The science of microbiology is all about microorganisms‚ what they do and how they work. Microorganisms are ubiquitous‚ and are a very important part of life everywhere since they have been around for billions of years. The two main themes associated with microbiology are (1) having an understanding of the nature and function of the microbial world‚ and (2) being able to apply that understanding of the microbial world so that the benefits to the Earth and humans are known(6). This lab specifically
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Jennifer Hauss March 4‚ 2015 Bacterial Transformation Lab Report Introduction In this lab‚ the goal was to transform the bacteria e-coli to glow in the dark (or under a black light). Four plates were set up with agar in them for the bacteria to feed on and grow. Changes were then made to the bacteria. One plate was the control plate‚ having only the LB or agar for the bacteria and negative pGLO‚ which is the liquid not containing the plasmid. This is the plate that was compared with the three
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Introduction to Microbiology Laboratory report №10 Physical factors affecting growth microbes: Temperature‚ pH and oxygen requirement. Student: Temirlan Aitbekov Lab partner: Kanat Sadykov Instructor: Alessandra Clementi‚ MD‚GP Lab date: 7/11/14 Due date: 14/11/14 Nazarbayev University Abstract: This experiment is directed
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Abstract:Conjugation is a natural occurring process that involves the transfer of DNA from one cell into another through a physical connection between the cells. In the following experiment‚ two strains of Escherichia coli bacterial cells (donor F’lac+strs and recipient F-lac-strr) underwent conjugation to produce a transconjugant strain (F’lac+strr). MAC plates and streptomycin were utilized to determine if conjugation had occurred. When plated‚ the donor colonies appeared red and the recipient
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Deaminase)‚ Lysine Decarboxylase‚ and Ornithine Decarboxylase. Colonial morphology on EMB was used to prove the identity of the bacteria. The unknown bacteria was #9. After performing the tests it is determined that the unknown bacteria is Escherichia coli. 3. Methods: All tests were performed using the aseptic technique to assure the cultures were pure and not contamination was present. The inoculation loops and straight wire stab needles were placed over the flame of a Bunsen burner and heated until
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2. Material and methods 2.1 Sample collection The following 14 samples of Staphylococcus aureus were used in this study. All samples were obtained from food contact surfaces at three food premises in University of Putra Malaysia (UPM). Samples were collected from the surface of each material according to standard swabbing techniques. The collection was carried out during weekdays (15:00-17:00) after finished the preparation of foods. Sterile swabs were removed from the coded test tubes that contain
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Material and methods Hosts ‚ plasmids and chemicals E. coli TOP10 strain was used for cloning and proliferation. E.coli BL21 DE3 was used as expression host cell. The pET 28a(+) plasmid was employed for gene expression. All chemicals were obtained from Merck Company (Germany). Codon optimization and gene synthesis Sequence encoding V-domain was obtained from Swiss-port‚ Uniprot KB and National Center for Biotechnology Information (NCBI) databases. 6x His-tag sequence was placed at N-terminal
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Escherichia coli and Staphylococcus albus Some bacteria’s require a certain amount of light in order to function properly and there fore survive. Escherichia coli is found in the gut and intestines of mammals. Staphylococcus albus is generally a skin dwelling bacteria Aim: To test whether the amount and type of light that the bacteria’s E.coli and S.albus are subject to causes changes in their growth rates and habits. To test whether the growth rates and habits of the bacteria’s E. coli and S
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Antibacterial Properties of Derris elliptica Benth. (Opay) Against Staphylococcus aureus and Escherichia coli A Special Problem Outline Presented to The Faculty of the Department of Biological Sciences College of Arts and Sciences Central Luzon State University In Partial Fulfillment of the Course Requirement in Animal Physiology (Bio255) For the Degree of Bachelor of Science in Biology October 2013 CHAPTER I INTRODUCTION Background of the study Derris eliptica (Wallich) Benth belongs
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E. coli Escherichia coli‚ more commonly known as E. coli‚ are a large group of bacteria. It lives in the digestive tracts of humans and also animals. There are many different types‚ such as enterohemorrhagic E. coli (EHEC) which causes bloody diarrhea. Some types cause urinary tract infections. One other specific type that is real common is called E. coli O157:H7. This type can cause severe anemia‚ kidney failure‚ or even death. The way you contract E. coli is coming into contact with feces from
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