CHAPTER I INTRODUCTION 1.1 Background Food is one of human sources of calorie‚ protein‚ fats‚ and nutrition. Yet‚ because of the highly nutritious content‚ food is susceptible to growth of microorganisms. By the presence of microorganisms in food‚ the food is more likely to have shorter shelf life. Thus‚ mostly it is resolved by the addition of antimicrobial substances to food‚ such as condiments and preservatives. Condiments and preservatives could inhibit the growth of microorganisms
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Detection of plasmids in arsenic (As) resistant bacteria isolated from As-contaminated groundwater Mahima Rani and Pinaki Sar* Department of Biotechnology‚ Indian Institute of Technology‚ Kharagpur‚ West Bengal–721302‚ India mahima06bt11@gmail.com‚ Ph: +91 8348523016 Abstract Role of plasmids in conferring resistance to several heavy metals and antibiotics to naturally occurring bacteria is well known. In contaminated environments‚ presence of metal resistant genes on plasmids often provide
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and van Leeuwenhoek. 1-6 Compare spontaneous generation and biogenesis. 1-7 Identify the contributions to microbiology made by Needham‚ Spallanzani‚ Virchow‚ and Pasteur. 1-8 Explain how Pasteur’s work influenced Lister and Koch. 1-9 Identify the importance of Koch’s postulates. 1-10 Identify the importance of Jenner’s work. 1-11 Identify the contributions to microbiology made by Ehrlich and Fleming. 1-12 Define bacteriology‚ mycology‚ parasitology‚ immunology‚ and virology.
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Lab Practical 2 Morphological Staining Techniques Simple Stain- Uses 1 Stain 1. Acid Stains (- Charge)- Stains Background- Nigrosin‚ India Ink and Congo Red 2. Base Stains (+ Charge)- Stains Cell- Methylene Blue‚ Crystal Violet‚ Safranin Differential Staining Techniques- Any Staining Technique using 2 or more stains is differential. It allows us to differentiate between parts. 1. Gram Stain- Two Stains‚ PLUS Reagents- Distinguishes Chemical Composition of Cell Wall PG only (+ Purple)
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Labset Four Worksheet 1. Define the term “selectively toxic.” Why is it an important feature of antimicrobial agents? A substance or drug is selectively toxic if the agent used in it is able to kill the microorganism that it is meant to without harm to the host that has taken the drug. This is very important to microbial agents in that it enables these agents to inhibit a microorganism by interacting with microbial functions or structures different from those of the host; thereby showing little
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Micrbio Clicker questions: 1. Organisms that have a prokaryotic cell structure‚ no peptidoglycan in cell walls and unique cell membrane lipids would most likely be: A. Archaea 2. Who first published extensive observations of microorganisms? A. Anthony Von Leeuwenhoek 3. The primary use of Koch’s postulates is to? A. Demonstrate that a disease is caused by a specific microorganism 4. Microscope resolution ____________ as the wavelength of radiation used to illuminate the specimen
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found in human body‚ mainly microbes includes : bacteria‚archaea‚fungi and protists. U.S. Department of human and Health Services.2012: what are microbes.[online] ].[Accessed 9 April 2013] .Available from: world wide web:< http://www.niaid.nih.gov/topics/microbes/Pages/default.aspx>‚ majority of microbes are completely harmless but some of them do cause deadly diseases but it is a that humans cannot live without microbes but they can live even without us‚ these organisms play an important role in
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Introduction to Microbiology – Case Study 1 Kristen Alejos You are working as an emergency room nurse in Topeka when a mother brings in her 8 year old son because of severe‚ bloody diarrhea. When questioned‚ the boy said that it hurts when he goes to the bathroom and his mother said he had vomited in the previous two days. When asked about their previous movements‚ the mother and son both mentioned a backyard barbeque that had taken place 6 days earlier. The boy complained that his mother
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Glowing Transformations Abstract In this experiment‚ the idea is to become familiar with the transformation of cells. A well thought out procedure‚ involving a heat shock procedure‚ a good antibiotic‚ an inducer known as arabinose to show the newly expressed DNA by a visible fluorescent glow‚ and a stable control group is what contributes to this experiments thoroughness. It is predicted that the four agar plates will all yield different forms of growth‚ with different coloration and colony
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Introduction The science of microbiology is all about microorganisms‚ what they do and how they work. Microorganisms are ubiquitous‚ and are a very important part of life everywhere since they have been around for billions of years. The two main themes associated with microbiology are (1) having an understanding of the nature and function of the microbial world‚ and (2) being able to apply that understanding of the microbial world so that the benefits to the Earth and humans are known(6). This lab
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