shapes would distort. BM SC Negative Stain CULTURES: Bacillus subtilus Staphylococcus epidermis MATERIALS: Nigrosin‚ Clean slide‚ Distilled water‚ Sterile toothpicks FUNCTION: This technique is very useful in situations where other staining techniques do not show clear morphology or size. PROCEDURE: 1. Place a small drop of nigrosin near on end of the slide. Mix a loopful of broth culture in the drop. (When from a solid medium‚ add a loopful of water) 2. Draw a second slide
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2013). Gram positive bacteria occupy a multilayered thick peptidoglycan‚ and teichoic acid. Teichoic acids assist with distinguishes gram positive bacteria from other bacteria. The Gram positive bacteria are easier to kill. The gram reaction of dye staining is blue or violet. Gram-negative bacteria cell wall can be described as having a thin peptidoglycan‚ no teichoic acid but it does have an outer membrane lipopolysaccharide‚ lipoproteins‚ and phospholipids. The lipopolysaccharide is on top of the
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Microbiology: study of small living things study of bacteria: bacteriology study of viruses: virology study of parasites: parasitology study of fungi: mycology NOT ALL BACTERIA ARE PATHOGENS (disease causing) antibiotic: used to treat bacterial infections and diseases (penicillin‚ mold‚ 1929) bioremediation: using bacteria to clean up toxins pathogenesis: ability of an organism to be pathogenic virulence factors: toxins‚ receptors‚ cellular composition innate immune response v.s
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SCI250 Week 1 Chapter 3 Staining Lab Quiz Section: Multiple Choice 1. Which of the following stains is used frequently to identify Mycobacterium and other bacteria whose cell walls contain high amounts of lipids? A. Gram stain B. Schaeffer-Fulton stain C. Acid-fast stain D. Lipidialar stain E. Spore Stain 2. Which of the following stains is used to classify microorganisms based on their cell wall content? A. Capsular stain B. Gram stain C. Spore stain D. Negative stain E. Methylene blue 3
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for bioremediation? Curing infectious diseases - All of the following are examples of new emerging infectious diseases except Chickenpox - Normal microbiota: Beneficial microbial inhabitants of the body - Golden Age of Microbiology: Rapid discovery of basic microbiology principles - Re-emerging diseases: Once controlled by preventative public health measures they are now on the rise - Prions: Resistant to the usual sterilization procedures for pathogens - Spontaneous generation: The converse
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experiments under categories‚ such as‚ morphology‚ physiology‚ antibacterial susceptibility‚ selective media‚ and biochemical provide results. Both the unknown isolate and members of the Micrococcus genus were shown to be obligate aerobes. By using staining methods‚ this proved that the organism is gram positive. Morphology‚ such as‚ orange pigmentation and coccus shape provide similarities to the Micrococcus genus. Physiological tests were shown to be obligate aerobe‚ mesophile‚ neutrophile‚ and osmotolerant
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fix the smear. Simple Staining = staining cells with a single dye so that they can be more readily observed. Basic Dye = (methylene blue) has positively charged chromophore group. A basic dye will be attracted to any negatively charged substance‚ such as bacterium. Acidic Dye = (eosin) have negatively charged chromophore groups‚ and thus are attracted to positively charged substances. Chromophore Group = chemical staining group. Bacilli = rod- shaped bacterium. Endospore = survival forms of the
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Control of Microbial Growth: definitions Sterilization: Killing or removing all forms of microbial life (including endospores) in a material or an object. Heating is the most commonly used method of sterilization. Commercial Sterilization: Heat treatment that kills endospores of Clostridium botulinum the causative agent of botulism‚ in canned food. Does not kill endospores of thermophiles‚ which are not pathogens and may grow at temperatures above 45oC. Control of Microbial Growth: definitions
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if the unknown organism is a Gram positive or Gram negative. This is the initial step that must be taken before any other lab procedure may continue on to ensure the purity is present‚ the arrangement of the cells‚ and the shape the cell has. The staining of the cell starts off with using the primary stain‚ Crystal Violet which is a purple color‚ to begin with. Next‚ Gram Iodine is added on‚ which is our mordant and this causes a complexion with the primary stain‚ results in formation of insoluble
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Clinical Microbiology Milena Ivakovic Western Governors University Bacterial Morphology Task 11 Clinical Microbiology A: In wet mount stain‚ color of the slide is same gray‚ colorless and glossy. Liquid or fluid appearance is visible. Bacteria and background don’t have a much of difference in color. Although‚ bacteria are defined and visible noted. Most of the time they are single round cells. In direct stain with crystal violet‚ again background is colorless and glossy. Bacteria is blue and some
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