agent of a disease in a patient‚ so as to know how it can be treated‚ to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium. Broth culture #72 was randomly selected and subjected to qualitative tests for identification. It is suggested that culture #72 is an example of Serratia marcescens and Micrococcus
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Clostridium Difficile | | Clostridium Difficile is a Gram-positive‚ toxin-producing anaerobic bacterium belonging to the family Clostridiaceae of the Clostridiales. Though strictly oxygen-intolerant‚ C. Difficile is able to produce aero tolerant endospores under unfavorable conditions that are capable of persisting in an open environment for years. C. Difficile is a commensalist species typically housed in the colonic fecal flora of a fairly small subset of the child population‚ with the number of
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Alyse Rose Microbiology Lab Bacterial Unknown March 25‚ 2013 Bacterial Unknown Report Each student was given an unknown bacteria to figure out. I was given the unknown bacteria S38. Everybody is supposed to do all sorts of test to identify the bacteria. The first thing I did was smear my bacteria on a liquid medium. I then proceeded to incubate the medium for 24-48 hours. 1. GRAM STAIN The next step I took in finding my unknown bacteria was to gram stain it. This is used to differentiate
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Multiplication of contaminant bacteria in urine and interpretation of delayed culture. Abstract A prospective study of the bacterial populations of non-infected urine was mounted in an attempt to define the length of delay between voiding and analysis during which culture would not give false positive results due to the multiplication of contaminant bacteria present at the time of voiding. The findings suggest that culture of urine within four hours of voiding is likely to give a true indication
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Aseptic Technique and Culturing Microbes Lab 3 Brittney Case August 4th 2013 Section: Mic 101 Microbiology Session 1 Abstract: The Purpose of this experiment is to gain knowledge of how to properly use aseptic techniques to transfer cultures‚ learning about cultured media and how to distinguish various types of microbial growths as well as what is required for them to grow properly. Hypothesis: This exercise will allow me to gain knowledge of how to properly use aseptic techniques
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Bacterial Biology █ BRIAN D. HOYLE An understanding of the fundamentals of bacterial biology is critical to bacteriologists and other forensic investigators attempting to identify potential biogenic pathogens that may be exploited as agents in biological warfare or by bioterrorists. Fundamentals of Bacterial Biology Bacteria are one-celled prokaryotic organisms that lack a true nucleus (i.e.‚ a nucleus defined by a membrane). Bacteria maintain their genetic material‚ deoxyribonucleic
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Microbiology Kit (MBK) – Lab Report Name: Section: Observing Bacteria and Blood Questions: A. List the following parts of the microscope and describe the function of each A. Eyepiece lens B. Tube C. Revolving nosepiece/turret D. Objective lenses E. Stage F. Iris Diaphragm G. Illuminator H. Coarse Focus I. Fine Focus J. Arm K. Stage Clips B. Define the following microscopy terms: a. Focus: A means of moving the specimen closer or further away from the
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a MacConkey agar plate. The first part of the experiment involved the methods of manipulating‚ identifying and counting the bacteria and the second part was to find out whether the bacteria E.coli was the only type found in the given area by gram staining. E.coli was the chosen bacteria for this type of experiment. It is a gram negative bacterium that will grow rapidly given ‘any culture medium with the necessary energy source‚ nutrients‚ pH‚ and temperature’. Therefore‚ MacConkey Agar being the
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related epidemiology has become increasingly more important. The purpose of this study was to identify an unknown bacterium in a controlled laboratory environment over a 5 week period. Utilizing a variety of differential testing and staining methods learned in the microbiology course‚ students were to determine the identity of an assigned unknown organism. Observations were made and recorded each week to narrow down the scope of identification. Data has been presented in the tables‚ charts and drawings
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Chapter 25 Pre-Test Question 1 Part A Miller and Urey’s experiments that attempted to recreate conditions on early Earth were significant because _____. Hint 1. They gave credence to the idea of abiotic synthesis. ANSWER: they showed how polymers could form spontaneously on a clay substrate they produced the first protocells they showed how radiometric dating could be used to give the absolute ages of rocks and fossils proved the endosymbiotic theory they showed that organic
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