E. Coli Transformation with Plasmid (pGal)‚ pGal Isolation‚ and Analysis of Plasmid DNA Felicia Osadi Bio 22 April 20‚ 2012 Transformation = group 10 Plasmid = group 7 RFLP = group 1 RESULTS Table I. Plasmid Transformation of E. Coli. Plate # | Agar plate | Type | Result | 1 | X-gal | Control | Extensive lawn growth | 2 | Ampr / X-gal | Control | Clear no bacterial growth | 3 | Ampr / X-gal | Transformation | 1 blue colony | Transformation efficiency = 1 transformants
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(Staudt 80). In this lab‚ solubility in water‚ acidity‚ and odors of carboxylic acids will be observed. Also‚ esters will be produce and odors will be detected from the product. Finally‚ saponification will be learned. PROCEDURE: Carboxylic Acids and Their Salts: Characteristics of acetic acid: 2mL of water and 10 drops of glacial acetic acid was placed into a clean‚ dry test tube. Before
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Radial Immunodiffussion (RID) Christian Crespo 18 October 2013 Immunology Lab Report Purpose of the Experiment: The objective of this experiment is to quantitatively observe the foundational reaction in our Immune system; the Antigen-Antibody interactions. The Ouchterlony procedure is what will be used in this lab to detect nature of the antibody interaction. The orientations of the band will provide more information about the interaction of antibody and antigen
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2014 Authored by: Cody Appleby‚ Gregory Harmeling‚ and Joseph Pernici May 26th‚ 2014 Lab section: C014 Monday 11am-1:50pm TA: Eduardo Castillo Work station 8 1 Abstract This lab consisted of three different measurements; measuring length‚ flow rate‚ and resistance. The reason for this lab was to use statistical concepts learned in lecture to analyze data and to become more familiar with the lab equipment. Repeatability measurements were taken on a bolt to get the total length‚ and
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Unknown Report Emily Juhl Section – 3 April 15‚ 2013 Unknown #8 Family: Enterobacteriaceae Genus: Serratia Species: Marcescens Abstract Microorganisms are constantly finding ways to resists to antibiotics. For this reason‚ it is important to test and observe unknown organisms in the lab to continually improve the health and well being of society. The objective of this report was to first isolate a single colony of an unknown culture on a LB agar plate. From
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Planarian Lab Report Hanna Klein Bishop Fenwick High School Planarian Lab Report Introduction Planarian worms are free-living flatworms that are not parasitic. Planarians have a three-cavity digestive system. Planarians are usually either carnivorous or scavengers. Planarians may eat other living‚ as well as dead‚ invertebrates‚ detritus or decaying organic matter. Planarians do not realize that are eating a lot‚ and can over eat resulting in death. Most planarians are freshwater forms that can
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Chromatography lab Purpose: To separate food colorings into their component dyes using paper chromatography. Materials: Chromatography paper‚ Food coloring‚ Ruler‚ Pencil‚ Solvent solution‚ Test tubes‚ Test tube rack. Safety precaution: wear aprons‚ to make sure that you don’t get any of the alcohol on your clothes‚ and if you break a test tube you don’t get glass on you. Procedure: See-attached handout. Results: See chromatography with Audrey’s lab report.
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Our purpose for this experiment is to observe how well organisms grow on three different media types such as chemical defined‚ complex‚ selective‚ and differential media. Experimental Methods In this lab‚ we use the procedure from Chemically Defined‚ Complex‚ Selective‚ and Differential Media. We divide the agar plates that have the unknown in third‚ write our initials‚ and date on the bottom of the agar plate. We also divide the agar plates into quadrants‚ label each organism in their quadrant
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Potato Osmosis and Diffusion Lab Report Emily Cocq Research Question: Will there be a difference in the experimented potato cores after being subjected to high concentrations of sugar? Introduction: Water can move through protein channels in cell membrane/cell walls. The water will move due to the water potential of the cells. If there is a greater concentration of solutes (chemicals) inside the cell than outside the cell and the chemicals can not move‚ then water will respond by moving
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[Name] [Teacher] [Course] [Date] Catalase Lab Report An enzyme is something that helps to speed up a chemical reaction. The enzyme changes from reaction to reaction‚ but it always has the same impact. However‚ certain variables may cause the enzyme to have a more or less significant impact on the speed of each reaction. One of these variables that changes the effectiveness of an enzyme is temperature. There is an optimal functioning temperature for each enzyme in each reaction‚ depending on
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