Wilmott AIM: To calculate the quantity of observable microbial colonies on the surface of the Agar solid‚ as to determine the presence of microbes in consumable products i.e. yoghurt and blue vein cheese. HYPOTHESIS: Microbial growth will be present in two of the three Agar plates (those containing the food product) due to the suspected presence of microbes‚ whilst the control Agar plate (containing no food products) will remain free of contamination and microbial growth. MATERIALS: - 3x Agar
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specimens in test tubes A‚ B and C are allowed to incubate for 2 hours. 8. Mark the 3 petri dishes - A‚ B and C. Remove the lid and using the syringe‚ extract 10 ml of the sample mixture from test tube A and place it in the center of petri dish A. 9. Use a new syringe to extract a 10 ml sample from test tube B and place it in dish B and repeat for test tube C/dish C. 10. Replace the petri dish lids and store the petri dishes in a cool and shaded
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and analyzing the various microbes regularly encountered in the daily human environment becomes quite apparent when one gains even a very basic knowledge of how diseases are acquired and spread. The purpose of this lab was to collect and observe microbes from environmental and human
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antimicrobial chemicals‚ radiation‚ etc. The viable count is most common or standard method used to quantitate bacteria. With this method only microbes that are alive and able to reproduce can be counted. Since microbes grow into such large numbers it is extremely difficult to perform this without diluting the microbial culture first. A key concept here is the ability of microbes such as bacteria and fungi to form visible colonies when grown on solid media. This can also be used to count viruses but instead they
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Instructor: Dirk VandePol Date: 6/21/2013 Streak Plate Isolation for Obtaining Pure Culture 1. When an agar plate is inoculated‚ why is the loop sterilized after the initial inoculation in put on? Ans: We use agar plate to inoculate microbes by zipping the loop on the agar several times. We streak on the agar plate four time‚ propose is to isolate the unknown bacteria. Therefore‚ the first time to streak on the plate‚ there are million of bacteria on the loop. For that reason‚ we need
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agents in comparison to antibiotics. The main differences between antiseptics and disinfectants are that disinfectants are used primarily to sanitize objects‚ and antiseptics are used on human skin. They both act as antimicrobials that kill the microbes that cause infection‚ and contain many of the same ingredients. Disinfectant usually comes in liquid form or in a spray‚ whereas antiseptic is usually in the form of an ointment. Objectives To be able the students to differentiate and observe
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reproductive spore. Reproductive Spore = a means of both reproduction and dissemination of molds‚ since they are readily carried about by air currents. Septa = hyphal cross walls which divide the filaments into separate cells. Petri Plate (dish) = a special covered dish in which mold is cultured. Medium = a solid nutrient used for culturing. Agar = a non-nutrient thickening agent which is thicker than gelatin but still quite soft. Smear = a thin film of microbial cells on a microscope slide
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was determined what antiseptic or disinfectant was able to best inhibit this kind of bacterial growth. Nutrient Agar was poured into a Petri dish with four quadrants and then a pipette was used to place bacterial culture on top. Using forceps‚ a filter disc was dipped into each inhibitor and then into a separate quadrant of the Petri dish. The lid of the Petri dish was taped on using masking tape and then
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type of plants. Introduction A substance that kills or prevents the growth of microorganisms for example bacteria‚ fungi or protozoans is called an antimicrobial. This substance has 2 major roles which are to either kill microbes (microbiocidal) or prevent the growth of microbes (microbiostatic). Disinfectants are antimicrobial substances used on non-living objects outside the body. This substance included antibiotics‚ antifungals‚ antiprotozoals and antivirals.(1) For a long period of time‚ plants
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|should be pre-sterilised using heat and pressure. All the vessel openings should have filters to prevent microbes| | | |from entering. Suitable pH‚ temperature and oxygen levels must be provided for the growth of the microbe of | | | |interest. Aeration can be forced using a sparger‚ which may be used to improve microbe-nutrient mixing. A | | | |stirring paddle may also be used. The addition of acid/alkali
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