7351_2006_1_S03 Rajiv Gandhi University of Health Sciences‚ Karnataka PM.D. [MICROBIOLOGY] DEGREE EXAMINATION Time: 3 Hours [Max. Marks : 100] PAPER I – GENERAL MICROBIOLOGY AND IMMUNOLOGY QP Code: 7351 Your answers should be specific to the questions asked. Draw neat labeled diagrams wherever necessary. Answer all questions LONG ESSAYS 1. 2. 2 X 20 = 40 Marks Discuss the production‚ properties and uses of monoclonal antibodies Discuss various in vitro antibiotic sensitivity
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mobile phase. In TLC‚ the solvent went up at different rates. The retention factor (Rf) of a component can then be measured by dividing the distance that was traveled by the solvent front distance.By doing this experiment‚ the number of moles and grams of salicylic acid and acetic anhydride can be answered. Methods/ Material: Capillary tube TLC Plates Gloves 250 mL‚ 600 mL beakers Salicylic acid Acetic anhydride 85% phosphoric acid Buchner funnel Hot plate Filter paper 95 % of ethanol FeCl3 Glass
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Ahmad Shrab . Introduction This lab is purposed to familiarize basic equipment and techniques used in the study of microorganisms. In addition‚ learn some basic techniques used in identifying prokaryotes and make and view microscope slides of some common prokaryotes. In this lab ‚ I worked two experiments ‚ the first one is cultivation bacteria "colony "‚ and When microorganisms are cultivated
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these pathogens and their related epidemiology has become increasingly more important. The purpose of this study was to identify an unknown bacterium in a controlled laboratory environment over a 5 week period. Utilizing a variety of differential testing and staining methods learned in the microbiology course‚ students were to determine the identity of an assigned unknown organism. Observations were made and recorded each week to narrow down the scope of identification. Data has been presented in
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My partner and I were given unknown number 3 in the laboratory. After performing various tests over the course of a few weeks on our unknown‚ we came to the conclusion that our unknown organism is Klebsiella pneumoniae (K. pneumoniae). K. pneumoniae is a gram negative bacillus shaped microorganism. We observed that K. pneumoniae is a nonmotile organism. We performed multiple tests on our unknown culture‚ therefore we are very confident that it is correctly identified. We identified that K. pneumoniae
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Traditional Analysis of Unknown Colony Morphology Color- light yellow pigmentation Form- circular Elevation- convex Margin- entire [staphylococci] DNase Test This test is used to detect if the bacteria contains any deoxyribonuclease activity. Because no color change was observed from blue to clear my unknown bacteria displayed a negative result. Blood agar plate This test is used to detect the hemolytic activity in the bacteria. A darkish green color on the media around the
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Abstract: The Enzyme Lab results where when the liver was frozen‚ its reaction was fast‚ and when it was hot‚ it was slow‚ and the liver that was at room temperature reacted slowly to medium. Introduction: The Enzyme Lab is to conduct investigations to determine the most favorable conditions for the most efficient enzyme activity. Variables to be used testing include temperature‚ pH values and surface area. Enzymes are proteins that speed up the rate of chemical reactions‚ which would otherwise
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Purpose The purpose of the “Beanium” lab was to determine the average atomic mass of three different isotopes of beanium. During the lab beans were counted to determine mass of the sum of the beans. Each type of bean were weighed separately and then averaged together to determine the average atomic mass of beanium. Materials and Hazards Analytical balance‚ coffee filter‚ pencil‚ paper‚ cup‚ American beans‚ Nigerian beans‚ Mexican beans‚ calculator‚ and paper. Raw Data Bean Total Mass w/ Cup Number
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difference between a gram positive bacteria and gram negative bacteria is the differences in cell wall composition. Prokaryotes known as eubacteria have three basic forms: rods‚ cocci and spiral. The bacterial cell wall is the single most important contributor to cell shape. In addition to shape of cell wall‚ presence or absence of flagellum‚ and if present‚ positions of flagellum‚ the eubacteria can be classified according to Gram Stain. First and foremost‚ gram positive bacteria are
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Lab 4: Cell Structures and the Gram Stain Purpose: The purpose of this lab is to familiarize the student with the use of a compound microscope and the Gram Stain test. Students will review a brief video and slides to introduce the compound microscope. Students will also view a video about the Gram Stain procedure and then answer questions related to it. Hypothesis If I look at the slides I will see where the cell wall is and what lies inside it and in the Gram Stain if I watch the video I will
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