performed this test‚ the initially slant for unknown microorganism #17 is important. For this lab‚ two identical slants are used for two reasons. Firstly‚ the slant can be used to make sure that there is no contamination from the Nutrient Agar plate. Secondly‚ the second slant will become a stock culture to prevent the shortage of slants during performing the series of tests. Kliger’s Iron Agar tests can be used to determine multiple characteristics of unknown microorganism #17. Kliger’s Iron Agar slants
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The unknown soda ash from experiment 3 was used‚ to determine the weight for each trial we used the equation of (M of HCl) x (18 ml x 105.99) / (10 x 2 x Na2CO3 ). Which was equal to (0.01472 M) x ((18 mL X 105.99)(10 x 2 X 2.428 % )= 0.6 g. To start we had to rinse the beakers‚ electrode and the stirring bar with diluted water. The sample we needed was weighted to the closest 0.1 mg which we got was 0.3 for the first trial. The sample was transferred to a 250 mL beaker and dissolved in 70 mL of
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material Aerial parts of wild-growing M. officinalis were collected during April from Aum-Romanna (Jordan) by one of us (EYQ). The plant material was identified and authenticated taxonomically at the Hashemite University herbarium. A voucher specimen was deposited under the number HU-437 at the Hashemite University herbarium‚ Zarka‚ Jordan‚ for future reference. Determination of essential oil composition Samples of dried aerial parts (300g each sample) of M. officinalis were hydrodistilled for 4
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Pathogenic Micro MLST assignment 1. MLST uses the sequence information within a set of housekeeping genes to determine the type of the organism. For each gene the dissimilar sequences are noted to be different alleles. MLSA is very similar to MLST but uses linked sequences to derive a phylogenetic relationship. MLSA is generally used to progress species descriptions whereas MLST is used with species that are already distinct. In this lab we are performing MLST. 2. The 16s rRNA gene is highly conserved
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which organism we had in our unknown mixed culture tube by running a series of experiments to detect which specific Gram negative organism we had. To detect your gram positive from the mixed culture was given as extra credit points also. A Gram stain was performed and isolation streak plate in order to isolate and observe the unknown organism. Before the series of test‚ a dichotomous key had to be written up in order to know what steps and tests to run to identify the unknown Gram negative organism. I
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The micro-hardness test can measure the case to core depth parts. Micro-hardness testing is required where the superficial hardness test is not conceivable due to material’s small geometrical shape. The welded part we were going to examine‚ experienced the similar criteria since the welded bead of the joint was way too small. To test the hardness of our welded area we had gone through a micro-hardness test using a Shimadzu micro-vicker hardness testing machine. In
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(CM) | Radius (CM) | M&M Thickness (CM) | 1 | 75 | 11.34 | 5.67 | 0.722 | 2 | 83 | 12.68 | 6.34 | 0.658 | Table 2 - Direct Measurement | Trial | M&M Thickness (CM) | 1 | 0.642 | 2 | 0.741 | 3 | 0.683 | Table 3 - Calculated Averages | Method | Calculated Average Thickness (cm) | Indirect (from Table 1) | 0.700 | Direct (from Table 2) | 0.689 | Questions: 1. When you performed Step 2 of the procedure‚ you actually made a cylinder of M&Ms. The cylinder was
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Introduction The acid-fast stain was developed in 1882 by Paul Ehrlich. Ehrlich was working with Mycobacterium tuberculosis‚ the bacilli responsible for tuberculosis‚ and found a technique that renders M. tuberculosis distinguishable from nearly all other bacteria. Acid-fast staining is‚ therefore‚ known as a differential stain. Mycobacterium and some Nocardia species are considered acid fast because‚ during the acid-fast procedure‚ they are able to retain the primary dye even when decolorized
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It Smells Like Proteus vulgaris Microbiology Lab Report for Unknown Robert Bhowanidin MCB 2010L / Section 1290 October 24th‚ 2013 The following report will describe both my journey to find my unknown as well as the results that led to my discovery. Before I start‚ I will say that I am 100% positive that my unknown (which was #31) is none other than Proteus vulgaris. My data and the ensuing results from them simply cannot be disputed. From my first batch of results‚ Proteus vulgaris reared
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Gram Positive Unknown: “Enterococcus faecalis” Family: enterococcaceae Genus: enterococcus Species: faecalis Gram + Oxygen class: facultative anaerobe Temperature class: mesophile – they can grow in the range of 10 °C - 45 °C pH class: can grow at a pH range of (4.6 – 9.9) with the optimum at 7.5 Enterococci can survive very harsh environments including extreme alkaline pH 9.6 and salt concentrations (basic). Environment: They can
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