Intro to Microbiology: Gram stain: Gram + Gram - Exotoxin Except Listeria has endotoxin Endotoxin Except Capsules Strep pneumo VERY Dangerous: (Some Strange Killers Have Pretty Nice Capsules) 1. Salmonella 2. Strep 3. Kleibsiella 4. Hemophilus B 5. Pseudomonas 6. Nisseria-(largest capsule- most likely cause of any toxicity) 7. Citroacter Gram (+) Gram (-) N-acetyl muramic acid (Nam) + N-acetyl glucosamine (Nag) = Peptidoglycan wall Contains
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Microbiology in the News: Fecal Transplants Zimmer’s article on fecal transplants (2016)‚ discusses how the effectiveness of transporting healthy stool to sick individuals has led to a series of unanswered questions and beliefs. For years scientists were unsuccessful in finding a use for this type of treatment. The breakthrough came about during a trial experiment using the procedure to treat a serious bacterial infection; Clostridium difficile. Clostridium difficile is a bacterium that causes
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cheese. Lactic acid bacteria(LAB)‚ a bacteria that can be found in the production of cheese‚ its stress gene was investigated in the experiment by using various biochemical and genetic techniques to identify and extract. The characterisation of the strain illustrates how identification of strains differ using different methods‚ such as gram stain and 16s rRNA screening. After the characterisation‚ the stress gene isolation assist the further understanding of the gene on LAB be giving different stress
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REVIEW SHEET‚ EXAM 3 Chapter 6‚ Nutrition and Microbial Growth Define the four major modes of nutrition in microorganisms and distinguish among them in terms of energy and carbon sources The four major modes of nutrition are: 1. Photoautotrophs: (ex. Plants‚ some protozoa‚ & alegae) -microorganism which use carbon dioxide as a carbon source and light energy from the environment to make their own food. 2. Chemoautotrophs: -microorganism which uses carbon dioxide as a carbon source and
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transfer of matter or energy across it 2. Undefined media - basal or complex media 3. Z-streak - is a technique used to isolate a pure strain from a single species of microorganism‚ often bacteria 4. Selective media – a growth medium or culture medium is a liquid or gel designed to support the growth of microorganisms or cells‚ or small plant 5. Differential media -are used to isolate or identify particular organisms. 6. Microaerophiles - A microaerophile is a microorganism that requires
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Master ’s-Prepared Nurse Interview Introduction I had the privilege to work for many years with couple of master’s prepared nurses and they always inspired me to join master’s level program. I interviewed one of my friends [S.V] who is a colleague at the hospital where I work as a requirement for the course and also to get a broader depth and understanding of the Masters in nursing program. That helped me to get more information about the program
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Getting Prepared to Write Research Paper Abstract- According to Jane Buxton‚ MD‚ of the British Columbia Center for Disease Control in Vancouver‚ and colleagues; Systematic reviews and meta-analysis of 124 published studies from 30 countries‚ found that people with tattoos were almost three times more likely to have hepatitis C as those without tattoos. Diseases such as hepatitis B‚ tuberculosis and HIV. Skin infections are also common from tattoos. Many of these causes are due to unhealthy
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lens while observing prepared bacterial slides. Additionally‚ it will be necessary to prepare slides so as to observe bacterial cultures from yogurt as well as to observe the composition of blood (i.e. red blood cells‚ white blood cells‚ and platelets). Procedure: Exercise 1: Viewing Prepared Slides To begin this lab experiment I first constructed my incubator using a small Styrofoam cooler and a standard 7 watt light bulb. Next‚ I read and reviewed the Science Lab Safety Reinforcement Agreement
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Introduction The purpose of this project was to detect which organism we had in our unknown mixed culture tube by running a series of experiments to detect which specific Gram negative organism we had. To detect your gram positive from the mixed culture was given as extra credit points also. A Gram stain was performed and isolation streak plate in order to isolate and observe the unknown organism. Before the series of test‚ a dichotomous key had to be written up in order to know what steps
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Mini-Lab Report Objectives: There were two major goals of the “Observing Bacteria and Blood” experiment. The first was to establish a familiarity with the proper techniques for using a microscope. The experiment focused on both the basic components of a microscope (i.e. how to adjust the knobs and levers for the desired result) as well as how do decide the proper objective to use for observing a specimen. The second goal major goal of this experiment was to practice observing live specimens
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