be effective in inhibiting Staphylococcus aureus. Methanolic extract of C. album was found to give a most clear zone of inhibition. The antibacterial activity is attributed to the phenolic content present
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least in a laboratory setting‚ says Mwambete‚ who tested it for action against several types of infection. Mwambete found that the plant may be useful for combating Escherichia coli‚ the common yeast Candida albicans‚ Salmonella typhi and Staphylococcus aureus. It may be useful against Klebsiella pneumoniae as well‚ Mwambete notes. Klebsiella pneumoniae is frequently a hospital-acquired pathogen. It causes pneumonia‚ urinary tract infections and intraabdominal infections‚ according to the Centers
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populations in health care facilities. Other problems associated with regulatory pressures are also demonstrated by the fact that Medicare is planning to no longer reimburse for hospital-acquired infections– particularly Methicillin Resistant Staphylococcus aureus (MRSA). The Center for Disease Control suggests that there are two main ways to contract any of the common viral illnesses. Contracting may occur through coughing or sneezing with airborne illness (known as “droplet spread”)‚ or through
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that some of these membrane-active compounds can enhance the activity of conventional antibiotics‚ eliminate natural resistance to specific antibiotics (reversal of resistance) and exhibit strong activity against multidrug-resistant forms of Staphylococcus aureus‚ Escherichia coli‚ Salmonella spp.‚ Mycobacterium tuberculosis‚
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IDENTIFYING GRAM POSITIVE COCCI As mentioned in Exercise 8‚ “Identifying Gram Negative Rods”‚ identifying bacteria is a common activity in the microbiology lab. Like the game Clue™‚ each time you gather a piece of information to solve the mystery‚ you gather some information that supports some identities and eliminates others from contention. In the lab‚ the process continues as you gather more information until only one microbe remains and all others have been eliminated as possibilities. Thus
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