Laboratory Exercises in Microbiology‚ Fifth Edition Front Matter Preface © The McGraw−Hill Companies‚ 2002 PREFACE Take interest‚ I implore you‚ in those sacred dwellings which one designates by the expressive term: laboratories. Demand that they be multiplied‚ that they be adorned. These are the temples of the future—temples of well-being and of happiness. There it is that humanity grows greater‚ stronger‚ better. Louis Pasteur (French chemist‚ founder of microbiology‚ 1822–1895) There
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oasis of life that resides around the earth ’s nourishing thermal vents. Here‚ a vast community of organisms thrive under very harsh circumstances‚ and most importantly‚ in the absence of life-giving light. How is this possible? Chemoautotrophic microbes. Chemoautotrophic bacteria are the primary producers on the ocean floor‚ where light cannot reach. Unlike most organisms‚ these bacteria do not need carbohydrates‚ vitamins‚ protein‚ and sugar to create energy‚ and ultimately to survive. Instead
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Microbiology: ‘The Correct handling of Micro-organisms’ 1. Devise a title for each of the two experiments you did : (i)‚ Experiment 1 demonstrated the growth of bacteria when placed in liquid nutrient broth culture‚ the number of species present had increased in growth. .(1) (ii) Experiment 2 illustrated the growth of bacteria when placed on different surfaces of solid agar plates which included: nutrient agar‚ CLED agar and MacConkey agar; the number of species present also had increased in growth
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Observe the dish and count the number and types of colonies. Record the results in Data Table 1: Environmental Colony Formation‚ in the Lab Report Assistant section. 7. Soak the dish in a 10%-bleach solution for 1 hour and then discard it. Part iii: Microbes in the Soil 1. Choose an environmental site to collect a soil sample. Then use a new sample cup to collect a soil sample. 2. Pour distilled water into the cup‚ so the water sits just above the soil level. Mix the water and soil well. 3. Let the
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Microorganisms are classified according to their structure. By means of flow charts‚ diagrams and tables explain the differences between Viruses‚ Bacteria‚ Cyanobacteria‚ Achaea and Fungi. Bacteria or bacterium are unicellular microorganisms. They are essentially only a few micrometres long and form of various shapes including the spheres‚ rods and spirals. A BACTERIAL CELL Illustration courtesy of Wikipedia. A Virus (from the Latin noun virus‚ meaning toxic or poison) is a sub-microscopic
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1 Student: ___________________________________________________________________________ 1. Extant microorganisms are organisms from the fossil record that are no longer present on Earth today. True False 2. All cellular organisms can be placed into one of three __________‚ which include the Bacteria‚ Archaea‚ and the Eukarya. ________________________________________ 3. Archaea are cellular organisms that have unique cell membrane __________. ________________________________________
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{draw:frame} Aseptic technique was developed by Joseph Lister in 1867. He used diluted phenol to cleanse surgical wounds and equipment. He also used carbolic acid aerosol to prevent harmful microorganisms from entering the surgical field or contaminating the patient. Aseptic technique includes hand washing‚ the use of sterile gloves‚ mask‚ and gowns‚ sterilization of surgical instruments and other equipment‚ and the use of disinfectants‚ including antiseptics. The definition of asepsis is: 1)
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How to Carry Out Aseptic Techniques in a Batch Culture and in the Laboratory | | | | | | | | | |The batch vessel should be sterilised beforehand using steam. The nutrient medium that is added to the vessel |
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disease that persists over a long duration. Control - control group covalent bond - A chemical bond formed by the sharing of electrons between two atoms. disinfect - The destruction of pathogenic nonsporulating microbes or their toxins‚ usually on inanimate surfaces. Electrolyte - Any compound that ionizes in solution and conducts current in an electrical field. Electron - A negatively charged
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Microbiology is the study of living organisms that are too small to be clearly seen by the unaided eye. The science of Microbiology dates back only to 200 years‚ yet the recent discovery of Mycobacterium tuberculosis DNA in 3000 year old Egyptian mummy reminds us that microbes have been around for much longer. Infact‚ Bacterial ancestors were the first living things to appear on Earth. The First observations: One of the most important discoveries in the history of Microbiology occurred in 1665
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