Coli bacteria. We began the lab by first obtaining two sterile microcentrifuge tubes‚ one was label “GFP+” while the other one was “GFP–” because this one served as a control meaning nothing goes inside this one. With gloves on 25 microliters competent cells‚ which is e. Coli bacteria compete in calcium chloride and heat shock was added to each of the microcentrifuges. Eventually‚ both
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Laboratory Report The Plasma Membrane I. Introduction The Plasma membrane is the edge of life‚ the boundary that separates the cell from its surroundings. It controls the traffic of materials in and out of the cell. (Reece‚ 2011). It is incredibly thin that is very vital in maintaining the integrity of the cell. Not only does the plasma membrane bind the other organelles‚ it also forms a dynamic structure which gives them their remarkable activity and selectivity. (Hickman‚ 2008). Diffusion
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PEES 4200W- Physiology of Exercise Lab #2: Metabolism and Energy Expenditure 1. The Respiratory exchange ratio is the ratio of the volume of carbon dioxide produced and oxygen consumed. The ratio indicates the energy that the subject is expending for indirect calorimetry‚ how efficient the subject ’s body is at utilizing the oxygen inhaled‚ as well as the main substrate being used for energy during varying intensities of exercise. During rest the volume of carbon dioxide was 0.73L/min‚ the volume
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Session #1 Lab 1 OBSERVING BACTERIA AND BLOOD D Haynes BIO-218A Microbiology Lab Friday‚ January 17‚ 5:00pm Objective(s): Our objective is to gain knowledge of the functions and operations of the microscope through observation of bacteria and blood slides that are available for this assignment. We are also learning to “see” and observe various bacteria and their shapes as well as live yogurt cultures‚ blood‚ plaque and cheek swab samples and their arrangements. We
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Preliminary Unknown Report Alexis Grettenberger 800503431 Katie Young Alexis Grettenberger Biol 311 Lab 11/21/14 Preliminary Unknown Report Purpose: The purpose of this experiment was to identify a specific unknown bacterium that was isolated from a multitude of bacteria in a sample of contaminated dirt by running it through a series of identification techniques. Some of these techniques were simple staining‚ gram staining‚ and isolation techniques. The hypothesis of this experiment was that
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prefers oxygen but it is not necessary for growth. Many of these microorganisms will grow at the top of the tube‚ but will also be dispersed throughout as well. B. Discuss the temperature requirements of the organisms (you grew) in this lab. Both Staphylococcus epidermis and Lactobacillus acidophilus are mesophiles. Mesophiles grow best between 25- 45 degrees Celsius (Alonzo‚ n.d.). In this experiment both bacteria have grown easily at a maintained room temperature of 26 Celsius. C. Discuss under
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Ch. 1 Prokaryotes = bacteria ‚ archaea Eukaryotes = Fungi‚ protozoa ‚ algae (Growth:: Mitosis有絲分裂) Bacterial cell → Bacillus(rodlike) ‚ cocus (spherical)‚ spiral (curved) *P.S. 有時會star-shaped or square → 出現既形式: pairs‚ chains‚ clusters cell walls of bacteria → peptidoglycan (carbohydrate & protein complex) cell walls of plant and algal cell → cellulose Growth::Binary fission = bacteria reproduce by dividing into two equal cells bacteria 吸收nutrients from dead / living organisms/ photosynthesis
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1. Introduction: The goal of this lab was to demonstrate the microbiology technique of serial dilutions and how they can effectively be used in experiments. E. coli cells were exposed to UV light for various amounts of time in order to asses the effect on growth and thereby mutations since this cell was modified to not have photolyse no uvr genes for DNA repair and thus can only use the SOS response. Each group then diluted the cells accordingly based on their UV exposure time and counted the cells
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Endospore and Capsule Cell Staining Allison Lui Partner: Mary Chagin BIOL-235 W07 Dr. Runco 2/10/15 Introduction The purpose of this lab is to learn what endospore and capsules are‚ and how to identify them under a microscope using capsule and endospore staining methods. Capsules are found only in select bacteria‚ and serve a protective purpose. Made out of sugars and proteins‚ they are antiphagocytic‚ which prevents other cells to engulf the bacteria through phagocytosis. It
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chemicals before use. Abide by specific warnings and directions. 3. Collect all materials needed for a procedure before proceeding. 4. Perform reactions under the hood when directed. Chemicals may be weighed and prepared at balance or lab tables‚ but tests should be carried out under the hood. 5.Acids and caustic chemicals are stored in the hood. Please do not take these chemicals from the hood. Procedure: PART 1: Metathetical reactions Precipitation reactions A1. Add a
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