How to Carry Out Aseptic Techniques in a Batch Culture and in the Laboratory | | | | | | | | | |The batch vessel should be sterilised beforehand using steam. The nutrient medium that is added to the vessel |
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Microbiology is the study of living organisms that are too small to be clearly seen by the unaided eye. The science of Microbiology dates back only to 200 years‚ yet the recent discovery of Mycobacterium tuberculosis DNA in 3000 year old Egyptian mummy reminds us that microbes have been around for much longer. Infact‚ Bacterial ancestors were the first living things to appear on Earth. The First observations: One of the most important discoveries in the history of Microbiology occurred in 1665
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more bacteria and have a larger inhibition zone‚ than Proactive™ will. Experimental Design Materials Needed for this experiment‚ is a container of Proactive™ and a container of Clearasil™. Some tools needed are: a Bunsen burner (aseptic technique to keep hands sterile when sanitized)‚ three sterile agar plates‚ a sharpie‚ sterile cotton swabs‚ two glass plates‚ tweezers‚ and sterile disks. Other materials needed are: Escherichia coli‚ Streptococcus epidermis‚ distilled water‚ ninety five
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* Microbiology: Study of microorganisms Most microorganisms are pathogenicFalse The majority of bacteria are harmless True Total number of bacterial species: about 10 million to a billion What is a microorganism? An organism or virus too small to be seen without a microscope. (Smaller than 0.5 mm) Ex: Bacteria‚ Fungi‚ Protozoa‚ Algae‚ and Viruses Microbiom: collection of microbes Human cells vs. Microbial cells = 1:10 Which statement about Michaelangelo is not correct? A. He was
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Culture Techniques In this first lab‚ you will be learning some very fundamental and important techniques. As is the case with most things‚ shorts cuts usually get you in trouble. This is especially true in Microbiology. The techniques you will be learning tonight‚ if mastered correctly‚ will make your life and learning experience in Microbiology much easier‚ if you don’t pay attention and practice these techniques incorrectly‚ well then……? Today you will be learning the following techniques: 1.
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Disposition/Food Safety: Overview of Food Microbiology July 8‚ 2011 Overview of Food Microbiology OBJECTIVES At the end of this module‚ you will be able to: 1. Explain the structural similarities and/or differences among Gram-positive and Gram-negative bacteria as well as their isolation and identification using serological‚ biochemical‚ and molecular techniques. 2. Identify the functions of the bacterial cell wall. 3. Identify the extrinsic and intrinsic parameters that affect bacterial growth
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SCOPE OF MICROBIOLOGY: What is Microbiology? | � Microbiology is the study of microorganisms� Microorganisms‚ roughly‚ are living things that are too small to be seen with the naked eye� Microorganisms cannot be distinguished phylogenetically from �macroorganisms� (see variety of microorganisms‚ below)� Microbiology is more a collection of techniques: aseptic technique‚ pure culture technique‚ microscopic observation of whole organisms‚ etc.� Microbiologists
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Chapter 1: The Main Themes of Microbiology What is Microbiology? • micron = small and biologia = study of living things • Microbiology = study of small living things • How small is “small”? • What do we really mean by living? • Working definition: Study of entities too small to be seen with the unaided human eye. • (< 0.2 mm = 200 µm = 200‚000 nm) Types of Microbes Prokaryotes include Bacteria and Archaea Eukaryotes include Protista (Algae and Protozoa)‚ Fungi‚ and Animalia
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Microbiology Unknown 5/7/2013 Microbiology Unknown #94 The purpose of this report was to identify an unknown bacterium by applying methods that were previously learned and conducted in microbiology lab class. It is possible to identify a mixed culture by running experiments on the unknown bacteria and taking what we already know and applying it to the results. It helps to slowly eliminate any bacteria that do not correspond with the results of certain tests. An unknown test tube labeled 94 was
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MICROBIOLOGY What is the purpose of general growth media‚ selective media‚ and differential media? Give examples of each. General Purpose Media General Purpose media is designed to grow most organisms and do not contain growth inhibitors. Standard Methods Agar and Blood Agar Bases are examples of general purpose media. Differential Media Differential media contain a component that allow an observable change when a specific chemical reaction takes place. Simmons Citrate Agar is an example
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