MICROBIOLOGY What is the purpose of general growth media‚ selective media‚ and differential media? Give examples of each. General Purpose Media General Purpose media is designed to grow most organisms and do not contain growth inhibitors. Standard Methods Agar and Blood Agar Bases are examples of general purpose media. Differential Media Differential media contain a component that allow an observable change when a specific chemical reaction takes place. Simmons Citrate Agar is an example
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Chapter 1. Microbiology – Diversity of Organisms Microorganisms- too small to be seen with the unaided eye “germ”- rapidly growing cell Microbes in our lives Pathogenic- disease causing Decompose organic waste Producers in ecosystem (photosynthesis) Produce industrial chemicals such as ethanol and acetone Produce fermented foods ( vinegar‚ cheese‚ bread) Produce products used in manufacturing (cellulose) and treatment (insulin) Designer Jeans: Made by Microbes? Stone washing- Tricoderma
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(2005). Evidence for functional overlap among multiple bacterial cell division proteins: compensating for the loss of FtsK. Mol Microbiol 58: Pages 596–612 Gordon‚ G.S Grainge I. (2010). FtsK – a bacterial cell division checkpoint? Molecular Microbiology Volume 78‚ Issue 5‚ pages 1055–1057 Grainge I.‚ Lesterlin C Griffiths A. J. F.‚ Wessler S. R.‚ Lewontin R. C. and Carroll S. B. (2008). Introduction to Genetic Analysis. W.H. Freeman and COmpany. Chapter 7: DNA: Structure and Replication. pages
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Lab Practical 2 Morphological Staining Techniques Simple Stain- Uses 1 Stain 1. Acid Stains (- Charge)- Stains Background- Nigrosin‚ India Ink and Congo Red 2. Base Stains (+ Charge)- Stains Cell- Methylene Blue‚ Crystal Violet‚ Safranin Differential Staining Techniques- Any Staining Technique using 2 or more stains is differential. It allows us to differentiate between parts. 1. Gram Stain- Two Stains‚ PLUS Reagents- Distinguishes Chemical Composition of Cell Wall PG only (+ Purple)
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Experiment 2: Microscopy & Simple Staining September 30‚ 2013 General microbiology laboratory The invention of the microscope has opened up a whole new dimension in science. By using microscopes scientists were able to discover the existence of microorganisms‚ study the structure of cells‚ and see the smallest parts of plants‚ animals‚ and fungi. Today‚ the microscope is still a commonly used tool to diagnose illness in hospitals and clinics all over the world (1)
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SCOPE OF MICROBIOLOGY: What is Microbiology? | � Microbiology is the study of microorganisms� Microorganisms‚ roughly‚ are living things that are too small to be seen with the naked eye� Microorganisms cannot be distinguished phylogenetically from �macroorganisms� (see variety of microorganisms‚ below)� Microbiology is more a collection of techniques: aseptic technique‚ pure culture technique‚ microscopic observation of whole organisms‚ etc.� Microbiologists
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Microbiology Laboratory Report Identification of Unknown Bacteria 6O BIO 251-7H1 Gram Stain Summary: Out of 28 Unknown microorganisms‚ slant 6O was randomly selected‚ and subjected to testing in order to identify it’s species. Seventeen of the unknown bacteria were classified as Gram-negative bacilli: Corynebacterium pseudodiphtheriticum‚ Enterobacter aerogenes‚ Eschirichia coli‚ Flavobacter capsulatum‚ Helicobacter pylori‚ Klebsiella pneumonia‚ Klebsiella oxytoca‚ Proteus hauseri‚ Proteus
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BTN 211 TOTORIAL 1 Chapter 1 1. The History and Scope of Microbiology Which of the following distinguish the field of microbiology from other fields of biology? a. the size of the organism studied b. the techniques used to study organisms regardless of their size c. both the size of the organism studied and the techniques employed in the study of organisms d. neither the size of the organism studied nor the techniques employed in the study of organisms regardless of their size Who of
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Microbiology is the study of living organisms that are too small to be clearly seen by the unaided eye. The science of Microbiology dates back only to 200 years‚ yet the recent discovery of Mycobacterium tuberculosis DNA in 3000 year old Egyptian mummy reminds us that microbes have been around for much longer. Infact‚ Bacterial ancestors were the first living things to appear on Earth. The First observations: One of the most important discoveries in the history of Microbiology occurred in 1665
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and therefore necessary during cellular proliferation. The protein can be found in all phases of the cell cycle but not in resting cells. The Ki67 staining is used to determine growth of certain cell types and is therefore used on hyperproliferative cells such as tumor cells‚ but also in benign hyperproliferative diseases such as psoriasis. The staining reveals the proliferative active keratinocytes which can be found in stratum basale. Sept7-deficient mice receiving IMQ treatment (Fig S6. A) had
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