T-φ gram Determination of Unreported Meteorological Quantities from T-φ gram 1. Mixing Ratio (W):- It is the ratio of the mass of water vapour to the mass of dry air containing the vapours at a certain temperature. It is expressed in gm/kg. On T-φ gram from dew point temperature we follow isohygric doted green line and read the value at the base. (It can be calculated at all levels where dew point temperature is available). 2. Saturation Mixing Ratio (Ws) :- Mixing ratio at
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Part B: Gram Stain Preparation: Watch the following video: The Gram Stain (4:15 minutes) Procedure: Include the answers to the questions below in your lab report. Lab Questions: This procedure is used to separate what two types of bacteria? This separation is based on differences in what physical trait? What is the iodine used for in this staining technique? Why is it important to heat fix the bacteria prior to the staining procedure? If you had a bacteria sample that you knew to be gram positive
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from those of the host; thereby showing little or no effect to the host. 2. What are broad and narrow spectrum antimicrobials? What are the pros and cons of each? Broad spectrum antibiotics work against many types of organisms such as gram positives‚ gram negatives and anaerobes. The great thing about this is that these are prescribed empirically so that all potential bacteria are covered until the culture results return. The problem with the misuse of broad spectrums is the resistance it can
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Gram Stain: The important part of this experiment is being able to determine a bacterium based on its cell wall structure. It also helps indentify if the unknown organism is a Gram positive or Gram negative. This is the initial step that must be taken before any other lab procedure may continue on to ensure the purity is present‚ the arrangement of the cells‚ and the shape the cell has. The staining of the cell starts off with using the primary stain‚ Crystal Violet which is a purple color‚ to begin
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Lahela Correa 12/08/2009 Microbiology 140 Matthew Tuthill Unknown Lab Report Introduction There are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient‚ so as to know how it can be treated‚ to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that I have been learned so far in the microbiology laboratory class for the identification
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the unknown organism included Gram Staining‚ Fluid thioglycollate/aero-tolerance test‚ Methyl Red-Vogues Proskauer‚ SIM test‚ Lysine Decarboxylase test‚ Lactose Fermentation test‚ Simmons Citrate test‚ and TSI test. The Gram staining technique showed that the organism was Gram negative and bacilli shaped. The fluid thioglycollate test showed that the organism was a facultative anaerobe‚ meaning ATP can be produced through aerobic respiration or fermentation. Positive results for the remaining tests
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Chapter 1: The Main Themes of Microbiology What is Microbiology? • micron = small and biologia = study of living things • Microbiology = study of small living things • How small is “small”? • What do we really mean by living? • Working definition: Study of entities too small to be seen with the unaided human eye. • (< 0.2 mm = 200 µm = 200‚000 nm) Types of Microbes Prokaryotes include Bacteria and Archaea Eukaryotes include Protista (Algae and Protozoa)‚ Fungi‚ and Animalia
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Importance of Microbiology| in Nursing Agriculture Pharmacy Advertisement Microbiology is a subject dealing with microbes and related concepts. Microbiology has come a long way since discovery of microbes and is presently of great help to mankind. It is used in health care‚ food production‚ diagnosis‚ production of alcohol‚ maintenance of sterility and cleanliness etc. Though the subject was initially limited to study of microbes and their characteristics or properties‚ latter it was explored to
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A Basic Understanding of Microscopy Assignment 1 Kaplan University-Microbiology Since microorganisms are not visible to the eye‚ the essential tool in microbiology is the microscope. One of the first to use a microscope to observe microorganisms was Robert Hooke‚ the English biologist who observed algae and fungi in the 1660s. In the 1670s‚ “Anton van Leeuwenhoek‚ a Dutch merchant‚ constructed a number of simple microscopes and observed details of numerous forms of protozoa‚ fungi‚ and bacteria”
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Microbiology Final exam Study reference 1. Gram Stain * Verify if bacteria are present or not. * Controls – positive (purple) – S.aureus negative ( red/pink) – E.coli 2. Endospore Stain Positive controller – B. magneterium Green spore- Positive Pink (vegetative ) – Negative 3. Acid fast Positive control – M. smeagmatis Blue – negative Pink /red- positive 4. Motility Positive control - P.vulgaris A. Non motile is negative test B. Motile is a positive test 5. Carbohydrates
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