Methyl Red and Voges-Proskauer Test The Methyl Red test shows which bacteria are creating stable acids through mixed acid fermentation of glucose. This helps to identify enteric bacteria by examining how they metabolise glucose. Every enteric bacteria first produces pyruvic acid from metabolism of glucose. A methyl red positive enteric bacteria‚ uses the mixed acid pathway when breaking down pyruvic acid to different acids‚ like lactic‚ acetic‚ and formic acids. The Voges-Proskauer test determines
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DETERMINATION OF THE ACID DISSOCIATION CONSTANT OF METHYL RED M.C. Caligagan ‚M.N.Q. Tolentino and M.Q. Clores Institute of Chemistry‚ College of Science University of the Philippines‚ Diliman‚ Quezon City‚ Philippines Received :September 21‚2011 ABSTRACT This experiment aims to determine the acid dissociation constant value of Methyl Red by means of spectrophotometry. Ten samples were spectrophotometrically analyzed under a UV-Vis spectrophotometer and from the absorbance values acquired‚ calibration
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Simple Distillation of Ethyl Acetate contaminated with Methyl Violet Operational OrganicChemistry A Problem Solving Approach to the Laboratory Course‚ 4th edition‚ by John Lehman‚ Experiment 4‚ OP-30 pg 710-719 I. Introduction The students will use the distillation method to extract pure Ethyl acetate by using the distillation equipment. The distillation is the process of vaporizing a liquid mixture in one vessel and condensing the vapors into the receiver. II. Experimental procedures. 1
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present in a sample. Primarily‚ the aim of Experiment 1 was to measure the absorption spectrum of a particular coloured substance (in this case Bromophenol Blue and Methyl Orange) at varying wavelengths of light. The goal of this experiment was to measure the given amounts of a given chemical substance that was present in a given sample of Methyl Orange and in blood. We measured the optical density at wavelengths 400 to 700 in intervals of 20 mu. For Experiment 2‚ the process of the experiment focuses
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manufacturer to determine if sodium benzoate‚ a common food preservative‚ undergoes significant chemical changes at the pH level encountered in the acidic environment of the stomach. Experimental Method Sodium benzoate was prepared by addition of an equimolar amount of aqueous sodium hydroxide (3 M‚ ~ 20 ml) to a benzoic acid (1.256 g‚ 10.3 mmol) /water mixture (10 ml). Benzoic acid is insoluble in water. The hydroxide base rapidly deprotonates the acid‚ producing the benzoate anion which is readily soluble
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of Salicylic Acid from Wintergreen Oil I. Introduction and Purpose Until 1874 commercial salicylic acid was synthesized entirely from natural wintergreen oil. In this experiment the salicylic acid is reproduced using methyl salicylate‚ the major constituent of wintergreen oil and later compared to salicylic acid made from benzene. The purpose of this lab is to determine the difference between salicylic acid made from methyl salicylate and salicylic acid made from benzene comparing the melting
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extraction‚ crystallization and melting point determination‚ preparations of organic compound and separation of mixtures. The Techniques Students are REQUIRED to read and understand the various techniques that will be covered in the laboratory sessions from the textbook. | Techniques | Page | 1 | Laboratory Safety | 6 | 2 | Laboratory Glassware | 31 | 3 | Measurement of Volume and Weight | 39 | 4 | Heating and Cooling Methods | 58 | 5 | Reaction methods (eg: distillation‚ reflux) | 68 |
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lab’s purpose was to determine whether Salicylic Acid made from methyl salicylate is the same as Salicylic Acid made from benzene. This information can be found by comparing the difference in melting points of the two samples of Salicylic Acid. Here are the physical properties of Salicylic Acid and the mechanism of reactions that occurred in this lab. How Salicylic Acid is derived: Procedure/Results First‚ .26mL of 2.0mmol methyl Salicylate was measured and put into a reaction vial along
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Salicylic Acid from Wintergreen Oil Objective – Preparation of salicylic acid (organic synthesis) from methyl salicylate utilizing previously used procedure from the nineteenth century. The final product will then be evaluated in comparison to salicylic acid made from benzene. Discussion – In this synthesis‚ methyl salicylate is the starting material or precursor and salicylic acid is the target product. It is the major constituent of wintergreen oil. The difference in structures between methyl salicylate
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Assignment of internship Topic: Drug listing of pharmacy of Quaid-e-Azam international Hospital Submitted to: Dr.Nowsherwan Kiyani and Dr.Asif Submitted By: Sundas Javed Dated: 24july‚2013 Index 1. Vials 2. Ampoules 3. Syrup‚ suspension and elixers 4. Ear drop‚ eye drop and ointment 5. Nutritional Powder 6. Cream paste and ointment 7. Tablet and capsule 8. Fridge item 9. Infusion Vials Brand Name Generic Name Dosage Form Strength Classification Indications
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