Laboratory Exercises in Microbiology‚ Fifth Edition Front Matter Preface © The McGraw−Hill Companies‚ 2002 PREFACE Take interest‚ I implore you‚ in those sacred dwellings which one designates by the expressive term: laboratories. Demand that they be multiplied‚ that they be adorned. These are the temples of the future—temples of well-being and of happiness. There it is that humanity grows greater‚ stronger‚ better. Louis Pasteur (French chemist‚ founder of microbiology‚ 1822–1895) There
Premium Microscope Microbiology Microorganism
Escherichia coli also known as E. Coli‚ is a type of bacteria which thrives in polluted water. Many people will come in contact with this type of bacteria in their lifetime. In fact‚ E. coli is normally found in the intestine of humans and animal where it plays a significant role in the digestive system. The bacteria can also be found in raw meat and uncooked vegetables as well as polluted water. Most of the strain of E. coli are harmless therefore they will not cause illness if consumed. “One of
Premium
In the experiment involving varying pH levels‚ E. faecalis‚ S. epidermidis‚ L. lactis‚ and L. casei were subjected to different pH levels and then were allowed to incubate in order to determine the minimum‚ maximum‚ and optimum pH levels for growth of specific bacterial species. It was found that lower pH levels between 2 and 4 inhibited or promoted little to no growth for E. faecalis‚ S. epidermidis‚ and L. casei. It is evident that these bacteria were resistant to alkaline environments‚ however
Premium Bacteria Escherichia coli Antibiotic resistance
LAB REPORT NUMBER TWO DATE: 3/25/2010 inal attachment Lab Experiment number 11 PURPOSE: To learn the Gram stain technique‚ the reason for the stain‚ and how to identify the results of the organisms stained. MATERIALS: Bunsen burner‚ inoculating loop‚ staining tray‚ glass slides‚ bibulous paper‚ lens paper‚ oil‚ and microscope METHODS: Apply Crystal Violet (Primary stain) for 1 minute. Rinse with D-water Apply Iodine (Mordant) for 1 minute. Rinse with D-water. Apply Alcohol (Decolorize) for
Premium Gram staining Staining Bacteria
1) TITLE (2pt): Testing antimicrobial props in soaps 2) INTRODUCTION (6 pts): In this experiment we wanted to test how effective different types of soaps against E. Coli. For this experiment we were interested in looking at antimicrobial soaps vs. non-antibiotics soaps in their effectiveness to kill E. Coli. The protocol that we used was the Kirby Bauer technique. This technique was used to test the antimicrobial properties of each soaps. (“Kirby-Bauer” 2015). 3) METHODS (6 pts): We first started
Premium Bacteria Escherichia coli Antibiotic resistance
This article is about public and private swimming pools being contaminated with E. coli. Chlorine is not doing its job in sanitizing the human waste in the swimming pool. Human waste seems to refuse being sanitized. The Centers for Disease Control (CDC) concluded that the E. coli came from one major primary source; swimmers pooping in the swimming pool. Researchers say such contamination is due to swimmers not rinsing themselves off before going in the pool. Scientists say a person carries about
Premium Swimming pool Chlorine
METHODS In this experiment‚ plasmid lux and a control plasmid (pUC18) will be introduced into E. coli by transformation. There are four basic steps to the procedure; Preparation of competent cells (These steps should be performed by the instructor.) 1. Place a vial of CaCl2 solution and the tube of E. coli in the ice bath. 2. Using a sterile pipet‚ transfer 590µL CaCl2 solution to the tube containing 50µL of the bacteria. 3. Tap the vial with the tip of your index finger to mix the solution. 4. Incubate
Premium Water Bacteria Chemistry
their food intake is through meat and dairy products and it’s not a lie when people say the food industry is a bunch of shady people. Past generations have been able to eat raw beef without even considering consequences like e-coli‚ but at this point in the United States e coli is the least of our worries. It is time to stand up against the food industry and make a change for the better of our world‚ and our future generations. The United States is a country where food is being mass-produced. We
Premium Milk
Department of Education Agusan National High School Caraga Administrative Region Division of Butuan City Antimicrobial Activity of Banana (Musa sapientum Linn.) Inflorescence Ethanolic Extract against E. coli and S. aureus Armona Jean B. Ongtico Mel Algil A. Agra Jerrson F. Oronan Researchers’ Mrs. Letecia S. Villanueva Research Coach Abstract The experience of human misery in the form of disease is perhaps as old as the inception
Premium Escherichia coli Bacteria Antibiotic resistance
Disposition/Food Safety: Overview of Food Microbiology July 8‚ 2011 Overview of Food Microbiology OBJECTIVES At the end of this module‚ you will be able to: 1. Explain the structural similarities and/or differences among Gram-positive and Gram-negative bacteria as well as their isolation and identification using serological‚ biochemical‚ and molecular techniques. 2. Identify the functions of the bacterial cell wall. 3. Identify the extrinsic and intrinsic parameters that affect bacterial growth
Premium Bacteria Microbiology Foodborne illness