Amanda Lab Report 1 Microbiology Laboratory Title – Differentiating Organisms using the Gram Stain Introduction The experiment conducted was based upon the known attributes of two different groups of bacteria‚ those that are gram positive‚ and those that are gram negative. Using a specific staining procedure‚ it is possible to differentiate the two types under a microscope The gram stain method of differentiation is possible because of differences in the cell membrane between the two categories
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(2005). Evidence for functional overlap among multiple bacterial cell division proteins: compensating for the loss of FtsK. Mol Microbiol 58: Pages 596–612 Gordon‚ G.S Grainge I. (2010). FtsK – a bacterial cell division checkpoint? Molecular Microbiology Volume 78‚ Issue 5‚ pages 1055–1057 Grainge I.‚ Lesterlin C Griffiths A. J. F.‚ Wessler S. R.‚ Lewontin R. C. and Carroll S. B. (2008). Introduction to Genetic Analysis. W.H. Freeman and COmpany. Chapter 7: DNA: Structure and Replication. pages
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virus too small to be seen without a microscope (smaller than 0.5mm) Misconceptions of Microorganisms * All microorganisms are pathogenic (disease producing) FALSE * The majority of bacteria are harmless and beneficial TRUE Aspects of microbiology * Basic biological science * About living cell and how they work * About microbial diversity and their evolution * Applied biological science * What microorganisms do; their role in the nature? * Causative agents
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Review Questions 1. Which type of S. pneumoniae strain is the most virulent‚ encapsulated or unencapsulated? 2. What allows S. pneumoniae to adhere to cell walls‚ phosphorylcholine or polysaccharide? 3. Which infections are most commonly caused by S. pneumoniae‚ arthritis & peritonitis or meningitis & bacteremia? both 4. What can society do to help protect itself from antimicrobial resistance‚ increase or decrease antimicrobial usage? Decrease usage 1. Staphylococcus aureus‚
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Microbiology: An Introduction‚ 10e (Tortora et al.) Chapter 14 Principles of Disease and Epidemiology Test Bank 1) A commensal bacterium A) Does not receive any benefit from its host. B) Is beneficial to its host. C) May be an opportunistic pathogen. D) Does not infect its host. E) B and D only. Answer: C media. B) Some microorganisms don’t cause the same disease in laboratory animals. C) Some microorganisms cause different symptoms under different conditions. D) Some microorganisms can’t be observed
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Medical Mycology: Yeast and Pneumocystis| Reading Assignment:|Mahon‚ Chapter 10‚ pgs 215-219‚ Chapter 27‚ pgs 626-629‚ 634-636‚ Appendix B Lecture Notes: Medical Mycology| |U of W Tutorial on Mycology (organisms listed in objectives)‚ www.medtraining.org[->0]| _____________________________________________________________________ 1. Discuss the difference between yeasts and molds. Fungi seen in the clinical laboratory can be generally separated into two groups based on the appearance of the
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SCOPE OF MICROBIOLOGY: What is Microbiology? | � Microbiology is the study of microorganisms� Microorganisms‚ roughly‚ are living things that are too small to be seen with the naked eye� Microorganisms cannot be distinguished phylogenetically from �macroorganisms� (see variety of microorganisms‚ below)� Microbiology is more a collection of techniques: aseptic technique‚ pure culture technique‚ microscopic observation of whole organisms‚ etc.� Microbiologists
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you in trouble. This is especially true in Microbiology. The techniques you will be learning tonight‚ if mastered correctly‚ will make your life and learning experience in Microbiology much easier‚ if you don’t pay attention and practice these techniques incorrectly‚ well then……? Today you will be learning the following techniques: 1. Streak plate method for colony isolation 2. Aseptic transfer techniques First the streak plate methods. In Microbiology it is often necessary to isolate pure colonies
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performed. First‚ a gram stain was performed‚ indicating the bacterium was gram negative. An aerotolerance test determined that the bacterium was a facultative anaerobe. Next‚ a negative result in the methyl red test indicated that no mixed acid fermentation occurred. The DNase test was performed and yielded a positive result. The SIM test provided two outcomes‚ that the bacterium did not reduce sulfur nor produce indole from tryptophan. Afterwards‚ the bacterium was determined to be positive for lysine decarboxylation
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Microbiology in odontogenic infections Most odontogenic infections contain mixed aerobic and anaerobic bacteria. predominant bacterial species present in oral cavity are maunly Streptococcus ‚ Peptostreptococcus‚ Veillonella‚ Lactobacillus‚ and Actinomyces . 11‚12 The mixed aerobic-anaerobic composition of the bacteria involved in suppurative odontogenic infections is thought to be important in the pathogenesis of infection. if bacteria involved in mixed odontogenic infections are isolated in pure
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