NUMBER TWO DATE: 3/25/2010 inal attachment Lab Experiment number 11 PURPOSE: To learn the Gram stain technique‚ the reason for the stain‚ and how to identify the results of the organisms stained. MATERIALS: Bunsen burner‚ inoculating loop‚ staining tray‚ glass slides‚ bibulous paper‚ lens paper‚ oil‚ and microscope METHODS: Apply Crystal Violet (Primary stain) for 1 minute. Rinse with D-water Apply Iodine (Mordant) for 1 minute. Rinse with D-water. Apply Alcohol (Decolorize) for 30 seconds
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Microbiology Lab Quiz #2 Study Guide Exercise 9 -- The Gram Stain Compare and contrast simple and differential staining techniques. Simple Staining Simple staining is useful in determining the basic morphology of an organism. Simple staining involves only one reagent i.e. crystal violet‚ basic fuchsin‚ or methylene blue. Simple dyes used to stain bacteria have a positive charge cationic (+)‚ therefore‚ they are attracted to the bacteria that are usually negatively charged anionic (-) Depends
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Tortora‚ Funke‚ Case (2010). Microbiology : An introduction‚10th Questions 1. From the procedure above‚ can you determine the intracellular location of the spore? Can this also used for taxanomic purposes? When viewed unstained‚ endospores of living bacilli appear edged in black and are very bright and refractile. Endospores strongly resist application of simple stains or dyes and hence appear as nonstaining entities in Gram-stain preparations. However‚ once stained‚ endospores are quite resistant to
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NTRODUCTION Often in the study of microbiology‚ the need to identify an unknown microbe may arise. This aids in the recognition of which microbes are harmful or pathogenic as well as those that may be beneficial to us in some way. This report will detail how a similar project was performed in order to identify two unknown micobes using the techniques that were learnt during the course of the semester. We embarked on the project with the knowledge that we would have to identify a Gram(+) as well
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bacterium were taken after 48 hours of incubation at 35° Celsius. Microscopic observations were performed on four different microscope slides. Three of these observations were gram stains and the last observation was of an endospore staining. The unknown bacteria samples for the endospore stain were taken off of a nutrient agar plate and stained using the Bartholomew and Mittwer’s method. Gram stain samples were taken off of tryptic soy agar and stained using the gram stain procedure that is listed in
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B.Sc. First Year Semester – I Paper I .Fundamentals of Microbiology Credit - 1 1 Scope & relevance of Microbiology i) ii) iii) Definition & concepts Types of microorganism Distribution of microorganisms in nature 2. Development of Microbiology as a Scientific Discipline 1.1 History i) ii) iii) iv) v) vi) Credit – 2 Early observation of microorganisms Spontaneous generation conflict : Contribution of scientists Recognition of the microbial role in diseases. Koch’s postulates Recognition of microbial
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Exercise 8-B Differential Staining Gram Staining and Acid Fast Staining Introduction: Differential Staining‚ one which facilitates differentiation of various elements in a specimen is a general term that can refer to a number of specific processes. Using multiple stains can better differentiate between different microorganisms or cellular components of a single organism. Gram’s Stain is a widely used method of staining bacteria as an aid to their identification. It was originally devised by Hans
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Study Guide to Help with Laboratory Exam 1 Microbiology 1214 A partial study guide only. Not intended to replace the review sheets or the “Know the following” sections on your labs. These slides are not presented in any particular order. Name this Procedure Identify the two types of bacteria present by shape and gram stain. In a gram stain what is the primary stain? The mordant? The counterstain? How does this differ from a simple stain? Identify the shape and gram stain of the bacteria present
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Section 4 Biol 311 Staining and Identifying Unknown Bacteria Introduction: The microbiology lab up to this point has been used to teach the students how to stain and identify bacteria. There are several types of staining through which the bacteria can be identified based on the color and shape. The staining methods used in the lab are Gram Staining‚ Capsule Staining‚ Endospore Staining‚ and Acid Fast staining. One of the most significant method of staining is the Gram Staining‚ as it is highly dependent
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and food products may become contaminated‚ leading to the transient colonization of the human intestine. Illness associated with B. cereus can occur when heat-resistant B. cereus endospores survive cooking. If the food is then inadequately refrigerated or held for extended periods at improper temperatures the endospores can germinate and multiply. Once the spores germinate‚ the vegetative cells can multiply and produce illness causing enterotoxins. Bacillus cereus is known to cause two distinctly
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