cellulolytic fungi‚ several qualitative display of cellulolytic such congo red clearing zone assay‚ gel diffusion assay and dyed congo red filter paper clearing zone assay can be used. In this screening‚ congo red clearing zone assay is performed on 9 unknown fungi isolates on CMC media. Cellulose degradation and its subsequent utilizations are important for global carbon sources (Ponnambalam et al.‚ 2011). The hydrolysis of cellulose has become an interesting research and industrial research since it
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on the body of marine animals or internal organs of both aquatic and terrestrial animals. Amoeba reproduces asexually by binary and multiple fissions. They eat algae‚ bacteria‚ other protozoan‚ and tiny particles of dead plant or animal matter. Bacillus: They are rod shaped‚ gram positive bacteria found in soil and water. The largest are 2m across and 7m long. Most are aerobic but some under certain conditions can be facultative anaerobes. Each bacterium creates only one spore‚ which is resistant
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Title Antimicrobial properties in different type of plants. Introduction A substance that kills or prevents the growth of microorganisms for example bacteria‚ fungi or protozoans is called an antimicrobial. This substance has 2 major roles which are to either kill microbes (microbiocidal) or prevent the growth of microbes (microbiostatic). Disinfectants are antimicrobial substances used on non-living objects outside the body. This substance included antibiotics‚ antifungals‚ antiprotozoals and
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Effect of Osmotic Stress and Temperature on Microbial Growth BIO 3400-002L – Microbiology Lab 1 Effect of Osmotic Stress and Temperature on Microbial Growth Luiz Felipe Isidoro ABSTRACT Evolution allowed primitive forms of life to develop proteins and enzymes that made it possible for life to evolve under environments with hostile conditions‚ such as high salt and heat. More specifically‚ some bacteria selected genes that code for peptides with stronger intermolecular forces‚ coping
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papain and a microbial enzyme on meat proteins and beef Landmann‚ W.A. 1963. Enzymes and their influence on meat tenderness Balls‚ A.K.‚ and S.R. Hoover. 1937. The milk clotting action of papain McConn‚ J.D.‚ D. Tsuru‚ and K.T. Yasunobu. 1964. Bacillus subtilis neutral proteinase: I Cover‚ S.‚ R.L. Hostetler‚ and S.J. Ritchey. 1962. Tenderness of beef McKeith‚ F.K.‚ M.S. Brewer‚ and K.A. Bruggen. 1994. Effects on enzyme application on sensory‚ chemical‚ and processing Dapeau‚ G.R. 1976. Protease from
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Exercise 8-B Differential Staining Gram Staining and Acid Fast Staining Introduction: Differential Staining‚ one which facilitates differentiation of various elements in a specimen is a general term that can refer to a number of specific processes. Using multiple stains can better differentiate between different microorganisms or cellular components of a single organism. Gram’s Stain is a widely used method of staining bacteria as an aid to their identification. It was originally devised by Hans
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plate seemed to show that E. coli did not have lipases because it did not have clear zones. Staphylococcus aureus seemed to have these clear zones meaning it can secrete lipases. The bacteria Bacillus subtilis did not grow well to see if there was a clearing. After doing some research‚ it was shown that B. subtilis is lipase positive so there should have also been some clear sones like the Staphylococcus aureus (Watson). The DNAse agar could not have been analyzed because HCl was not available to precipitate
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Research in Biotechnology‚ 3(6): 51-58‚ 2012 ISSN: 2229-791X www.researchinbiotechnology.com Regular Article Isolation and identification of a new Bacillus strain for amylase production Yasser Bakri*1‚ Hassan Ammouneh1‚ Samir El-Khouri1‚ Muhanad Harba1 and Philippe Thonart2 1Department of Molecular Biology and Biotechnology‚ AECS‚ Damascus‚ Syria‚ P.O.Box 6091 Wallon de Biologie Industrielle‚ Unité de Bio-industrie‚ Faculté de AgroBiotechnologie de Gembloux‚ Université de Liège
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hence‚ this study. The disc diffusion method was used to determine the antibacterial activity against gram positive and gram negative bacteria (standard strains and clinical isolates). Antibacterial activity was tested on E. coli‚ S. aureus‚ B. subtilis and P. aeruginosa. Results show that there was no formation of any zone of inhibition as observed after 24 hrs. Thus‚ this justifies the traditional use of the plant as decoction treatment for various ailments. Various concentrations of crude
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Microbiology 197 Prepared Bacteria Gram Stains (F12) Materials required: * Microscope; clean and properly set up * Immersion oil * Lens paper * Lens cleaning fluid * Microscope drawing forms * Specimens: 1. Bacillus subtilis 2. Staphylococcus aureus. 3. Escherichia coli Procedure: 1. Observe each of slides listed in “Specimens” above. 2. Make your observations using oil immersion (1000X). 3. Using a drawing form draw the organisms
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