References: • Bauman W‚ Robert. Microbiology with Diseases by Body System (3 ed).
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Microbiology Lab Report II Title: Determination of a Bacteriophage Titer Purpose: To determine the number of phage particles or plaque-forming units in a suspension of T4 bacteriophage. Materials: 18 24 hour broth culture of Escherichia coli B. 2 ml suspension of T4 bacteriophages with a titer of at least 10‚000 phages/ml 5 trypticase soy agar (TSA) plates. These should be warmed to 37c before use 5 tubes of soft agar (0.7% agar). Prior
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of Bacillus subtilis colonies on agar plates”. FEMS Microbiology Ecology 13: 159-167 4. Anna Csillag‚ 1972. “Appearance of acid-fast rods in cultures of mycococcus luteus”. Tubercle and Lung Disease 53: 221-225 5. James A. Bastock‚ Michelle Webb and Jane A. Grasby‚ 2007. “The pH dependence of the Escherichia coli RNase HII-catalysed Reaction Suggests that an Active Site Carboxylate Group Participates Directly in Catalysis”. Journal of microbiology 368: 421-433 6. Lloyd D. Witter‚ 1961. “Psychrophilic
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Investigating the Growth of P. fluorescens Biofilms at the Air-Liquid Interface and the Variations in Biofilm Integrity Abstract Pseudomonas fluorescens produces biofilms in a variety of environments‚ and in experimental situations has been shown to develop random mutations resulting in a phonotypical change in the resulting biofilm. One particular mutation resulted in the growth of a biofilm known as the Wrinkly Spreader‚ which produced a thicker‚ stronger biofilm that had a stronger attachment
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Bacterial Smears Are Fixed before Staining to? Answer It is important to heat fix the bacterial smear before staining so as to‚ kill the bacteria‚ firmly adhere the smear on to the microscopic slide to prevent washing off during staining‚ and to allow the sample to readily take up the stain. Reference: www2.hendrix.edu What is the purpose of heat- fixing the smear? It helps the cells adhere to the slide so that they can be stained. The purpose of heat fixing is to kill the organisms without
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understanding become apparent. In the study of living things‚ the Luria and Delbrück experiment stands out for its unique role in opening the door to genetics and microbiology. Though it initially made little impact on the natural sciences‚ it would come to be recognized as a cornerstone in the development of genetics and microbiology. Luria and Delbrücks fluctuation test showed that mutations arise absent from selection‚ consequently establishing that Darwinian natural selection acted on bacteria
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Cited: Centers for Disease Control and Prevention‚ Department of Health and Human Services Retrieved from:http://www.niaid.nih.gov/topics/anthrax/Pages/overview.aspx Cowan‚ M. K. (2012). Microbiology a Systems Approach . Mcgraw Hill. P306 BIOMED Central Retrieved From:http://www.biomedcentral.com/1471-2180/8/21
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References: Engelkirk‚ Paul G.‚ and Janet L. Duben-Engelkirk. "Medical Importance of the Family Enterobacteriaceae." Laboratory Diagnosis of Infectious Diseases: Essentials of Diagnostic Microbiology. Baltimore: Wolters Kluwer Health/Lippincott Williams & Wilkins‚ 2008. Microbiology: An Introduction. 10th Edition. Chapter 11 The Prokaryotes. Enterobacter. Pg 310. 2010. Library of Congress Cataloging-in-Publication Data. 0-321-74242-7.
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The Contributions of Louis Pasteur Sarah E. Gibson Haywood Community College BIO 175 IN1 Louis Pasteur is believed to be one of the most important contributors of microbiology. Pasteur was born in Dole‚ France on December 27‚ 1822 and died in Saint Cloud on September 28‚ 1895. Pasteur attended primary school in Arbois‚ France and he attended secondary school in Besancon. In 1840 Pasteur received his Bachelor of Arts and in 1842 he received his Bachelor of Science degree at the Royal College
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Weekly iLab iLab: Exploring the Microbiology Lab Section 1 Lab Safety 1. There are four safety equipment items that a lab should have. Identify two of these four items. (2 pts) Biological Safety Cabinet‚ Eyewash and shower 2. Identify one of the three ways to keep your work area safe. (1 pt) Keep your workspace free of all unnecessary materials 3. There are five recommendations for dressing properly in a lab environment. Name two of these recommendations. (2 pt) Avoid loose
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