Discussion: The two dyes identified were disperse blue 3 and disperse red 9. Disperse red 9 separated first because it is less polar than disperse blue 3. This is due to the structure of disperse red 9 being more symmetrical than that of disperse blue 3 and having more nonpolar bonds. Disperse blue 3 is more polar because it has a hydroxide bond and has a larger dipole. The principle behind using column chromatography is that it separates compounds based on polarity. The alumina serves to allow
Premium Chromatography Thin layer chromatography Separation process
significant difference in Daphnia’s response when exposed to red light compared to white light. Thus‚ the null hypothesis was rejected and the experimental hypothesis was accepted. These findings could be due to the negative phototactic response that occurs when they are exposed to visible light that are above 600 Mn (nanometers) that are also considered the color red. This shows that Daphnia have a greater phototactic reaction to red light than when exposed to white light. When compared to a similar
Premium Light Electromagnetic radiation Experiment
specially. This funnel is called as V-funnel. This test is a self-compactibility test to measure the capacity of concrete to pass into the spaces. This test determines the viscosity and passing capacity of SCC. Fresh SCC is filled to the funnel without any compaction or vibration and then the sliding cover under the funnel is opened and the filling time of all concrete in the funnel to the pail is recorded. Fig. 2 V-funnel test L-box test L-box test is used to assess the passing ability of SCC to
Premium Automobile Internal combustion engine Petroleum
Unknown Lab Report April 25th‚ 2006 Introduction The purpose of this lab was to identify two unknown bacteria cultures using various differential tests. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways‚ each was used in a way to help recognize those specifics and identify the unknown cultures
Premium Enzyme Staphylococcus Staphylococcus aureus
MEMORIAL UNIVERSITY OF NEWFOUNDLAND FACULTY OF ENGINEERING AND APPLIED SCIENCE Engineering: 4312 Mechanics of Solids I Lab Test #4 – Torsion Test OBJECTIVES: To carry out a torsion test to destruction in order to determine for a 1020 carbon steel rod specimen: 1. The modulus of rigidity‚ 2. The shear stress at the limit of proportionality‚ 3. The general characteristics of the torque‚ angle of twist relationship. REFERENCES: 1. Hibbeler‚ R. C. "Mechanics of Materials"‚ Prentice-Hall
Premium Force Shear strength Shear stress
3.7.2.1.5 Urea test PRINCIPLE Urea is hydrolyzed in the presence of urease to produce ammonia and CO2. The ammonia produced combines with 2 – oxoglutarate and NADH in the presence of GLDH to yield glutamate and NAD. Urea + H2O + 2H+ 2NH4+ + CO2 NH4+ + 2-Oxoglutarate +NADH H2O +NAD+ + Glutamate The decrease in absorbance due to the decrease of NADH concentration in unit time is proportional to the urea concentration. Ammonia
Premium Sodium hydroxide Sodium Sodium chloride
Zebrafish Embryotoxicity Test (ZET) The ZET will be done following the method proposed by Beekhuijzen et al. (2015) with modifications. Six embryos will be exposed to each of the following treatments: 0.1‚ 0.05‚ 0.025‚ 0.0125‚ and 0.00625 mg/ml. Embryos will be incubated in 24-well plates with built-in lids. Each well will contain one embryo and 2 ml of the respective treatment. All test solutions will be renewed every 24h to maintain the integrity of the test concentrations. The embryos will
Premium DNA Gene Bacteria
As a result of the tests ran‚ I was able to identify the unknown bacteria. For Unknown A‚ I ran six tests. I first isolated the bacterium from the second bacterium and found a clear growth (Table 1‚ Figure 1). Secondly‚ I ran a gram stain and found a gram positive‚ cocci bacterium (Table 1‚ Figure 2). Third‚ I ran a catalase test in which was negative (Table 1). From here‚ I determined a starch hydrolysis test would be necessary to distinguish between different bacteria. The result was negative (Table
Premium Bacteria Microbiology Staining
Cold pressor test is widely used as test for auto- nomic functions. It has been used as diagnostic test for diseases like hypertension and other cardiac auto- nomic disorders. During cold pressor test ‚ the hand or foot is immersed in cold water bath held at a con- stant temperature at 80 C. Cold water immersion activates afferent pain and temperature neurons‚ re- sulting in a centrally mediated stimulation of sympa- thetic efferent neurons. In healthy subjects ‚ the CPT causes increase
Premium Autonomic nervous system Autonomic nervous system Sympathetic nervous system
the central analgesic activity of AVS022 and its components on hot-plate test Initial studies utilizing the hot-plate test
Premium Rat Rat Inflammation