Pseudomonas taiwanensis – Enrichment‚ Isolation and Identification Microbiology 521 2/10/12 Purpose: The purpose of this experiment was to enrich Pseudomonas bacteria‚ isolating a species of Pseudomonas and identifying it using phenotypic properties and DNA sequencing as an existent or completely new and undiscovered species of Pseudomonas. Overview: Genus Pseudomonas is a chemoheterotrophic bacteria found in soil and water. They are Gram negative‚ motile‚ paired rods that are also oxidase-positive
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The properties of dibenzalacetone make it a great substance to be used when protecting the skin from the sun. In the experiment performed‚ dibenzalacetone was created from an aldol condensation of 2 portions of benzaldehyde and acetone in sodium hydroxide. The reaction that occurred in this experiment is known as a specific aldol condensation called Claisen-Schmidt. In the reaction‚ the carbonyl of benzaldehyde reacts quickly with the acetone anion to create a hydroxyketone that dehydrates in the
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Wanni Lin Biology 110 March 2‚ 2015 DNA Lab BACKGROUND In this laboratory experiment‚ students were introduced to DNA electrophoresis. DNA electrophoresis is an instrument that many forensic scientists use to get a DNA fingerprint as an evidence for crimes. Not only can it be used for forensic science‚ people can use this for paternity test‚ as well as look for evolutionary relationships among organisms. Agarose is used to make the gel that the DNA fragments are going into. Since DNA particles are
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Fly Lab Shannon Ladd Introduction: Famers and herders have been selectively breeding their plans and animals to produce more useful hybrids for thousands of years. It was somewhat of a hit or miss process since the actual mechanisms governing inheritance were unknown. Knowledge of these genetic mechanisms finally came as a result of careful laboratory breeding experiments carried out over the last century and a half. A contributing geneticist named Gregor Mendel (1822-1884)‚ discovered through
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successfully synthesis the reactant to the product‚ NaBH4 was used as the main reagent to reduce the carbonyl double bond. One believes the formation of isoborneol was successfully due to the product’s percent yield‚ IR‚ and melting point. During the lab‚ .077 grams of isoborneol was yielded from the camphor reduction. In result‚ .077 g compared to an theoretical yield of .102 g equaled an overall 75% percentage yield. Considering the product yield was only a quarter shy of a 100% yield‚ provides strong
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Grade 11 Dynamics Lab Report Friction SPH3U1-02 Williams. C. By----James & Hao Feng & Henry Zhang Purpose: By measuring the friction and μ of a container and change different variables including mass‚ surface and gradient‚ get causes of the change of friction and μ. Materials: A container Three Pen bag in different mass A rough wood board A clean desk Rulers Thrust meter. Steps Prepare
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Objective The purpose of this lab is to determine the particle size distribution of the fine and coarse aggregates by sieving. Equipment and Material Balance‚ sensitive to within 0.1% of the weight of the sample to be tested Standard sieves for grading of fine aggregates- 4.75 mm‚ 2.36 mm‚ 1.18 mm‚ 300m‚ 150m (# 4‚ 8‚ 16‚ 50 and 100) Standard sieves for grading coarse aggregates- 1 ½ in.‚ 1 in.‚ ¾ in.‚ ½ ‚ 3/8 in.‚ 4 in‚ plus a 4.75 mm(#4 sieve) Fine (0.5 Kg) and coarse (2 to 20 Kg depending
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bacteria will be one of the following: Enterococcus faecalis‚ Staphylococcus saprophyticus‚ Escherichia coli‚ Enterobacter aerogenes‚ Proteus vulgaris‚ Salmonella [I assume typhimurium]‚ or Shigella [either flexneri or sonnei‚ we used both in our lab during the semester]. Procedure {and observations}: Observe bacterial colony morphology. {Colonies are large‚ beige or cream-colored‚ with irregular borders.} Prepare two slides for gram staining and viewing under a microscope. {Either my gram-stain
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ABSTRACT: This lab allows us to observe the conversion of hydrogen peroxide (H2O2) into water and oxygen gas. An enzyme known as catalase facilitates this decomposition reaction. The catalase enzyme acts as catalysis‚ helping lower the energy needed to activate the reaction while the enzyme itself is not affected. Catalase is a digestive enzyme used to break down hydrogen peroxide‚ which is a normal byproduct of cellular respiration. The reaction could take place without the help of catalase‚ but
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salt solution is 6.3grams and the 15% salt solution is 6.8 grams. My hypothesis was that the carrot would shrink and be very small and gooey‚ but it was wrong. The controlled variable in this lab is the carrot and time‚ the manipulated variable is the salt solution‚ and the resulting variable in this lab is what happens to the carrot. Materials and Methods: . Salt solution ( 0-15%) . 4 Carrot pieces from same Carrot( Cut carrot in half if needed) . 4 Beakers . Water . Triple beam balance
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