Lab Report #1: Observing Bacteria Microbiology Abstract: This lab exercise familiarized the student with the use of a microscope by observing and identifying various different slides under the microscope. The student practiced observing the given slides under the 10x‚ 40x‚ and 100x (oil immersion) objective lenses‚ which allowed for the identification of the different organism’s shapes and sizes. Purpose: The aim of this exercise is to equip the student with the knowledge
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Purpose: Unknown mixtures will be separated by means of chromatography in which the mixture will be passed in a solution through a medium leaving behind components of the mixture at different rates‚ therefore‚ different spots on the absorbing substance. This will help determine the identity of unknown mixtures. The spot colors on the strip of filter paper and the Rf values of the unknown samples will be compared to those of known samples. To find the position of the spots on the strip of paper‚ we
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The first test conducted on unknown bacteria 32 was the Gram stain. From this stain‚ unknown 32 was found to be a Gram-positive cocci. This test eliminated all possible Gram-negative bacteria‚ Gram-positive rods and Gram-positive spirillium. Next‚ the endospore test determined whether or not the Gram-positive bacteria contained endospores. With the use of malachite green‚ steam‚ and safranin it was found that unknown bacteria 32 did not contain endospores. This eliminated Gram-positive cocci Sporosarcina
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1. Coagulase 2. this will differentiate Staphylococcus aureus from Staphylococcus epidermidis. 3. B. Staphylococcus aureus 4. the plasma clotted (gelled)‚ indicating a positive coagulase‚ which indicates S. aureus having followed the diagnostic scheme so far. the beta-hemolysis of this particular strain of S. aureus is not diagnostic of the species. Some strains produce the hemolysin‚ while others don’t. 5. B. Alpha hemolytic and small. (There is a greenish color of the red blood
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Abstract The primary focus of this lab was on microscopy and simple stains. Crystal violet and Carbol fuchsine‚ simple staining components‚ were used to stain the slide in order to see the different microbes in order to determine their cellular shape and identify unknown ones by comparing. Introduction Bacterial cells are usually colorless because cytoplasm‚ for the most part‚ is transparent. Since the bacteria are colorless‚ it is almost essential to add a stain to make the bacteria more visible
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performed this test‚ the initially slant for unknown microorganism #17 is important. For this lab‚ two identical slants are used for two reasons. Firstly‚ the slant can be used to make sure that there is no contamination from the Nutrient Agar plate. Secondly‚ the second slant will become a stock culture to prevent the shortage of slants during performing the series of tests. Kliger’s Iron Agar tests can be used to determine multiple characteristics of unknown microorganism #17. Kliger’s Iron Agar slants
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Shakira Jarvis Microbiology Lab Assignment Laboratory Assignment Outline 1. Check in & The Microscope a. Review of proper lab etiquette. i. Review laboratory syllabus and b. Review of the Parts of a Microscope ii. Review of lab exercises about different types of Microscopes 2. Survey of Microorganisms c. Viewing‚ drawing‚ and describing several types of fungi‚ algae‚ and Bacteria iii. Chlamydomonas iv. Spirogyra
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detect which organism we had in our unknown mixed culture tube by running a series of experiments to detect which specific Gram negative organism we had. To detect your gram positive from the mixed culture was given as extra credit points also. A Gram stain was performed and isolation streak plate in order to isolate and observe the unknown organism. Before the series of test‚ a dichotomous key had to be written up in order to know what steps and tests to run to identify the unknown Gram negative
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References: Koneman‚ E. W. (1997). Koneman ’s Color Atlas and Textbook of Diagnostic Microbiology. USA: Lippincott Williams & Wilkins. Madigan‚ et. al (2014). Brock Biology of Microorganisms (14th ed.‚ p. 78). Boston: Pearson. Mahon‚ C.‚ Lehman‚ D.‚ & Manuselis‚ G. (2011). Textbook of Diagnostic Microbiology. Missouri: Elsevier. Tille‚ P. M. (2014). Bailey & Scott ’s Diagnostic Microbiology. Missouri: Elsevier.
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Identifying an Unknown Aldehyde or Ketone Introduction The purpose of this lab is to identify an unknown aldehyde or ketone substance using chemical tests. The chemical tests used in this experiment are solubility‚ Schiff‚ Bisulfite‚ and Iodoform tests. Also‚ a 2‚4-dinitrophenylhydrazone derivative synthesis reaction will be completed from which a melting point will be obtained. The chemical test results and the melting point analysis will be compared to the table of compounds given to find the
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